Slow nutrient turnover and destructed soil function were the main factors causing low efficiency in phytoremediation of heavy metal (HM)-contaminated soil. Soil ecoenzymatic stoichiometry can reflect the ability of soil microorganisms to acquire energy and nutrients, and drive nutrient cycling and carbon (C) decomposition in HM-contaminated soil. Therefore, for the first time, we used the enzymatic stoichiometry modeling to examine the microbial nutrient limitation in rhizospheric and bulk soil of different plants (Medicago sativa, Halogeton arachnoideus and Agropyron cristatum) near the Baiyin Copper Mine. Results showed that the main pollutants in this area were Cu, Zn, Cd, and Pb, while Cd and Zn have the greatest contribution according to the analysis of pollution load index (PLI). The activities of soil C-, nitrogen (N)-, and phosphorus (P)-acquiring enzymes in the rhizosphere of plants were significantly greater than that in bulk soil. Moreover, microbial C and P limitations were observed in all plant treatments, while the lower limitation was generally in the rhizosphere compared to bulk soil. The HM stress significantly increased microbial C limitation and decreased microbial P limitation, especially in the rhizospheric soil. The partial least squares path modeling (PLS-PM) further indicated that HM concentration has the greatest effects on microbial P limitation (−0.64). In addition, the highest enzyme activities and the lowest P limitation were observed in the rhizospheric and bulk soil of M. sativa, thereby implying that soil microbial communities under the remediation of M. sativa were steadier and more efficient in terms of their metabolism. These findings are important for the elucidation of the nutrient cycling and microbial metabolism of rhizosphere under phytoremediation, and provide guidance for the restoration of HM-contaminated soil.

Summertime ozone (O₃) pollution has frequently occurred in the Beijing–Tianjin–Hebei (BTH) region, China, since 2013, resulting in detrimental impacts on human health and ecosystems. The contribution of weather shifts to O₃ concentration variability owing to climate change remains elusive. By combining regional air chemistry model simulations with near-surface observations, we found that anthropogenic emission changes contributed to approximately 23% of the increase in maximum daily 8-h average O₃ concentrations in the BTH region in June–July–August (JJA) 2017 (compared with that in 2013). With respect to the weather shift influence, the frequencies, durations, and magnitudes of O₃ exceedance were consistent with those of the heat wave events in the BTH region during JJA in 2013–2017. Intensified heat waves are a significant driver for worsening O₃ pollution. In particular, the prolonged duration of heat waves creates consecutive adverse weather conditions that cause O₃ accumulation and severe O₃ pollution. Our results suggest that the variability in extreme summer heat is closely related to the occurrence of high O₃ concentrations, which is a significant driver of deteriorating O₃ pollution.

Accurate quantification of nanoplastics (NPs) in complex matrices remains a challenge, especially for biological samples containing high content of organic matters. Herein, a new method extracting and quantifying polystyrene (PS) NPs from biological samples was developed. The extraction included alkaline digestion, centrifugation, and cloud point extraction (CPE), and the quantification included gold nanoparticles formation and labeling on surfaces of the extracted NPs and thereafter measurement with single particle inductively coupled plasma mass spectrometry (SP-ICP-MS). Results show that 25% tetramethylammonium hydroxide solution was an effective alkaline digestion solution for biological matrices, and CPE after centrifugation (3000 rpm, 10 min) was applicable to purify and enrich PS NPs with different sizes (100 and 500 nm) and surface functionalities (-COOH and –NH₂ modifications) from the digestion solution. The efficiency of Au labeling on PS NPs surface was improved by about 70% in the presence of 100 μM cetyltrimethylammonium bromide. The performance of the quantification method was examined by extraction and measurement of PS NPs spiked in four representative organism samples including bacteria, algae, nematode, and earthworm, and was further validated by analyzing the accumulated PS NPs in exposed nematodes. Good recovery rates (65 ± 10%–122 ± 22%) were achieved for spiking levels of 5–50 μg g⁻¹; the limit of detection was 3.7 × 10⁷ particles g⁻¹, corresponding to the mass concentration of about 0.02 and 2.5 μg g⁻¹ for the 100 nm and 500 nm PS NPs, respectively. The established extraction and quantification methods are efficient and sensitive, providing a useful approach for further exploring the environmental behavior and toxicity of NPs.

