Background, aim and scope In the region of the Apuseni Mountains, part of the Western Carpathians in Romania, metal mining activities have a long-standing tradition. These mining industries created a clearly beneficial economic development in the region. But their activities also caused impairments to the environment, such as acid mine drainage (AMD) resulting in long-lasting heavy metal pollution of waters and sediments. The study, established in the context of the ESTROM programme, investigated the impact of metal mining activities both from environmental and socioeconomic perspectives and tried to incorporate the results of the two approaches into an integrated proposition for mitigation of mining-related issues. Study site The small Certej catchment, situated in the Southern Apuseni Mountains, covers an area of 78 km². About 4,500 inhabitants are living in the basin, in which metal mining was the main economic sector. An open pit and several abandoned underground mines are producing heavy metal-loaded acidic water that is discharged untreated into the main river. The solid wastes of mineral processing plants were deposited in several dumps and tailings impoundment embodying the acidic water-producing mineral pyrite. Methods The natural science team collected samples from surface waters, drinking water from dug wells and from groundwater. Filtered and total heavy metals, both after enrichment, and major cations were analysed by inductively coupled plasma optical emission spectroscopy (ICP-OES). Major anions in waters, measured by ion chromatography, alkalinity and acidity were determined by titration. Solid samples were taken from river sediments and from the largest tailings dam. The latter were characterised by X-ray fluorescence and X-ray diffraction. Heavy metals in sediments were analysed after digestion. Simultaneously, the socioeconomic team performed a household survey to evaluate the perception of people related to the river and drinking water pollution by way of a logistic regression analysis. Results and discussion The inputs of acid mine waters drastically increased filtered heavy metal concentrations in the Certej River, e.g. Zn up to 130 mg L⁻¹, Fe 100 mg L⁻¹, Cu 2.9 mg L⁻¹, Cd 1.4 mgL⁻¹ as well as those of SO₄ up to 2.2 g L⁻¹. In addition, river water became acidic with pH values of pH 3. Concentrations of pollutant decreased slightly downstream due to dilution by waters from tributaries. Metal concentrations measured at headwater stations reflect background values. They fell in the range of the environmental quality standards proposed in the EU Water Framework Directive for dissolved heavy metals. The outflow of the large tailing impoundment and the groundwater downstream from two tailings dams exhibited the first sign of AMD, but they still had alkalinity. Most dug wells analysed delivered a drinking water that exhibited no sign of AMD pollution, although these wells were a distance of 7 to 25 m from the contaminated river. It seems that the Certej River does not infiltrate significantly into the groundwater. Pyrite was identified as the main sulphide mineral in the tailings dam that produces acidity and with calcite representing the AMD-neutralising mineral. The acid-base accounting proved that the potential acid-neutralising capacity in the solid phases would not be sufficient to prevent the production of acidic water in the future. Therefore, the open pits and mine waste deposits have to be seen as the sources for AMD at the present time, with a high long-term potential to produce even more AMD in the future. The socioeconomic study showed that mining provided the major source of income. Over 45% of the households were partly or completely reliant on financial compensations as a result of mine closure. Unemployment was considered by the majority of the interviewed persons as the main cause of social problems in the area. The estimation of the explanatory factors by the logistic regression analysis revealed that education, household income, pollution conditions during the last years and familiarity with environmental problems were the main predictors influencing peoples' opinion concerning whether the main river is strongly polluted. This model enabled one to predict correctly 77% of the observations reported. For the drinking water quality model, three predictors were relevant and they explained 66% of the observations. Conclusions Coupling the findings from the natural science and socioeconomic approaches, we may conclude that the impact of mining on the Certej River water is high, while drinking water in wells is not significantly affected. The perceptions of the respondents to pollution were to a large extent consistent with the measured results. Recommendations and perspectives The results of the study can be used by various stakeholders, mainly the mining company and local municipalities, in order to integrate them in their post-mining measures, thereby making them aware of the potential long-term impact of mining on the environment and on human health as well as on the local economy.

Introduction Several hot spots of severe freshwater pollution and sediment contamination (mostly heavy metals, polyaromatic hydrocarbons and polychlorinated biphenyls) have been identified in Serbia as the consequence of outdated environmental legislation, negligible amounts of properly treated waste waters and accidental spills. Discussion Since ecotoxicological methods have never been incorporated into risk assessment procedures, mandatory effluent discharge or ambient water monitoring programmes, ecotoxicological research, based on bioaccumulation studies, conventional ecotoxicological tests and, recently, biomarkers of exposure and effect have been restricted to independent small- to medium-scale studies, conducted, basically, to confirm, underline or oppose the results of chemical-based monitoring and to lament on inadequate environmental regulations/policy and management practice. Although hot and unresolved ecotoxicological problems still remain beyond the reach of ecotoxicological research currently conducted in Serbia, or are tackled only sporadically, it is to be expected that on-going research and institutional capacity building should, hopefully, increase the competence and competitiveness of scientific community and speed up the process of harmonisation of national environmental legislation and policy with European Union.

