Although widespread antibiotic resistance has been mostly attributed to the selective pressure generated by overuse and misuse of antibiotics, recent growing evidence suggests that chemicals other than antibiotics, such as certain metals, can also select and stimulate antibiotic resistance via both co-resistance and cross-resistance mechanisms. For instance, tetL, merE, and oprD genes are resistant to both antibiotics and metals. However, the potential de novo resistance induced by heavy metals at environmentally-relevant low concentrations (much below theminimum inhibitory concentrations [MICs], also referred as sub-inhibitory) has hardly been explored. This study investigated and revealed that heavy metals, namely Cu(II), Ag(I), Cr(VI), and Zn(II), at environmentally-relevant and sub-inhibitory concentrations, promoted conjugative transfer of antibiotic resistance genes (ARGs) between E. coli strains. The mechanisms of this phenomenon were further explored, which involved intracellular reactive oxygen species (ROS) formation, SOS response, increased cell membrane permeability, and altered expression of conjugation-relevant genes. These findings suggest that sub-inhibitory levels of heavy metals that widely present in various environments contribute to the resistance phenomena via facilitating horizontal transfer of ARGs. This study provides evidence from multiple aspects implicating the ecological effect of low levels of heavy metals on antibiotic resistance dissemination and highlights the urgency of strengthening efficacious policy and technology to control metal pollutants in the environments.

Most of the previous studies of microplastic pollution on coastal habitats focused on high energy beaches although low energy areas such as mudflats are supposed to retain more microplastics, not to mention that mudflats are biologically more diverse. We quantified and characterized microplastics from 10 mudflats and 10 sandy beaches in Hong Kong spanning from the eastern to western waters. Sediment samples were collected at 1.0 m and 1.5 m above chart datum (CD) and at the strandline. Abundance of microplastics ranged between 0.58 and 2116 items kg−1 sediment with that on mudflats being ten times more than on beaches. Polyethylene (46.9%) was the most abundant and followed by polypropylene (13.8%) and polyethylene terephthalate (13.5%). Expanded polystyrene was the most abundant in the strandline samples but not at 1.0 m and 1.5 m above CD. Although previous studies have concluded that the input from Pearl River is a major source of microplastics on Hong Kong shores, this study has demonstrated that the contribution of local pollution sources such as discharge from sewage treatment plants to microplastic pollution should not be neglected.

Tetracyclines are a class of antimicrobials frequently found in the environment, and have promoted the proliferation of antibiotic resistance. An unanswered research question is whether tetracycline sorbed to soils is still bioavailable to bacteria and exerts selective pressure on the bacterial community for the development of antibiotic resistance. In this study, bioreporter E. coli MC4100/pTGM strain was used to probe the bioavailability of tetracycline sorbed by smectite clay, a class of common soil minerals. Batch sorption experiments were conducted to prepare clay samples with a wide range of sorbed tetracycline concentration. The bioreporter was incubated with tetracycline-sorbed clay at different clay/solution ratios and water contents, as well as using dialysis tubings to prevent the direct contact between bacterial cells and clay particles. The expression of antibiotic resistance genes from the bioreporter was measured using a flow cytometer as a measurement of bioavailability/selective pressure. The direct contact of bioreporter cells to clay surfaces represented an important pathway facilitating bacterial access to clay-sorbed tetracycline. In clay-water suspensions, reducing solution volume rendered more bacteria to attach to clay surfaces enhancing the bioavailability of clay-sorbed tetracycline. The strong fluorescence emission from bioreporter cells on clay surfaces indicated that clay-sorbed tetracycline was still bioavailable to bacteria. The formation of biofilms on clay surfaces could increase bacterial access to clay-sorbed tetracycline. In addition, desorption of loosely sorbed tetracycline into bulk solution contributed to bacterial exposure and activation of the antibiotic resistance genes. Tetracycline sorbed by soil geosorbents could exert selective pressure on the surrounding microbial communities via bacterial exposure to tetracycline in solution from desorption and to the geosorbent-sorbed tetracycline as well.

