The EU Directive on the promotion of the use of biofuels or other renewable fuels for transport (CO M 2003/30/EC) states that the proportion of biofuels in 2010 must make 5.75% of non-renewable fuels, but in 2020 - 10%. The only way for Latvia to fulfil the Directive 2003/30/EC is to use winter rye because it is one of the oldest cereals grown in Latvia, and Latvia has a long-lasting experience in growing it. A field trial of winter rye (Secale cereale L.) was arranged in the Agricultural Science Centre of Latgale from 2005 to 2007. The influence of four winter rye varieties and five fertilizer rates on grain yield and starch content was researched for three years. The year (factor A), the varieties (factor B), the fertilizer rates (factor C), and the interaction of factors AB, AC , BC, and ABC significantly influenced the yield of grain and the starch content with 95% probability. It is recommended to use good quality grain with high starch content and low protein content for bioethanol production. The varieties of winter rye 'Kaupo', 'Amilo', and 'Walet' are the most suitable for ethanol production in Latgale region.

Grain after enzymatic treatment, which is a starch-containing raw material, is used for ethanol production. Bioethanol production in Latvia began in 2006. Extraction of biofuels is a clean process, because the byproduct is used in various sectors of the economy. The bioethanol in Latvia was derived primarily from winter wheat, winter rye, and winter triticale. The objective of the research is to determine the different nitrogen fertiliser rates required for winter cereal crop yields and bioethanol outcome. The trials were carried out from 2005 to 2008 in Agricultural Science Centre of Latgale (Latvia). The method (ethanol outcome) is based on fermentation of saccharified cereal samples by yeast Saccharomyces cerevisiae followed by the calculation of ethanol outcome and speed of fermentation. The highest starch content was in winter wheat and winter triticale grain, but the lowest - in winter rye grains. A close negative correlation (p is less than 0.05) was found for winter triticale and winter wheat between the ethanol outcome and thousand grain weight. Production of bioethanol from rye starch content is used with full utilisation of grain. The winter wheat has the largest ethanol outcome from one hectare.