An important factor in the development of forest ecosystem is the ability to regenerate. Natural intensity of self thinning of a forest depends on the tree species and environmental conditions. Due to abiotic and biotic factors in a continuous forest tract, there appears a clearing, which, depending on the size, forms new growth conditions. Over time, the resulting new space is occupied by herbaceous and woody vegetation. Most often regeneration of a new forest depends on the size of the plot. The study was conducted in 2013 during the growing season in a typical broadleaf forest stand. During the study woody vegetation and projection coverage of herbaceous vegetation was registered in large clearings. Light conditions in the plots and under tree canopies, as well as soil parameters were ascertained. Based on the collected data, the view of the structure of woody vegetation, projection coverage of herbaceous vegetation, light conditions, temperature, soil moisture content and pH changes were obtained. In order to clarify the influence of microclimatic conditions on natural forest regeneration, the data on light and soil characteristics were analyzed. The aim of the study - was to determine the changes of woody and herbaceous vegetation in spruce stand clearings and to assess the impact of microclimate. During the study it was found out that in large plots dominated species demanding higher amount of light, while herbaceous vegetation was attributed to the third, fourth groups of aggressiveness. Naturally regenerated seedlings condition was mostly influenced by light conditions and soil moisture content.

The impact of light penetration into crown and the effect of rootstocks and distance between trees on photosynthetic behaviour were analysed. Apple cultivar ‘Auksis’ was grafted onto dwarfing rootstock P60 and super-dwarfing rootstock P22 and planted at different distances (3 × 1 m, 3 × 0.75 m and 3 × 0.5 m). Nitrogen balance index and photochemical reflectance index were measured at two heights: 0.8 m above ground inside the canopy and 1.5 m above ground outside the canopy; specific leaf area, fresh and dry weight were evaluated from all canopy. The significantly positive effect of dwarfing rootstock P60 on all tested indices was observed comparing to P22 rootstock. Increasing density between trees lead to a decreased specific leaf area and increased nitrogen balance index. The dry and fresh weight ratio and photochemical reflectance index were also significantly affected, but no general tendency was identified. Photochemical reflectance index was not affected by light penetration into canopy, but the nitrogen balance index significantly decreased in the upper part of the canopy. Summarizing, it can be stated that decreasing light penetration into the crown results in an increase in the specific leaf area and photochemical reflectance index, and leads to a decrease in dry and fresh weight ratio and nitrogen balance index.

The regeneration of European hornbeam (Carpinus betulus L.) has been assessed in the National Gene Reserve Forest near Lukne in the south-western part of Latvia, which is the northernmost point of hornbeam distribution in Europe. In the studied stand, six sampling plots and four transects were established to assess stand structure and the effect of irradiation parameters on stand regeneration. Successful regeneration of hornbeam was shown by its presence in all height and diameter classes. Nevertheless, in the smallest height and diameter classes, an increased abundance of other shade tolerant species (maple (Acer platanoides L.) and lime (Tilia cordata Mill.) was observed. Norway spruce (Picea abies Karst.) mainly formed a scattered second forest floor and, apparently, competed with hornbeam. Light parameters, particularly, the diffuse radiation, had a significant effect (p=0.05) on the distribution and abundance of hornbeam understory. The threshold values of irradiation parameters for hornbeam were notably higher compared to other species. The number and height of hornbeam saplings correlated tightly with the amount of available light. Nevertheless, under sufficient light conditions hornbeam showed good vitality, thus suggesting suitability of growing conditions.