This study developed a lightweight air composition measuring equipment (ACME) mounted in unmanned aerial vehicles (UAVs) to measure the vertical distribution characteristics of PM₂.₅ chemical species in the micro-scale urban environment for the first time. 212 samples collected from 0 to 350 m above ground level were analyzed for water-soluble ions. The concentrations of most ions on the above ground level were higher than that on the ground surface during the sampling period. The measurements of the total ion concentrations were approximately 54 to 26% of the PM₂.₅ mass concentrations on the ground surface. The concentrations of NH₄⁺ and NO₃⁻ decreased with increases in the height from the ground, which may be related to the influence of the vehicle emissions and human activities. NO₂⁻ and SO₄²⁻ both had a peak concentration on the higher vertical altitude at night in the sea-land wind system. In the southern wind system, the emissions of sea salts, dust, and stationary pollution, might be transported by the regional prevailing airflow from the southern coastal area, were the major pollutant sources above the boundary layer. The vertical distribution of ionic concentrations and wind field provided information concerning changes in pollutant transport and source regions that affect the local air quality. The ACME mounted in UAVs is the feasible and convenient method to fast understand the vertical distributions of aerosol chemical species. It provides important information about the accumulation and diffusion effects by the boundary layer variation to aerosol characteristics, which is difficulty observed from the conventional ground-based measurements. In future, this technology is the useful application for investigating the pollutant species emitted from the smokestack and the sudden pollution accident.

Long-term genotoxic effects of two auxinic herbicide formulations, namely, the 58.4% 2,4-dichlorophenoxyacetic acid (2,4-D)-based DMA® and the 57.7% dicamba (DIC)-based Banvel® were evaluated on Cnesterodon decemmaculatus. Primary DNA lesions were analyzed by the single-cell gel electrophoresis methodology. Two sublethal concentrations were tested for each herbicide corresponding to 2.5% and 5% of the LC50₉₆ₕ values. Accordingly, fish were exposed to 25.2 and 50.4 mg/L or 41 and 82 mg/L for 2,4-D and DIC, respectively. Fish were continuously exposed for 28 days with replacement of test solutions every 3 days. Genotoxicity was evaluated in ten individuals from each experimental point at the beginning of the exposure period (0 day) and at 7, 14, 21 and 28 days thereafter. Results demonstrated for first time that 2,4-D-based formulation DMA® induced primary DNA strand breaks after 7–28 days exposure on C. decemmaculatus regardless its concentration. On the other hand, DIC-based formulation Banvel® exerted its genotoxic effect after exposure during 7–14 days and 7 days of 2.5 and 5% LC50₉₆ₕ, respectively. The present study represents the first evidence of primary DNA lesions induced by two widely employed auxinic herbicides on C. decemmaculatus, namely 2,4-D and DIC, following long-term exposure.

Although the abiotic and biotic transformation/degradation (T/D) processes of tetrabromobisphenol A (TBBPA) have been widely investigated in model experiments, few reviews have focused on these processes along with their metabolites or degradation products. In this paper, we summarize the current knowledge on the T/D of TBBPA and its derivatives, including abiotic and biotic T/D strategies/conditions, mechanisms, metabolites and environmental occurrences. Various treatments, such as pyrolysis, photolysis, chemical reactions and biotransformation, have been employed to study the metabolic mechanism of TBBPA and its derivatives and to remediate associated contaminated environments. To date, more than 100 degradation products and metabolites have been identified, dominated by less brominated compounds such as bisphenol A, 2,6-dibromo-4-isopropylphenol, 2,6-dibromo-4-hydroxyl-phenol, 2,6-dibromophenol, isopropylene-2,6-dibromophenol, 4-(2-hydroxyisopropyl)-2,6-dibromophenol, etc. It can be concluded that the T/D of TBBPA mainly takes place through debromination and β-scission. In some environmental media and human and animal tissues, brominated metabolites, glucoside and sulfate derivatives are also important T/D products. Here, the T/D products of TBBPA and its derivatives have been most comprehensively presented from the literature in recent 20 years. This review will enhance the understanding of the environmental behaviors of TBBPA-associated brominated flame retardants along with their ecological and health risks.

The eutrophication of estuaries results from increasing anthropogenic nutrient inputs to coastal waters. Ecosystem recovery from eutrophication is partly dependent on the ability of a system to assimilate or remove nutrients, and denitrification and dissimilatory nitrate reduction to ammonium (DNRA) are important pathways for nitrogen (N) removal or retention. We measured rates of denitrification and DNRA over an annual cycle at two stations in Weeks Bay, AL, a shallow microtidal estuary receiving freshwater from two rivers with agricultural watersheds and high N inputs. We hypothesized that rates of DNRA would exceed denitrification in the sulfidogenic sediments in this estuary. Consistent with our hypothesis, we found that DNRA (44.4 ± 5.5 μmol N m−2 hr−1) exceeded in situ denitrification (0.9 ± 2.3 μmol N m−2 hr−1) and that even in the presence of abundant water column nitrate DNRA was favored over denitrification by a factor of two. DNRA is estimated to provide N to the water column at a rate equivalent to 15% of the N input that is retained within the estuary and is a significant component of the N budget in this highly impacted estuary. DNRA by retaining N in the system contributes to the N demand by primary producers and can impact this estuary through enhanced rates of primary production. Weeks Bay, like many coastal estuaries, experiences periods of hypoxia, blooms of harmful algae and fish kills. Future management efforts should focus on reducing nutrient input to this estuary without which the significant retention of N in this system through DRNA will contribute to the undesirable ecosystem attributes associated with eutrophication.

