In Guadeloupe, the use between 1972 and 1993 of chlordecone, an organochlorine insecticide, has permanently contaminated the island’s soil, thus contaminating the food chain at its very beginning. There is today a strong societal requirement for an improved mapping of the contaminated zones. Given the extent of the areas to be covered, carrying out soil tests on each plot of the territory would be a long and expensive process. In this article, we explore a method of demarcating polluted areas. The approach adopted consists in carrying out, using surface water analyses, a hydrological delimitation that makes it possible to distinguish contaminated watersheds from uncontaminated ones. The selection of sampling points was based on the spatial analysis of the actual and potential contamination data existing at the beginning of the study. The approach was validated by soil analyses, after having compared the contamination data of the watersheds with the soil contamination data of the plots within them. The study thus made it possible to highlight new contaminated areas and also those at risk of contamination and to identify the plots to be targeted as a priority during future analysis campaigns by State services.

The detoxification process of trace metals in the estuarine burrowing crab Neohelice granulata, after previously being exposed to anthropogenic pressures in the field, is described for the first time. The objectives of this study were (a) to assess the metal content (Cd, Cu, Pb, Zn, Mn, Ni, Cr, Fe) in the sediments and the uptake of these elements in the hepatopancreas of N. granulata; (b) to quantify trace metal concentrations in the hepatopancreas before and after the detoxification experiment; and (c) to relate this information to metallothionein (MT) induction or reversibility. The detoxification assay was performed for 25 days with artificial seawater under controlled conditions in a culture chamber. The results showed higher uptake and bioaccumulation of Zn and Cu from the sediments, and the hepatopancreas exhibited increased levels of Zn and lower concentrations of the rest of the metals and MTs after the assay, mainly Fe and Mn that were significantly lower. We conclude that trace metals could be translocated to and accumulated in the hepatopancreas, the main metabolic organ, and then eliminated under controlled conditions with corresponding reversibility of MTs. Graphical abstract

Trimethylamine (TMA) is an odorous volatile organic compound emitted by industries. Algal-based biotechnologies have been proven as a feasible alternative for wastewater treatment, although their application to abate polluted air emissions is still scarce. This work comparatively assessed the removal of TMA in a conventional bacterial bubble column bioreactor (BC) and a novel algal-bacterial bubble column photobioreactor (PBC). The PBC exhibited a superior TMA abatement performance compared to the conventional BC. In this sense, the BC reached a removal efficiency (RE) and an elimination capacity (EC) of 78% and 12.1 g TMA m⁻³ h⁻¹, respectively, while the PBC achieved a RE of 97% and a EC of 16.0 g TMA m⁻³·h⁻¹ at an empty bed residence time (EBRT) of 2 min and a TMA concentration ~500 mg m⁻³. The outstanding performance of the PBC allowed to reduce the operating EBRT to 1.5 and 1 min while maintaining high REs of 98 and 94% and ECs of 21.2 and 28.1 g m⁻³·h⁻¹, respectively. Moreover, the PBC improved the quality of the gas and liquid effluents discharged, showing a net CO₂ consumption and decreasing by ~ 30% the total nitrogen concentration in the liquid effluent via biomass assimilation. A high specialization of the bacterial community was observed in the PBC, Mumia and Aquamicrobium sp. being the most abundant genus within the main phyla identified. GraphicalAbstract

Chemical contaminants are released from mining, domestic and industrial effluents into an aquatic environment. Sediments (n = 10) were collected with an Eckman grab at ten stations in the Densu Estuary for ecological risk assessment. The spatial distribution of organic characteristics and ecological risk of metals—zinc, lead, copper, mercury, iron, and manganese in sediment—were analyzed using standard methods. The organic parameters occurred in the ranges, as follows: % C, 0.76 to 2.05, % TN, 0.06 to 0.015; % TP, 0.44 to 1.38; and C/N, 12.31 to 34.81. The ranges of metal concentrations (mg/kg) were as follows: Fe, 201.10 to 720.90; Mn, 40.10 to 152.70; Zn, 7.3 to 158.3; Pb, 1.9 to 84.7; Cu 3.4 to 23.0; and Hg, 0.01 to 0.05. The mean concentration of metals in the sediment were Fe > Mn > Pb > Zn > Cu > Hg. The highest mean concentration of Fe suggested redox conditions in the Densu Estuary. There is a low contamination factor (CF) for five metals (Zn, Hg, Fe, Cu, and Mn) (CF < 1) to high contamination of Pb (3 < CF ≤ 6). The average Pb concentration was above local and geological backgrounds, suggesting an anthropogenic source of pollution from industrial and domestic effluents and agrochemicals. The sediment was extremely enriched by Pb (EF > 50) with a positive index of geoaccumulation (0 < Igeo ≤ 2) than other metals. There is considerable to a very high degree of contamination (DC) (3 ≤ DC ≥ 6) of metals in the sediment of Densu Estuary. The potential ecological risk index (≤ 40 PERI < 80) suggested a very low to moderate ecological risk of metal pollution. The study provides baseline knowledge on geochemical contamination in tropical estuarine systems for the development of effective chemical control strategies towards sustainable management of coastal waters.

