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Tskhovrebov. V., Stavropol State Agrarian Univ. (Russian Federation) | Kukushkina, V., Stavropol State Agrarian Univ. (Russian Federation) | Faizova, V., Stavropol State Agrarian Univ. (Russian Federation) | Kalugin, D., Stavropol State Agrarian Univ. (Russian Federation) | Nikiforova, A., Stavropol State Agrarian Univ. (Russian Federation)

Studies were conducted at the experimental station of the Stavropol State Agrarian University on leached chernozem, powerful, low-humus heavy loam on loess-like loam in 2017. The goal was to study the effect of the introduction of rocks rich in chemical composition (limestone-shell rock, apatite and phosphogypsum), both separately and jointly, on the microbial phase of the soil. The determination of the number of microorganisms was produced on dense nutrient medium by direct counting of colonies. It was found that the amount of ammonifiers under the control was 37 ml CFU gE−1 (colony-forming units), increasing 1.3–1.5 times with separate use and 2.5–3 times with the joint use of rocks. Similar changes were observed with respect to the number of nitrifiers and aerobic nitrogen fixers of the type Azotobacter. The number of cellulose-depleting microorganisms in the remineralization variants reached 220,00–230,00 CFU gE−1 compared to 115,00 CFU gE−1 under the control. With the introduction of separate rocks, there was a decrease in the occurrence of pathogens, while with a joint introduction they were not detected. The frequency of occurrence of toxin formers, such as Aspergillus and Penicillium, reached 100% at the control and decreased by 20–40% at the experimental variants. It was revealed that the introduction of shell limestone, apatite and phosphogypsum had an effect on the increase in the number of soil microbiota of various physiological groups. Among the fungal microflora, the number of pathogens and toxin formers decreases and the number of pathogen antagonists increases.

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