Quinone is the important redox functional group for electron transfer capacity (ETC) of humic acid (HA). Lignin, as major component in corn straw, can be decomposed into phenol monomers, then oxidation into quinones for synthesis of HA during composting process. However, it is still unclear that the effects of type and variation characteristics of phenol monomers on redox characteristics of HA during straw composting process. In this study, p-hydroxybenzoic acid (P1), vanillic acid (P2), syringic acid (P3), p-hydroxy benzaldehyde (P4), 4-coumaric acid (P5), 4-hydroxyacetophenone (P6), ferulic acid (P7) and 4-hydroxy-3-methylacetophenone (P8) were recognized and clustered into three groups. The concentration of polyphenol presented a significant downward trend during the straw composting process. Based on the relationships among phenol monomers to ETC, electron donating capacity (EDC), electron accepting capacity (EAC) and quinone, we found that P1, P2, P3, P5 and P7 were significantly related to ETC, EDC and EAC of HA (P < 0.05). Furthermore, NH₄⁺-N and NO₃⁻-N were the main micro-environmental factors linking to ETC-related phenol monomers and redox characteristics of HA in straw composts (P < 0.05). Finally, two groups of core microflora that promoting the ETC-related phenol monomers and NH₄⁺-N, and ETC-related phenol monomers and NO₃⁻-N were identified by Mantel test, respectively. This study contributes a new insight for polyphenol way for redox capacity of HA in traditional composting and utilization of straw compost in contaminated environments.

Hemocytes are the main immune cells in bivalve mollusks and one of the sensitive targets for nanoparticle toxicity. Bivalve hemocytes consist of multiple functional heterogeneous cell types, but their different roles in immune system against foreign particles remain largely unknown. In order to clarify the different immune responses of hemocyte subpopulations to silver nanoparticles (AgNPs) and Ag ions, in this study, the Hong Kong oyster (Crassostrea hongkongensis) hemocytes were employed and separated into three subpopulations based on their cell size and granularity, including agranulocytes (R1), semigranulocytes (R2), and granulocytes (R3). We first demonstrated that AgNPs could rapidly enter into the oyster hemocytes within 3 h by phagocytosis process and resulted in different immune responses in hemocyte subpopulations. The most affected cell subtype by AgNPs was the granulocytes, followed by semigranulocytes, whereas agranulocytes were not affected following exposure to AgNPs. Interestingly, AgNPs induced the granule formation in semigranulocytes and further increased the proportion of granulocytes, whereas their ionic counterparts had no such effects on hemocyte composition, indicating the different detoxification mechanisms for nanoparticulate and ionic form. Following AgNP exposure, the dissolved Ag ions were accumulated in lysosomes and caused lysosomal dysfunction, indicating that lysosomes were the main targets for AgNP toxicity and the dissolved Ag ions were the main contributor of AgNP toxicity. Furthermore, AgNP exposure induced reactive oxygen production and impeded the lysosome function and phagocytosis in granulocytes, with impaired immunity system in oysters. Our study identified the different immune responses of oyster hemocyte subpopulations to AgNPs based on the in vitro short-term exposure assays, which may be applied to rapidly evaluate the ecotoxicological risks of different nanoparticles in aquatic systems.

