Two studies were conducted: the first study evaluated the production potential of indigenous (Tswana) chickens under an extensive free-range management system in the Gaborone agricultural region of Botswanal; and the second study was a survey of 25 randomly selected, commercial small-scale broiler farms in the Southern region of Botswana. Data were obtained using a structured questionnaire, interviews and direct observations of the birds and their management in the two systems. Study 1 involved 85 farmers. Ten different common supplementary feeds fed to the free-range chickens together with soil samples from where the chickens roost were collected. The feeds were: sorghum grain (Sorghum bicolor), maize grain (Zea mays), jugo bean, also known as bambara groundnut (Vigna subterranea), tepary bean (Phaseolus acutifolius), millet grain (Pennisetum glaucum), melon seeds (Colocynthis citrullus [Citrullus lanatus]), Tswana water melon fruit (whole), sorghum milo, maize bran and sorghum beer residue (moroko). Mean adult body weight of the birds (n=713) was 2.2 and 2.0 kg for cocks and hens respectively. Average egg weight (n=188) was 48 g with an annual production of 34 eggs laid in 2 to 3 clutches. Production varied little among flocks. Sexual maturity was attained at about 6 months for females. The crude protein content of the feeds (as-fed basis) given as supplements was: maize grain 9.8%, millet 6.5%, sorghum 11.1%, tepary bean 18.5% and jugo bean 15.8%. All the feeds had a low level of calcium, for example 0.22% for melon seeds. The farmers kept the chickens for home consumption and occasional sales. In study 2, all the farmers practiced intensive housing on deep litter. The number and size of the poultry houses ranged from one to eight, with floor area of less than 100m2 to 1000m2. Numbers of chickens in each batch ranged from less than 1000 to 10000 with about 4 batches in a year. The broiler strains were Ross hybrid, Indian River and Cobb with 56% of the farmers keeping Ross hybrid. Various heating methods were used for brooding with more than 40% using gas as a source of heat. Vaccinations for either gumboro and/or Newcastle were used by 76% of the farmers. Feed was provided ad libitum in all instances.

The aim of the study was to determine and compare calcium and phosphorus content in pedal mass, visceral mass and shell of wild and bred trial groups’ snails (Helix pomatia). The trial was performed from May to September of 2011 at the Roman Snail Research Facility of the Research Institute of Biotechnology and Veterinary Medicine „Sigra”, of Latvia University of Agriculture (LLU). Snails were divided in 4 trial groups depending on the diets. Samples were collected from the local natural population of snails and experimental breeding farm in spring (May), summer (July) and autumn (September). Significant differences (p is less than 0.05) were found between the levels of the calcium and phosphorus content in the edible part (pedal mass) and visceral mass in wild snails and experimental trial groups.

Human milk provides infant with the required nutrients for growth and development. The aim of preliminary study was to determine macro- and micronutrients content in mature human milk among lactating women residing in Latvia and dietary habits affecting it. The study was carried out from November 2016 to February 2017. In total, 24 pooled diurnal milk samples were collected from mothers whose infants had reached the age of at least two months. Personal information of each participant was recorded, including mother’s age, weight and height parameters, sex and age of an infant, parity, breastfeeding pattern, milk expression method used for sampling. Eating habits were obtained using Monthly Food Frequency Questionnaire. Analysed elements were determined using inductively coupled plasma mass spectrometry (ICP‑MS Agilent 7700x, Japan). Concentrations ranged from 227.52 to 398.34 mg LE-1 for calcium, 58.56 – 256.38 mg LE-1 for sodium, 445.33 – 736.71 mg LE-1 for potassium, 25.73 – 49.52 mg LE-1 for magnesium, till 3.17 mg LE-1 for iron, 0.28 – 2.77 mg LE-1 for zinc, 0.06 – 0.43 mg LE-1 for copper, 2.00 – 44.00 µg LE-1 for manganese, 1.00 –10.00 µg LE-1 for chrome which is comparable to data obtained from other studies although chrome and manganese concentration was even higher than observed in literature. Cobalt and selenium content was below detection limit. Zinc content in human milk negatively correlated with baby’s age (p is less than 0.05). The content of majority of essential minerals in human milk was affected by mother’s dietary habits; however, more samples need to be analysed for representative conclusions.

The mean content of minerals in grains varies significantly among different parts of the world as it depends on the type of grain growing conditions and fertilizer application. In this study, the content of minerals in the wheat, oat and barley grains of the crop of the year 2006 from different regions of Latvia were investigated. The grain samples were analysed for K, Mg, Ca, Na, Cu, Mn, Fe, and Zn using Atomic Absorbtion Spectrometer, and for P using spectrophotometer. Phosphorus content in different types of grains varied from 3.10 to 5.65 g kgE-1, potassium - from 4.37 to 6.10 g kgE-1, calcium - from 0.40 to 1.40 g kgE-1, magnesium - from 1.20 to 1.68 g kgE-1, manganese – from 14.50 to 55.54 g kgE-1, copper - from 3.35 to 6.01 g kgE-1, zinc - from 18.72 to 31.84 g kgE-1, iron - from 42.39 to 120.40 g kgE-1, and sodium - from 32.34 to 55.52 g kgE-1. The greatest difference was found between the content of manganese and iron in barley, wheat and oat grains, but small difference was between the mean content of P, K, Zn, Cu, Mg, and Na.

The purpose of this study to compare milk calcium and phosphorus content of different breeds raised in Estonia. Based on obtained results, different factors and their magnitude of influence on the contents of calcium and phosphorus in milk were studied. On Polula Research Farm five trial groups were made up: Estonian Holstein breed (breeding value 115) (EHF-t), Estonian Holstein breed (breeding value 105...115) (EHF), Red-and-White Holstein breed (RHF), Estonian Red breed (EPK) and Estonian Native breed (EK). Milk samples were collected from five of each trial group twice a month during the year 2001. Calcium and phosphorus contents were determined by using IDF methods 36A:1992 and 42B:1990 in Dairy Laboratory of the Institute of Animal Science.