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Mitigative effects of natural and model dissolved organic matter with different functionalities on the toxicity of methylmercury in embryonic zebrafish
2019
Li, Dan | Xie, Lingtian | Carvan, Michael J. | Guo, Laodong
Dissolved organic matter (DOM) occurs ubiquitously in aquatic environments and plays an intrinsic role in altering the chemical speciation and toxicity of methylmercury (MeHg). However, interactions between MeHg and natural DOM remain poorly understood, especially at the functional group level. We report here the mitigative effects of three natural organic matter (NOM) and five model-DOM under different concentrations (0, 1, 3, 10, 30 and 100 mg-C/L) on the toxicity of MeHg in embryonic zebrafish (<4 h post-fertilization, hpf). NOM are those from the Mississippi River, Yukon River, and Suwannee River, while model-DOM include those containing thiosalicylic acid, L-glutathione, dextran, alginic acid, and humic acid. We selected a MeHg concentration (100 n-mol/L) that reduces the survival rate of embryos at 24 hpf by 18% and increases malformations at 72 and 96 hpf. In the presence of DOM, however, the malformation rates induced by MeHg can be mitigated to a different extent depending on DOM concentrations, specific functional groups, and/or specific components. Model DOM with aromatic thiols was the most effective at mitigating the effects of MeHg, followed by L-glutathione, carbohydrates, and humic acid. NOM also mitigated the toxicity of MeHg dependent on their composition and/or effective DOM components as characterized by fluorescence excitation-emission matrix techniques. Specifically, humic-like DOM components are more effective in reducing the MeHg toxicity in the embryonic zebrafish compared to protein-like components. Further studies are needed to elucidate the interactions between DOM and MeHg and the mitigative mechanisms at the molecular level.
Mostrar más [+] Menos [-]Changes in blood pressure associated with lead, manganese, and selenium in a Bangladeshi cohort
2019
Bulka, Catherine M. | Scannell Bryan, Molly | Persky, Victoria W. | Daviglus, Martha L. | Durazo-Arvizu, Ramon A. | Parvez, Faruque | Slavkovich, Vesna | Graziano, Joseph H. | Islam, Tariqul | Baron, John A. | Ahsan, Habibul | Argos, Maria
Heavy metal contamination is widespread in Bangladesh. Previous studies have observed lead increases blood pressure over time. However, the role of other metal contaminants and essential micronutrients, which could also adversely affect blood pressure or act as protective factors, is understudied.We therefore evaluated the associations of lead, manganese, and selenium with blood and pulse pressure trajectories.We prospectively followed placebo-assigned participants nested within a randomized trial for the prevention of arsenic-related skin cancer (n = 255). Blood lead, manganese, and selenium were measured at baseline; blood pressure was measured at baseline and at 3 biennial follow-up examinations. Mixed-effect linear regression models were used to estimate associations with average annual changes in systolic, diastolic, and pulse pressure.In models simultaneously adjusted for baseline blood lead, manganese, and selenium concentrations in addition to other potential confounders, lead was linearly associated with increases in systolic blood pressure, but not with diastolic blood pressure or pulse pressure. A non-linear association was observed for manganese, such that mid-range concentrations were associated with decreases in systolic, diastolic, and pulse pressure. Baseline selenium concentrations in the highest quartile were also associated with longitudinal decreases in both systolic and diastolic blood pressure, while null associations were observed with pulse pressure. In exploratory analyses, the combination of mid-range manganese and high selenium concentrations completely offset lead-associated increases in blood and pulse pressure.The results indicate a direct, linear association of lead exposure with systolic blood pressure, and manganese and selenium exposures within certain ranges may have a blood pressure-lowering effect in this population.
Mostrar más [+] Menos [-]Examining the responses of the zebrafish (Danio rerio) gastrointestinal system to the suspected obesogen diethylhexyl phthalate
2019
Buerger, Amanda N. | Schmidt, Jordan | Chase, Amanda | Paixao, Carla | Patel, Tejas N. | Brumback, Babette A. | Kane, Andrew S. | Martyniuk, Christopher J. | Bisesi, Joseph H.