Rapid urbanization and the aggregation of human activities in cities have resulted in large amounts of anthropogenic heat (AH) emission, which affects urban climate. Quantifying and assessing the AH emission values accurately and analyzing their spatial distribution characteristics is important to understand the energy exchange processes of urban areas. In this study, the high spatial resolution anthropogenic heat flux (AHF) quantification and spatial distribution analysis were conducted using multi-source data in the Beijing-Tianjin-Hebei region (BTH region) of China. First, the AH emission in district and city level were estimated using inventory method based on energy consumption and socio-economic statistical data; Then, AHF spatial quantification models were constructed based on high spatial resolution nighttime light (NTL) data and Point of interests (POI) data, and 130 m × 130 m gridded AHF quantification result in BTH region was realized; Finally, the potential numerical and spatial distribution patterns of AHF were analyzed using various indicators including contribution rate and aggregation index. The results show that: (1) The parameterized index constructed based on NTL and POI data shows a strong correlation with AHF, with R² ranging from 0.79 to 0.94 and a mean absolute error (MAE) value of 0.72 w·m⁻², which can be applied to the quantification of gridded AHF values with high resolution. The highest total AHF in the study area is 214 w·m⁻², and the average value is 2.24 w·m⁻². (2) Considering the sources of AHF, industrial emission sources in BTH region contribute the most to the total AHF, but commercial building emission sources in Beijing have a higher contribution, which can reach 33.8%. (3) Different types of AHF have different spatial aggregation levels. Commercial building emission and human metabolic emission have the highest aggregation level, and transportation emission has the lowest aggregation level.

Hot moments of nitrous oxide (N₂O) emissions induced by interactions between weather and management make a major contribution to annual N₂O budgets in agricultural soils. The causes of N₂O production during hot moments are not well understood under field conditions, but emerging evidence suggests that short-term fluctuations in soil oxygen (O₂) concentration can be critically important. We conducted high time-resolution field observations of O₂ and N₂O concentrations during hot moments in a dryland agricultural soil in Northern China. Three typical management and weather events, including irrigation (Irr.), fertilization coupled with irrigation (Fer.+Irr.) or with extreme precipitation (Fer.+Pre.), were observed. Soil O₂ and N₂O concentrations were measured hourly for 24 h immediately following events and measured daily for at least one week before and after the events. Soil moisture, temperature, and mineral N were simultaneously measured. Soil O₂ concentrations decreased rapidly within 4 h following irrigation in both the Irr. and Fer.+Irr. events. In the Fer.+Pre. event, soil O₂ depletion did not occur immediately following fertilization but began following subsequent continuous rainfall. The soil O₂ concentration dropped to as low as 0.2% (with the highest soil N₂O concentration of up to 180 ppmv) following the Fer.+Pre. event, but only fell to 11.7% and 13.6% after the Fer.+Irr. and Irr. events, which were associated with soil N₂O concentrations of 27 ppmv and 3 ppmv, respectively. During the hot moments of all three events, soil N₂O concentrations were negatively correlated with soil O₂ concentrations (r = −0.5, P < 0.01), showing a quadratic increase as soil O₂ concentrations declined. Our results provide new understanding of the rapid short response of N₂O production to O₂ dynamics driven by changes in soil environmental factors during hot moments. Such understanding helps improve soil management to avoid transitory O₂ depletion and reduce the risk of N₂O production.

