The aim of the study was to find out the differences of blood biochemical and morphological parameters in cows with and without retained foetal membranes. Sixty cows of different age of Latvian blackand- white breed from two Joint Stock Companies were used in the research that was divided into two groups: control group of 15 cows, and 45 cows with retained foetal membranes. Research was done from September 2007 to January 2009. In haematological parameters on the second day postpartum, control group cows had increased leukocyte count on the account of mature neutrophils, but in the group of cows with retained foetal membranes leukocytes were within the range of normal values. The difference of the above mentioned parameters between both of the groups was significant (p is less than 0.05). At the same time, in cows of both investigated groups the number of band neutrophils was slightly increased (p is greater than 0.05). Haematological analyses showed leukocytes shift to the left and lymphopenia with a tendency to get normal within six weeks postpartum in both cow groups. On the third week postpartum, in cows with retained foetal membranes the band neutrophil count increased significantly (p is less than 0.05) indicating that the inflammation process has flared up. Blood for biochemical examination was sampled from cows within 48 hours postpartum in order to evaluate the functional condition of liver in milking cows. A significantly higher (p is less than 0.05) total and direct reacting bilirubin was observed in cows with retained foetal membranes, although it was within the range of normal values.

The objective of the study was to examine how the circumstances of different bull daughters – Latvian Brown cow - group productivity levels correlate with the blood biochemical composition indices in Latvian conditions. The daughters of seven bulls, selected for our research trial, were kept in the same barn and under rearing conditions. Their blood samples were taken in a single sampling activity and analyzed for blood serum biochemical averages. Also Cu, Fe, Zn, Mn and Mg were detected in blood serum and the values found were compared with values fixed in regulatory documents. There were changes in Cu levels (daughter groups of four bulls), in Zn levels (one group); Mn level was lower in all groups and Mg level was higher in 6 groups. The altered biochemical parameters of blood were assessed from the pathogenetical point of view and compared with productivity averages in the progeny groups. The correlation factors suggested that there exist a strong positive correlation (p is less than 0.05) among a number of indicators, such as aspartate aminotransferase (ASAT) and protein r = 0.823; ASAT and somatic cell count (SCC), r = 0.737; blood protein and milk protein r = 0.903 - 0.917; albumins and protein r = 0.964; blood urea and milk protein r = 0.931 - 0.984 along with additional correlations. The correlation factors for the progeny group of each bull differed. The data obtained shows that the Latvian Brown cows crossed with other breeds produce cows with new genetic traits.

The preservation of local animal breeds is a topical theme in recent years. Latvian Brown (LB) and Latvian Blue (LZ) cow breeds are exhausting and must be preserved. It can be performed by the use of multiple ovulation and embryo transfer (MOET). The difficulties exist in choosing the gene-fond (GF) cows as donors caused by the small number of animals. Twenty-three cows were intended for donor cow’s role from different herds until September 2018, and twenty of them were accepted. Anamnesis, clinical examination, blood morphology (13 parameters) and biochemical indices (19 parameters) were analysed before MO induction. Three cows were rejected because of ovarian cysts or pyometra, negative energy balance (NEB) (glucose is less than 2.3 mmol LE−1 simultaneously with elevated ß-hydroxybutyric acid is greater than 1.4 mmol LE−1) and elevated (p is less than 0.05) number of leukocytes (28.20 x109 LE−1). More than 52.6% of cows had a repeated artificial insemination before the last parturition, and 5.3% of cows had lifeless offspring in the last parturition. The 1st and 2nd lactation cows were healthier than older cows (p is less than 0.05). The amount of albumins, cholesterol, triglycerides and Na, K, P, Cl, Mg was significantly different in donors with and without successfully obtained embryos (p is less than 0.05). In conclusion, not only acceptable clinical health but also the cow metabolic status is a decisive factor for success of MOET.

Neosporosis is an infection disease caused by a single celled parasite Neospora caninum that produces cysts in the host organism. The dogs are a definitive host of parasite; cows and other ruminants are intermediate hosts. It causes neuromuscular disorders in dogs and abortions, birth of weak calves and neonatal mortality in cattle. Disease occurs worldwide (Sweden, Germany, Spain, Australia, Canada, USA et al.). Our aim of this study was to clear up how many seropositive animals in two seropositive dairy herds (after bulk milk investigation) in Latvia there are, their relationship and possible changes of their biochemical data. The studies in 2006 between September and November were carried out. In two farms there were 14 seropositive animals (from 67). There was evidence of inheritance of neosporosis infection. No differences were recognized in cows' blood biochemical data between positive and negative animals.