Background, aim and scope Sulphonated anthraquinones are precursors of many synthetic dyes and pigments, recalcitrant to biodegradation and thus not eliminated by classical wastewater treatments. In the development of a phytotreatment to remove sulphonated aromatic compounds from dye and textile industrial effluents, it has been shown that rhubarb (Rheum rabarbarum) and common sorrel (Rumex acetosa) are the most efficient plants. Both species, producing natural anthraquinones, not only accumulate, but also transform these xenobiotic chemicals. Even if the precise biochemical mechanisms involved in the detoxification of sulphonated anthraquinones are not yet understood, they probably have cross talks with secondary metabolism, redox processes and plant energy metabolism. The aim of the present study was to investigate the possible roles of cytochrome P450 monooxygenases and peroxidases in the detoxification of several sulphonated anthraquinones. Materials and methods Both plant species were cultivated in a greenhouse under hydroponic conditions, with or without sulphonated anthraquinones. Plants were harvested at different times and either microsomal or cytosolic fractions were prepared. The monooxygenase activity of cytochromes P450 toward several sulphonated anthraquinones was tested using a new method based on the fluorimetric detection of oxygen consumed during cytochromes P450-catalysed reactions. The activity of cytosolic peroxidases was measured by spectrophotometry, using guaiacol as a substrate. Results A significant activity of cytochromes P450 was detected in rhubarb leaves, while no (rhizome) or low (petioles and roots) activity was found in other parts of the plants. An induction of this enzyme was observed at the beginning of the exposition to sulphonated anthraquinones. The results also indicated that cytochromes P450 were able to accept as substrate the five sulphonated anthraquinones, with a higher activity toward AQ-2,6-SS (0.706 nkat/mg protein) and AQ-2-S (0.720 nkat/mg protein). An activity of the cytochromes P450 was also found in the leaves of common sorrel (1.212 nkat/mg protein (AQ-2,6-SS)), but no induction of the activity occurred after the exposition to the pollutant. The activity of peroxidases increased when rhubarb was cultivated in the presence of the five sulphonated anthraquinones (0.857 nkat/mg protein). Peroxidase activity was also detected in the leaves of the common sorrel (0.055 nkat/mg protein), but in this plant, no significant difference was found between plants cultivated with and without sulphonated anthraquinones. Discussion Results indicated that the activity of cytochromes P450 and peroxidases increased in rhubarb in the presence of sulphonated anthraquinones and were involved in their detoxification mechanisms. Conclusions These results suggest the existence in rhubarb and common sorrel of specific mechanisms involved in the metabolism of sulphonated anthraquinones. Further investigation should be performed to find the next steps of this detoxification pathway. Recommendations and perspectives Besides these promising results for the phytotreatment of sulphonated anthraquinones, it will be of high interest to develop and test, at small scale, an experimental wastewater treatment system to determine its efficiency. On the other hand, these results reinforce the idea that natural biodiversity should be better studied to use the most appropriate species for the phytotreatment of a specific pollutant.

Background, aim and scope Marine and coastal sediments can accumulate substantial concentrations of metals and hydrocarbons, yet the consequences of this contamination for exposed biota in situ can be difficult to establish. Here, we examine the hypothesis that exposure to contaminated sediments can lead to detrimental effects in sedimentdwelling species. The combination of chemical and biological assessment allows the identification of the impact of chemical contamination, and their use as assessment tools is becoming increasingly important. Materials and methods The study was applied to marine sediments from the Bay of Algeciras (S Spain) impacted by multiple, low-level contaminant inputs, and the Galician Coast (NW Spain), historically impacted by an oil spill (Prestige 2002), with two reference sites selected in UK and Spain. The common lugworm Arenicola marina was exposed in the laboratory for 14 days to the marine sediments, and a suite of biomarkers of sublethal toxicity was combined with analytical chemistry to test for relationships between sediment contamination and effect. Results Moderate to strong correlations between organics, metals, and biological responses were observed, with DNA damage as measured using the Comet assay forming the largest contribution toward the observed differences (p<0.05). The responses of worms from sites experiencing different contamination loads were clearly distinguishable. Discussion We show how a combination of multibiomarkers with analytical chemistry can be used to investigate the toxicity of marine sediments, enabling the differentiation of sites showing different types of contamination. There are clear relationships in sublethal assays that can be related to the putative mode of toxicity of the contaminants. Conclusions The use of A. marina in this way provides a sensitive, holistic approach to sediment toxicity assessment, enabling comparisons between oil-polluted sites to be quantified.