Although cadmium (Cd) is a toxic heavy metal that reportedly causes liver injury, few studies have investigated biomarkers of Cd-induced liver injury. The purpose of this study is to investigate the role of bile acid (BA) in Cd-induced liver injury and determine reliable and sensitive biochemical parameters for the diagnosis of Cd-induced liver injury. In this study, 48 Sprague-Dawley rats were randomly divided into six groups and administered either normal saline or 2.5, 5, 10, 20, and 40 mg/kg/d cadmium chloride for 12 weeks. A total of 403 subjects living in either a control area (n = 135) or Cd polluted area (n = 268) of Dongdagou-Xinglong (DDGXL) cohort were included, a population with long-term low Cd exposure. The BA profiles in rats' liver, serum, caecal contents, faeces, and subjects' serum were detected using high-performance liquid chromatography-tandem mass spectrometry (HPLC–MS/MS). Changes in rats' and subjects' liver injury indices, rats' liver pathological degeneration, and rats' liver and subjects’ blood Cd levels were also measured. Cadmium exposure caused cholestasis and an increase in toxic BAs, leading to liver injury in rats. Among them, glycoursodeoxycholic acid (GUDCA), glycolithocholic acid (GLCA), taurolithocholic acid (TLCA), and taurodeoxycholate acid (TDCA) are expected to be potential biomarkers for the early detect of Cd-induced liver injury. Serum BAs can be used to assess Cd-induced liver injury as a simple, feasible, and suitable method in rats. Serum GUDCA, GLCA, TDCA, and TLCA were verified to be of value to evaluate Cd-induced liver injury and Cd exposure in humans. These findings provided evidence for screening and validation of additional biomarkers for Cd-induced liver injury based on targeted BA metabolomics.

Construction workers on highway rehabilitation projects can be exposed to a combination of traffic- and construction-related emissions. To assess the personal exposure a worker experiences, a portable battery-operated Air Quality Device (AQD) was utilised to measure emissions during normal construction operations of a major road rehabilitation project. Emissions measured were nitrogen dioxide (NO₂), Total Volatile Organic Compounds (TVOCs) and Particulate Matter (PM₁₀, PM₂.₅, and PM₁). The objective of the paper is to document the hazardous emissions that construction workers may be exposed to and allow for a basis of informed decision making to mitigate the risks of a road construction project. Most critically, this article is designed to raise awareness of the potential impact to a worker's wellbeing as well as highlight the need for further research. Through statistical analysis, asphalt paving was identified as the most hazardous activity in terms of exposure relative to other activities. This activity was further assessed using discrete-time Markov chain Monte Carlo simulations with results indicating a high probability that workers may be exposed to greater hazardous emission concentrations than measured. Limiting the distance to the source of emissions, large-scale use of warm-mix asphalt and reducing the idling times of construction vehicles were identified as practical mitigation measures to reduce exposure and aid in achieving zero-harm objectives. Finally, it is found that males are more susceptible to long-term implications of hazardous emission inhalation and should be more aware if the scenarios they might work in expose them to this.

Extensive algal bloom in the surface water is a pressing issue in Lake Dianchi that causes lake restoration to be difficult owing to complex and variable phosphorus (P) sources in the water column. P released from algae, suspended particles (SS), and sediment can provide sustainable P sources for algal blooms. However, little is known regarding the dynamic of P speciation in these substances from different sources. In this study, solution ³¹P nuclear magnetic resonance (³¹P NMR) and chemical sequential extraction were employed to identify P speciation in algae, SS, and sediment during different periods. Results showed that dissolved inorganic P (Pᵢ) directly accumulated in algae in the form of orthophosphate (ortho-P) and pyrophosphate (pyro-P). Algae preferentially utilized Pᵢ, followed by organic P (Pₒ) in the water column when the Pᵢ was insufficient during growth and reproduction. The ³¹P NMR spectra demonstrated that ortho-P, orthophosphate monoesters (mono-P), orthophosphate diesters (diester-P), and pyro-P dominated the P compounds across the samples tested. Increasing remineralization of SS mono-P driven by intense alkaline phosphatase activities was caused by increasing P needs of algae and pressure of P supply in the water column. The higher ratios of diester-P to mono-P in sediment (mean 0.55) than those in algae (mean 0.07) and SS (mean 0.11 in surface water, 0.14 in bottom water) suggested that the degradation and regeneration occurred within these P compounds during or after sedimentation. Pᵢ content in algae during growth and reproduction was controlled by its P absorption and utilization strategies. Results of this study provide insights into the dynamic cycling of P in algae, SS, and sediment, explaining the reason for algal blooms in the surface water with low concentrations of dissolved P.