Epidemiological evidence suggests that phthalate plasticizers may act as “obesogens”, which are chemicals that exacerbate obesity. The gastrointestinal (GI) system is the primary exposure route for phthalates, however, the relationship between phthalate-driven perturbations of GI system functions that can influence obesity has yet to be examined. To address this knowledge gap, we exposed Danio rerio (zebrafish) for 60 days to either (1) Control feeding (5 mg/fish/day), (2) Overfeeding (20 mg/fish/day) or (3) Overfeeding with diethyl-hexyl phthalate (DEHP) (20 mg/fish/day with 3 mg/kg DEHP). After 60 days, Overfed and Overfed + DEHP zebrafish had elevated body mass, and hepatosomatic and gonadosomatic indices. RNAseq analysis of the GI revealed enrichment of gene networks related to lipid metabolism in the Overfed + DEHP group. Many of the enriched networks were under transcriptional control of peroxisome proliferator activated receptor alpha (pparα), a known modulator of lipid metabolism, immune function, and GI function. Real-time PCR confirmed that pparα was overexpressed in the Overfed + DEHP zebrafish, further revealing a pathway by which DEHP may influence lipid metabolism via the GI. These data increase our understanding of phthalate-driven effects on GI function and lipid metabolism, identifying gut-specific gene networks that may drive phthalate-exacerbated obesity.
Mostrar más [+] Menos [-]Toxic responses of metabolites, organelles and gut microorganisms of Eisenia fetida in a soil with chromium contamination
2019
Tang, Ronggui | Li, Xiaogang | Mo, Yongliang | Ma, Yibing | Ding, Changfeng | Wang, Junsong | Zhang, Taolin | Wang, Xingxiang
The toxic sensitivity in different physiological levels of chromium (Cr) contaminated soils with environmentally equivalent concentrations (EEC) was fully unknown. The earthworm Eisenia fetida was exposed to a Cr-contaminated soil at the EEC level (referred to as Cr-CS) to characterize the induced toxicity at the whole body, organ, tissue, subcellular structure and metabolic levels. The results showed that the survival rate, weight and biodiversity of the gut microorganisms (organ) had no significant difference (p > 0.05) between control and Cr-CS groups. Qualitative histopathological and subcellular evaluations from morphology showed earthworms obvious injuries. The organelle injuries combined with the metabolic changes provided additional evidence that the Cr-CS damaged the nucleus and probably disturbed the nucleic acid metabolism of earthworms. 2-hexyl-5-ethyl-3-furansulfonate, dimethylglycine, betaine and scyllo-inositol were sensitive and relatively quantitative metabolites that were recommended as potential biomarkers for Cr-CS based on their significant weights in the multivariate analysis model. In addition, the relative abundance of Burkholderiaceae, Enterobacteriaceae and Microscillaceae of the earthworm guts in the Cr-CS group significantly increased, particularly for Burkholderiaceae (increased by 13.1%), while that of Aeromonadaceae significantly decreased by 5.6% in contrast with the control group. These results provided new insights into our understanding of the toxic effects of the EEC level of Cr contaminated soil from different physiological levels of earthworms and extend our knowledge on the composition and sensitivity of the earthworm gut microbiota in Cr contaminated soil ecosystems. Furthermore, these toxic responses from gut microorganisms to metabolites of earthworms provided important data to improve the adverse outcome pathway and toxic mechanism of the Cr-CS if the earthworm genomics and proteomics would be also gained in the future.
Mostrar más [+] Menos [-]Subcellular distribution of cadmium in a novel potential aquatic hyperaccumulator – Microsorum pteropus
2019
Lan, Xin-Yu | Yan, Yun-Yun | Yang, Bin | Li, Xin-Yuan | Xu, Fu-Liu
Microsorum pteropus is a novel potential Cd (cadmium) aquatic hyperaccumulator. In the present study, hydroponic experiments were conducted to assess the accumulation and subcellular distribution of Cd in the root, stem and leaf of M. pteropus. SEM (scanning electron microscopy) – EDX (energy dispersive X-ray fluorescence spectrometer) and TEM (transmission electron microscopy) were used to observe the ultrastructure of different tissues under 500 μM Cd exposure. After exposure to 500 μM Cd for 7 days, the root, stem and leaf of M. pteropus can accumulate to be > 400 mg/kg Cd in dry mass with no significant influence on the growth. In the root and leaf of M. pteropus, the Cd was more likely to store in the cell wall fraction. However, Cd in the stem was mainly stored in both the cell wall fraction and the cytoplasm fraction. Under SEM observation and EDX detection, 1) Cd was found to be sequestrated in the epidermis or chelated in the root cells, 2) no significant deposit spots were observed in the stem, 3) Cd was found in the trichome of the leaf, and the sporangium was not damaged. TEM observations revealed 1) possible Cd precipitations in the root cell and 2) no significant ultrastructure variation in the stem, and 3) the chloroplast retained its structure and was not affected by the Cd. M. pteropus showed great capacity for Cd accumulation without influencing growth. In addition, the ultrastructure of all the tissues was not damaged by the Cd. M. pteropus showed a great potential in phytoremediation in heavy metal polluted water solutions, and may provide new directions for the study of resistance mechanisms of aquatic hyperaccumulators.