The widespread application of organophosphorous flame retardants (OPFRs) has led to considerable human exposure, with major concerns regarding their health risks. Herein, we investigate the effects of triphenyl phosphate (TPP), one of the most widely used OPFRs, and one of its main metabolite diphenyl phosphate (DPP) on the endocrine systems and metabolic profiles after neonatal exposure from postnatal days 1–10 at two dosages (2 and 200 μg per day). Both TPP and DPP had no negative effect on uterine weight, glucose tolerance, and estradiol. 1H-NMR-based metabolomics revealed a sex-specific metabolic disturbance of TPP. Specifically, low dose of TPP altered the metabolic profiles of male mice while exerting no significant effects on female ones. Furthermore, a dose-dependent effect of TPP in male mice was observed, where a low toxicity dose up-regulated lipid-related metabolites, while a high toxicity dose down-regulated the pyruvate metabolism and TCA cycles. These results highlight the importance of carefully assessing the health impact of TPP on infants.

This study investigated the treatment performance and nitrogen removal mechanism of highly alkaline ammonia-stripped digestate effluent in horizontal subsurface flow constructed wetlands (CWs). A promising nitrogen removal performance (up to 91%) was observed in CWs coupled with intensified configurations, i.e., aeration and effluent recirculation. The results clearly supported that the higher aeration ratio and presence of effluent recirculation are important to improve the alkalinity and pollutant removal in CWs. The influent pH (>10) was significantly decreased to 8.2–8.8 under the volumetric hydraulic loading rates of 0.105 and 0.21 d−1 in the CWs. Simultaneously, up to 91% of NH4+-N removal was achieved under the operation of a higher aeration ratio and effluent recirculation. Biological nitrogen transformations accounted for 94% of the consumption of alkalinity in the CWs. The significant enrichment of δ15N-NH4+ in the effluent (47–58‰) strongly supports the occurrence of microbial transformations for NH4+-N removal. However, relatively lower enrichment factors of δ15N-NH4+ (−1.8‰ to −11.6‰) compared to the values reported in previous studies reflected the inhibition effect of the high pH alkaline environment on nitrifiers in these CWs.

Benzene, toluene and xylene (BTX) belong to an important group of aromatic volatile organic compounds (VOCs) that are usually emitted from various sources. BTX play a vital role in the tropospheric chemistry as well as pose health hazard to human beings. Thus, an investigation of ambient benzene, toluene and xylene (BTX) was conducted at urban and rural sites of Gorakhpur for a span of one year in order to ascertain the contamination levels. The sampling of BTX was performed by using a low-flow SKC Model 220 sampling pump equipped with activated coconut shell charcoal tubes with a flow rate of 250 ml/min for 20–24 h. The analysis was in accordance with NIOSH method 1501. The efficiency of pump was checked weekly using regulated rotameters with an accuracy of ±1%. The samples were extracted with CS₂ with occasional agitation and analyzed by GC-FID. The total BTX concentration ranged from 3.4 μg m⁻³ to 45.4 μg m⁻³ with mean value 30.95 μg m⁻³ and median 24.8 μg m⁻³. The mean concentration of total BTX was maximum during winter (39.3 μg m⁻³), followed by summer (28.4 μg m⁻³) and monsoon season (25.1 μg m⁻³). The mean concentration of BTX at urban site (11.8 μg m⁻³) was higher than that at rural site (8.8 μg m⁻³). At both the sites, T/B and X/B ratios were highest in monsoon and lowest in winters. Toluene against benzene plot shows R² value of 0.96 and 0.49 at urban and rural sites respectively. Higher R² value at urban site clearly indicates similar sources of emission for benzene and toluene. At both the sites, the estimated integrated lifetime cancer risk (ILTCR) for benzene exceeded the threshold value of 1E-06 whereas the individual hazard quotients (HQ) for BTX did not exceed unity at any of the sites.