Estuaries serve as nursery grounds for many marine fish species. However, increasing human activities within estuaries and surrounding areas lead to significant habitat quality degradation for the juveniles. In recent years, plastic pollution has become a global environmental issue as plastic debris are found in all aquatic environments with potential adverse impacts on marine biota. Given the important ecological role of estuaries and implications of microplastics (MP) in ecosystems, here we assess the occurrence, number, size, and polymer types of MP ingested by wild and caged juvenile European flounder (Platichthys flesus). We deployed caged fish for 1 month at five sites in three estuaries in the eastern English Channel. The Seine estuary, heavily impacted by manmade modifications and one of the most contaminated estuaries in Europe, was compared to two smaller estuaries (Canche and Liane) less impacted by industrial activities. We found that juvenile flounders (7–9 cm) were vulnerable to plastic ingestion. Seventy-five percent of caged fish and 58% of wild caught fish had the presence of MP items in their digestive tract. Fibers (69%) dominated in the fish’s digestive tract at all sites. An average of 2.04 ± 1.93 MP items were ingested by feral juvenile flounder and 1.67 ± 1.43 by caged juvenile flounder. For the caged fish, the three sites impacted by wastewater treatment plant (Liane, Le Havre harbor, and Rouen) were those with the highest percentage of individuals that has ingested MP items. Most of the isolated items were fibers and blue in color. Polymers identified by micro Raman spectroscopy were polycaprolactam, polyethylene terephthalate, and polyurethane. Although other environmental factors may have affected caged fish condition and mortality, we found no significant correlation with the number of ingested MP. However, the high occurrence of MP ingested by juvenile fish on nursery grounds raises concerns on their potential negative effects for fish recruitment success and population renewal. Finally, this study describes, for the first time, the feasibility of using caged juvenile fish as an assessing tool of MP contamination in estuarine nursery grounds.

Microplastics are one of the main environmental pollutants in marine ecosystems, and their presence in seawater is a consequence of the widespread use of plastic materials in modern commodities. This wide usage of plastics includes the employment of microspheres in common personal care products, which end up being ultimately released into the aquatic compartment. Known ecotoxicological effects of microplastics favoured the search for technologically viable and environmentally safer alternatives, such as paraffin wax microparticles, whose ecotoxicological risks have not been entirely characterized. To address this gap, the present study exposed mussels (Mytilus sp.) for 96 h to three densities (5 mg/L, 20 mg/L and 80 mg/L) of four size ranges (100–300 μm, 300–500 μm, 400–850 μm, and 800–1200 μm) of paraffin wax particles. Toxicological endpoints were the activities of four enzymes involved in key cellular processes, including antioxidant defence (catalase (CAT), glutathione reductase (GRed) and glutathione peroxidase (GPx)) and phase II metabolism (glutathione S-transferases (GSTs)), as well as lipid content and fatty acid profiles of the digestive gland. Significant interactions between the presence/absence of food and paraffin particle density were found, as food sometimes worked as a confounding factor in the analysed biomarkers. Despite this uncertainty, some overall patterns emerged. In general, smaller paraffin wax particles (100–300 μm) caused little effects on the activity of the four enzymes tested, whereas larger particles (800–1200 μm) caused significant effects on almost all biomarkers. CAT activity was enhanced in animals exposed to larger paraffin particles, whilst GPx activity was depressed; GRed activity was not affected by the exposure to paraffin particles. The activity of GSTs was enhanced, but only in one tested condition. No effects were observed in terms of the total lipid content and fatty acids of exposed animals. Overall, data obtained in this work suggest that, at densities of paraffin wax particles comparable to the levels found in the environment for microplastic beads, no toxicity is expected to occur in the tested mussel species, by measuring the here-assessed toxicological endpoints.

The endocrine system of crustaceans regulates the molt cycle with ecdysteroid hormones, mainly the 20-hydroxyecdysone (20-HE). Moreover, the molt process requires the action of chitinolytic enzymes (e.g., chitinase, chitobiase) to break down the old cuticle. However, endocrine disrupting compounds (EDC) are capable of altering their normal functioning. Glyphosate-based herbicides (GBH), such as Roundup®, the most widely used herbicides, are found in freshwater environments and have been considered EDC for many aquatic organisms. Therefore, this study examined the effects of environmentally relevant GBH concentrations (0.0065, 0.065, and 0.28 mg L⁻¹) on the 20-HE concentration and chitobiase activity in the decapod prawn Macrobrachium potiuna exposed for 14 days. Additionally, lipid peroxidation, a biomarker of membrane lipid degradation, was evaluated in hepatopancreas to assess cellular damage. Results showed that GBH decreased the 20-HE concentration in females at the two highest concentrations tested, while an increase was observed in males exposed to the highest GBH concentration. In addition, GBH also decreased chitobiase activity in males (all concentrations) and females (the two highest concentrations). Finally, GBH caused increased lipid peroxidation in males, indicating cellular damage in the hepatopancreas. In conclusion, this work suggests that GBH is an EDC for crustaceans by disrupting molting, which could lead to altered reproduction and thus population dynamics. Graphical abstract Decrease in the 20-HE concentration and chitobiase activity in muscle of males and females of the freshwater prawn Macrobrachium potiuna exposed to the herbicide Roundup® for 14 days