National audience | Given the good performance of geosynthetic material for drainage, it seems interesting to use these products to replace part or all of the drainage layer commonly used in landfill cells (silicate materials). The calculation showed that the replacement of a granular layer of 0.50 m by a drainage geosynthetic product is not always as easy as it seems: the maximum water head legally acceptable decreases with the thickness of the studied equivalent solution, which limits its ability to flow. ARCADIS and SITA DECTRA present here a case study on the Romagne-sous-Montfaucon landfill site for which a hybrid (solid granular + geosynthetic) was called. | Compte tenu des bonnes performances des géosynthétiques de drainage, il semble intéressant d'utiliser ces produits pour remplacer tout ou partie du massif drainant généralement utilisé en fond de casier d'installations de stockage de déchets. Le calcul montre que le remplacement d'une couche granulaire de 0,50 m par un géosynthétique de drainage n'est pas toujours aussi aisé qu'il y paraît : la charge maximale réglementairement acceptable diminue avec l'épaisseur du dispositif équivalent considéré, ce qui limite sa capacité de débit. Arcadis et Sita Dectra présentent ici une étude de cas sur le site de Romagne-sous-Montfaucon pour lequel un dispositif hybride (massif granulaire + géosynthétique) a été préconisé.

[Departement_IRSTEA]Eaux [TR1_IRSTEA]RIVAGE | National audience | The paper presents parts of the recent guide of recommendations "Mountain reservoirs". It makes a synthesis of a feedback on the behaviour and the pathology of geomembrane lining systems and gives the strong recommendations of the guide relative to these systems, in particular onto the structure support, the drainage inherent to the DEG and the covering structure. A general description of the guide ends the article. | L'article présente, à travers le récent guide de recommandations « Retenues d'altitude », la synthèse d'un retour d'expérience sur le comportement et la pathologie des dispositifs d'étanchéité par géomembrane (DEG) en altitude. Il donne les recommandations fortes du guide relatives à ces dispositifs, en particulier sur la structure support, le drainage inhérent au DEG et la structure de recouvrement. Un descriptif général du guide termine l'article.

[Departement_IRSTEA]Ecotechnologies [TR1_IRSTEA]TED [Axe_IRSTEA]TED-SOWASTE | National audience | Protecting geotextiles are used in the design of watertight bottom liners at landfill sites, above the géomembrane, to protect it against puncture during the installation of the drainage layer and, during the service life, to the weight of the waste to be put in place. To date, the results of the 2 standard tests (static and dynamic tests) given by the technical sheets for the protecting geotextiles are not sufficient to choose the good geotextile. The development of an experimental procedure to simulate in the lab the waste loading conditions is presented here; this procedure uses large size static damage tests and follows a previous study concerning the implementation dynamic conditions (Budka & al., 2006). | Les géotextiles sont utilisés dans la constitution des étanchéités des installations de stockage de déchets (ISD) pour protéger la géomembrane contre les effets du poinçonnement liés à la mise en ½uvre de la couche de drainage puis, en service, au poids des déchets sus jacents. À ce jour, les deux essais normalisés de poinçonnement, statique et dynamique, figurant sur les fiches techniques des géotextiles de protection ne permettent pas directement le choix du produit adapté. La mise au point d'une procédure d'étude des effets des sollicitations en service est présentée ici ; elle s'appuie sur des essais d'endommagement de grandes dimensions et fait suite à une étude déjà publiée sur l'effet des sollicitations de mise en ½uvre (Budka et al., 2006).

[Departement_IRSTEA]Ecotechnologies [TR1_IRSTEA]TED [Axe_IRSTEA]TED-SOWASTE | National audience | This papers aims at presenting the various lining and drainage structures including geosynthetics that can be encountered in mining applications like heap leach pads, tailing storage facilities and waste rock storage facilities. The question of design and durability of drainage and lining systems is then discussed as regards the impact of the large loads that can be encountered in mining applications. Then liquid and gas transfers are discussed together with the attenuation of metals in GCLs. | Cet article permet, après une présentation de différentes structures à base de géosynthétiques rencontrées dans les installations minières ,aires de lixiviation, zones de stockage de résidus de lixiviation,ainsi que stockages de stériles, de discuter des structures d'étanchéité et de drainage mises en place dans ces structures. On aborde ensuite la problématique du dimensionnement et de la durabilité des systèmes drainants et d'étanchéité sous les fortes contraintes mécaniques typiquement rencontrées dans les installations minières. Puis on discute les transferts liquides et gazeux ainsi que l'atténuation des métaux dans les géosynthétiques bentonitiques.