The most non-starter lactic acid bacteria (NSLAB) isolated from semi-hard cheeses are heterofermentative and handled as one of the reasons of cheese off-flavours and yield defect but at the same time majority of researches argued positive effect of NSLAB on cheese flavour formation and diversification due to rendered compounds of chemical reactions. The amount of NSLAB in cheese varies from 10 at the beginning of ripening to 105 cfu mlE-1 within 6-8 weeks. The aims of this paper were to establish diversity of NSLAB in commercial samples of Krievijas and Holandes cheeses, and to evaluate the effect of ripening temperatures on NSLAB in the trials of Krievijas cheese. A total of 12 commercial cheese samples from seven different Latvian manufacturers and trials from one cheese manufacturer were examined. The trials were ripened for 60 days at different temperatures - 6 deg C and 12 deg C. Serial dilutions of each cheese sample (1:1000 and 1:10 000) in saline were made. NSLAB were cultivated using MRS media. Strain identification was performed by the API 50 CHL system (BioMerieux, Marey l'Etoile, France). In commercial samples of Krievijas cheese dominance of L. curvatus was observed, simultaneously L.plantarum and L.paracasei subsp. paracasei were isolated. Whereas in Holandes cheese samples dominance of L.paracasei subsp. paracasei was noted and L.plantarum, L.curvatus, L.rhamnosus and L.acidophilus were isolated. In the trials ripened at different temperatures prevalence of L.curvatus was noted. Concentration of Lactobacillus spp. varied from 104 cfu mlE-1 on the first day of ripening and reached the highest concentration (106 cfu mlE-1) after 6 weeks of ripening.

Dairy ecosystems have been developing in thousands of years giving us wonderful collection of different types of cheese varieties. On the other hand, modern technology provokes the loss of variability (Adamberg et al., 2007). The only way to keep biological processes under control is to study physiology of microorganisms, and relationships between them systematically, especially non-starter lactic acid bacteria (NSLAB). The number of non-starter lactic acid bacteria increases during cheese ripening and may constitute a dominant population in the mature cheese. The NSLAB diversity, their metabolism and interactions with starter bacteria have an effect on the ripening process of the cheese. The aim of the present study was to investigate the diversity of Lactobacillus spp. during ripening of Krievijas cheese. The diversity of different profiles varied among the cheese samples matured at different temperatures, and the results indicated a correlation between NSLAB species and aroma development (Miķelsone et al., 2009). A higher metabolism of Lactobacillus curvatus at 12 deg C leads to faster depletion of nutrients and approaching the last two phases of microorganism growth curve. However, in each of the cheeses, the microflora had a tendency to be dominated by one Lactobacillus profile at the end of maturation.

The purpose of this study was to evaluate the effect of probiotics, herbals and buckwheat bran (Fagopyrum esculentum L.) on growth, profile of blood, gut microbiota, profile of fatty acid in meat and shedding of resistant Escherichia coli (E. coli) in piglets. A total of 44 piglets (Sus scrofa domesticus) from age of day 14 to 56 were divided into 4 groups. Control received basal diet (group C), basal diet + probiotics (group P), basal diet + 3% buckwheat bran (group PB) and basal diet + 1.5% herbals (group H). No effect was observed in growth in all groups. The count of Lactobacillus spp. increased (p is less than 0.05) in jejunum in group P. In the faeces, Enterobacteriaceae decreased in the group P (p is less than 0.05) of 35 days old piglets, but Enterobacteriaceae and E. coli decreased in the group H (p is less than 0.05) of 56 days old piglets. The prevalence of resistance to at least one antibiotic class was 66.7% before and 50% after the experiment in all groups. Multidrug resistance of E. coli was not observed in 14 days old piglets, but was observed in 50% and more in all of study groups of 56 days old piglets. The fatty acid composition of Longissimus thoracis muscle had higher levels of α-linolenic acid and palmitoleic acid (p is less than 0.05), but lower level of stearic acid (p is less than 0.05) in group P. In conclusion, probiotics and herbals improved gut microbiota, fatty acid profile and affected shedding of resistant E. coli, but not growth performance.

The diversity of non-starter lactic acid bacteria in raw, pasteurised milk and maturated Swiss type cheese was tested. The aim of the present study was to analyse the concentrations and representatives of non-starter lactic acid bacteria in raw milk and to evaluate the changes of their concentrations and representatives during pasteurisation of cheese milk and Swiss-type cheese production. The analysis carried out in the study showed a variation in the microbial composition and quantity of raw milk. The most frequently isolated lactobacilli were found at low level in raw milk (mean 27.5×104 CFU mLE-1) and the most frequently identified species were Lactobacillus brevis and Lactobacillus paracasei. The microflora of raw and pasteurised milk is similar analysing lactic acid bacteria representatives in the samples. Lactobacillus brevis and Lactobacillus paracasei were detected in the same samples in raw milk, pasteurised milk and maturated cheese. Our study showed that lactic acid bacteria concentration was quite low in pasteurised milk (1-12 CFU mLE-1), but they grow rapidly in cheese during ripening reaching 1.1-1.8*106 CFU gE-1. The present study has shown that NSLAB in Swiss-type cheese mainly derive from raw milk, and only a few strains survive the processing conditions and grow during ripening.

In this study we described the diversity of lactic acid bacteria and their representatives in raw and thermally treated milk, focusing on their potential in cheese production influencing cheese quality. The aim of the present study was to analyse the concentrations and representatives of lactic acid bacteria in raw milk and to detect the changes of lactic acid bacteria microflora during thermal treatment of cheese milk at a dairy processing plant. The analysis carried out in the study showed a seasonal variation in the microbial composition and quantity of raw milk. The most frequently isolated lactic acid bacteria: lactococci, lactobacilli, leuconostoc were found at low level in raw milk (mean 9.27×103 CFU mLE-1) and the most frequently identified species were Lactococcus lactis, Lactobacillus brevis and Lactobacillus fermentum. The microflora of raw and pasteurised milk is similar to the analysed lactic acid bacteria representatives in the samples. Interestingly, we found the same species in raw milk and pasteurised milk, for example, Lactobacillus brevis and Lactobacillus fermentum were detected in the same samples in raw milk and pasteurised milk. Our study showed that lactic acid bacteria concentration was quite low in pasteurised milk (0-76 CFU mLE-1), but they grow rapidly in cheese during ripening; therefore the definition of limits of the non–starter lactic acid bacteria colony forming units in milk should be reasonable for selection of appropriate raw milk quality for cheesemaking.