Filamentous fungi Trichoderma have been able to efficiently degrade fluoroquinolone antibiotics namely ciprofloxacin (CIP) and ofloxacin (OFL) as well as the fungicide climbazole (CLB) that are persistent in conventional activated sludge processes. All targeted compounds were biotransformed by whole cells of Trichoderma spp., exactly T. harzanium and T. asperellum, and biosorption played a limited role in their elimination. However, contrasting results were obtained with the two strains. T. asperellum was more efficient against CIP, with a 81% degradation rate in 13 days of incubation, while T. harzianum was more efficient against CLB, with a 91% degradation rate. While in the case of OFL, both strains showed same efficiency with degradation rate around 40%. Adding a cytochrome P450 enzyme inhibitor hardly resulted in the modification of degradation kinetics supporting the implication of extracellular enzymes in chemical biotransformation. Transformation products were identified by liquid chromatography–high resolution–mass spectrometry and transformation pathways were proposed. Biotransformation of selected compounds included hydroxylation, oxidation/reduction and N- and O-dealkylation reactions, similarly to those reported with white rot fungi. CIP underwent transformations at the piperazinyl ring through oxidation and conjugation reactions, while OFL mainly underwent hydroxylation processes and CLB carbonyl reduction into alcohol. Consequently, Trichoderma spp. likely possess a machinery of unspecific enzymes, which makes their application in removal of pharmaceutical and personal care products attractive.

The representativeness of laboratory studies of the fate of pesticides in soil in field conditions is questionable. This study aimed at comparing the dissipation and bioavailability to earthworms of two fungicides, dimoxystrobin (DMX) and epoxiconazole (EPX), over 12 months under laboratory and field conditions. In both approaches, the fungicides were applied to the same loamy soil as a formulated mixture at several concentrations. We determined total DMX and EPX concentrations in the soil using exhaustive extraction, their environmental availability using mild extraction and their bioavailability through internal concentrations in exposed earthworms. The initial fungicide application appeared as much better controlled in terms of dose and homogeneity in the laboratory than in the field. One year after application, a similar dissipation rate was observed between the laboratory and field experiments (ca 80% and 60% for DMX and EPX, respectively). Similarly, the ratio of available/total concentrations in soil displayed the same trend whatever the duration and the conditions (field or lab), EPX being more available than DMX. Finally, the environmental bioavailability of the two fungicides to earthworms was heterogeneous in the field, but, in the laboratory, the bioaccumulation was evidenced to be dose-dependent only for DMX. Our findings suggest that the actual fate of the two considered fungicides in the environment is consistent with the one determined in the laboratory, although the conditions differed (e.g., presence of vegetation, endogeic earthworm species). This study allowed better understanding of the fate of the two considered active substances in the soil and underlined the need for more research dedicated to the link between environmental and toxicological bioavailability.

The use antimicrobials for therapeutic and metaphylactic purpose in humans and agriculture exerts selective pressure on animal and environmental microbiota resulting in the survival and spread of antimicrobial resistance genes among bacteria and subsequent development of resistance in bacteria. Previous studies have shown that honey bees’ microbiota (Apis mellifera) can accumulate antimicrobial resistance genes in their microbiome and act as collectors and disseminators of resistance genes. The aim of this study was to investigate to what extent honey bees act as reservoir of select antimicrobial resistance genes. This study was conducted on 35 groups of bees. Bees were collected from 35 sites in Umbria, Italy. PCR was used to screen pooled ground bees’ specimens for genes that code for resistance against antimicrobials that are commonly used in humans and in veterinary medicine including aminoglycosides (aph), beta-lactams (blaZ), tetracycline (tetM) and sulphonamides (sul1 and sul2). Twenty-four samples out of 35 (68.57%) were positive for at least one antimicrobial resistance gene. Two samples were positive for the aph, 5.71%; eight for blaZ, 22.86%; three for tetM, 8.57%; ten for sul1, 28.57% and eighteen for sul2, 51.43%. Positivity to more than one antimicrobial resistance gene was observed in nine samples, 25.71%. The multivariate analysis identified “presence of farms nearby” as the factor most closely related to PCR positivity. Honey bees (Apis mellifera) from Umbria, Italy, carry antimicrobial resistance genes and can be used as indicators of the presence of resistance genes in the environment.