Mostrar más [+] Menos [-]The climatology of aerosol optical thickness and radiative effects in Southeast Asia from 18-years of ground-based observations
2019
Khan, Rehana | Kumar, Kanike Raghavendra | Zhao, Tianliang
The present study utilizes 18 years of long-term (2001–2018) data collected from six active AERONET sites over the Indo-Gangetic Plain (IGP) and the North China Plain (NCP) areas in Southeast Asia. The annual mean (±SD) aerosol optical thickness at 440 nm (AOT₄₄₀) was found high at XiangHe (0.92 ± 0.69) and Taihu (0.90 ± 0.51) followed by Beijing (0.81 ± 0.69), Lahore (0.81 ± 0.43), and Kanpur (0.73 ± 0.35) and low at Karachi (0.52 ± 0.23). Seasonally, high AOT₄₄₀ with corresponding high Ångström exponent (ANG₄₄₀₋₈₇₀) noticed during JJA for all sites, except Kanpur, suggesting the dominance of fine-mode particles, generally associated with large anthropogenic emissions. Climatologically, an increasing (decreasing) trend was observed over IGP (NCP) sites, with the highest (lowest) percentage of departures in AOT₄₄₀ found over Beijing (Karachi). We further identified major aerosol types which showed the dominance of biomass burning, urban-industrial followed by the mixed type of aerosols. In addition, single scattering albedo (SSA), asymmetry parameter (ASP), volume size distribution (VSD), and complex aerosol refractive index (RI) showed significant temporal and spectral changes, illustrating the complexity of aerosol types. At last, the annual mean direct aerosol radiative forcing at the top, bottom, and within the atmosphere for all sites were found in the range from −17.36 ± 3.75 to −45.17 ± 4.87 W m⁻², -64.6 ± 4.86 to −93.7 ± 10.27 W m⁻², and 40.5 ± 6.43 to 68.25 ± 7.26 W m⁻², respectively, with an averaged atmospheric heating rate of 0.9–2.3 K day⁻¹. A large amount of anthropogenic aerosols showed a significant effect of heating (cooling) on the atmosphere (surface) results obviously, due to an increased rate of atmospheric heating. Therefore, the thermodynamic effects of anthropogenic aerosols on the atmospheric circulation and its structure should be taken into consideration for future study over the experimental sites.
Mostrar más [+] Menos [-]Microplastic contamination and pollutant levels in mussels and cockles collected along the channel coasts
2019
Hermabessiere, Ludovic | Paul Pont, Ika | Cassone, Anne-Laure | Himber, Charlotte | Receveur, Justine | Jezequel, Ronan | El Rakwe, Maria | Rinnert, Emmanuel | Rivière, Gilles | Lambert, Christophe | Huvet, Arnaud | Dehaut, Alexandre | Duflos, Guillaume | Soudant, Philippe
Nowadays, environmental pollution by microplastics (<5 mm; MP) is a major issue. MP are contaminating marine organisms consumed by humans. This work studied MP contamination in two bivalve species of commercial interest: blue mussel (Mytilus edulis) and common cockle (Cerastoderma edule) sampled on the Channel coastlines (France). In parallel, 13 plastic additives and 27 hydrophobic organic compounds (HOC) were quantified in bivalves flesh using SBSE-TD-GS-MS/MS to explore a possible relationship between their concentrations and MP contamination levels. MP were extracted using a 10% potassium hydroxide digestion method then identified by μ-Raman spectroscopy. The proportion of contaminated bivalves by MP ranged from 34 to 58%. Blue mussels and common cockles exhibited 0.76 ± 0.40 and 2.46 ± 1.16 MP/individual and between 0.15 ± 0.06 and 0.74 ± 0.35 MP/g of tissue wet weight. Some HOC and plastic additives were detected in bivalves. However, no significant Pearson or Spearman correlation was found between MP loads and plastic additives or HOC concentrations in bivalve tissues for the two species.
Mostrar más [+] Menos [-]Monitoring of explosive residues in lake-bottom water using Polar Organic Chemical Integrative Sampler (POCIS) and chemcatcher: determination of transfer kinetics through Polyethersulfone (PES) membrane is crucial
2019
Estoppey, Nicolas | Mathieu, Jörg | Gascon Diez, Elena | Sapin, Eric | Delémont, Olivier | Esseiva, Pierre | de Alencastro, Luiz Felippe | Coudret, Sylvain | Folly, Patrick
Between 1920 and 1967, approximatively 8200 tons of ammunition waste were dumped into some Swiss lakes. This study is part of the extensive historical and technical investigations performed since 1995 by Swiss authorities to provide a risk assessment. It aims to assess whether explosive monitoring by passive sampling is feasible in lake-bottom waters. Polar organic chemical integrative sampler (POCIS) and Chemcatcher were first calibrated in a channel system supplied with continuously refreshed lake water spiked with two nitroamines (HMX and RDX), one nitrate ester (PETN), and six nitroaromatics (including TNT). Exposure parameters were kept as close as possible to the ones expected at the bottom of two affected lakes. Sixteen POCIS and Chemcatcher were simultaneously deployed in the channel system and removed in duplicates at 8 different intervals over 21 days. Sorbents and polyethersulfone (PES) membranes were separately extracted and analyzed by UPLC-MS/MS. When possible, a three-compartment model was used to describe the uptake of compounds from water, over the PES membrane into the sorbent. Uptake of target compounds by sorbents was shown not to approach equilibrium during 21 days. However, nitroaromatics strongly accumulated in PES, thus delaying the transfer of these compounds to sorbents (lag-phase up to 9 days). Whereas sampling rate (RS) of nitroamines were in the range of 0.06–0.14 L day⁻¹, RS of nitroaromatics were up to 10 times lower. As nitroaromatic accumulation in PES was integrative over 21 days, PES was used as receiving phase for these compounds. The samplers were then deployed at lake bottoms. To ensure that exposure conditions were similar between calibration and field experiments, low-density polyethylene strips spiked with performance reference compounds were co-deployed in both experiments and dissipation data were compared. Integrative concentrations of explosives measured in the lakes confirmed results obtained by previous studies based on grab sampling.
Mostrar más [+] Menos [-]In vivo solid-phase microextraction sampling combined with metabolomics and toxicological studies for the non-lethal monitoring of the exposome in fish tissue
2019
Roszkowska, Anna | Yu, Miao | Bessonneau, Vincent | Ings, Jennifer | McMaster, Mark | Smith, Richard | Bragg, Leslie | Servos, Mark | Pawliszyn, Janusz
Various environmental studies have employed the biomonitoring of fish in their aquatic ecosystems in order to identify potential metabolic responses to the exposome. In this study, we applied in vivo solid-phase microextraction (SPME) to perform non-lethal sampling on the muscle tissue of living fish to extract toxicants and various endogenous metabolites. Sixty white suckers (Catastomus commersonii) were sampled from sites upstream, adjacent, and downstream from the oil sands development region of the Athabasca River (Alberta, Canada) in order to track their biochemical responses to potential contaminants. In vivo SPME sampling facilitated the extraction of a wide range of endogenous metabolites, mainly related to lipid metabolism. The obtained results revealed significant changes in the levels of numerous metabolites, including eicosanoids, linoleic acids, and fat-soluble vitamins, in fish sampled in different areas of the river, thus demonstrating SPME's applicability for the direct monitoring of exposure to different environmental toxicants. In addition, several classes of toxins, including petroleum-related compounds, that can cause serious physiological impairment were tentatively identified in the extracts. In vivo SPME, combined with the analysis of contaminants and endogenous metabolites, provided important information about the exposome; as such, this approach represents a potentially powerful and non-lethal tool for identifying the mechanisms that produce altered metabolic pathways in response to the mixtures of different environmental pollutants.
Mostrar más [+] Menos [-]Life in a polluted world: A global review of anthropogenic materials in bird nests
2019
Jagiello, Zuzanna | Dylewski, Łukasz | Tobolka, Marcin | Aguirre, José I.
Human pressure exerts a significant influence on animals and the environment. One of its consequences, plastic pollution is considered one of the major threats to fauna as well as a significant conservation issue. In this research, we examined the global pattern of one example of avian behavior in response to pollution—namely, the incorporation of anthropogenic materials into nests—as well as the existing knowledge on this subject. Based on 25 articles, we studied 51 populations, involving 24 bird species, and checked 10,790 nests; as a result, we found that incorporation of debris is correlated with increasing human influence on the environment, measured as the Human Footprint Index. Moreover, the probability of debris incorporation is higher in terrestrial than in marine species. We also identified knowledge bias in favor of marine as opposed to terrestrial species: namely, marine species attract more scientific attention than terrestrial. Furthermore, research approaches to these two ecosystems differ. Undeniably, the factors which influence debris incorporation by birds, the scale of this behavior, and particular forms of use of debris in bird nests are aspects which require long-term standardized research. This is the first global review paper on debris incorporation by birds to demonstrate a close link to human pressure as a driver.
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