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Piginka, I., Latvia Univ. of Agriculture, Jelgava (Latvia);Institute of Food Safety, Animal Health and Environment BIOR, Riga (Latvia) | Birgele, E., Latvia Univ. of Agriculture, Jelgava (Latvia)

The aim of the present study was to investigate the histological status of lymph nodes lnn. inguinales superficiales sinister et dexter, lnn. mesenterici cranial, and lnn. tracheobronchales for porcine circovirus-2 (PCV2) seropositive and seronegative pigs from two Latvian farms. The research was carried out in 2009. Two Latvian farms were investigated – conditional farm A in Latgale, and farm B in Kurzeme. Pigs from farm A had never been vaccinated against PCV2, but on farm B the PCV2 vaccination programme had been stopped six months ago. Ten pigs, 5-15 weeks old, were selected from each farm for investigation. Generally, four lymph nodes were taken from each animal. The blood serum of all 20 pigs was serologically tested for the PCV2 antibody of ELISA Synbiotics, Serelisa PCV2 Ab Mono Blocking kits. Four structural parameters of a lymph node were detected on histological slides: visualization of follicle structure, lymphocyte depletion, amount of histiocytic cell infiltration, and amount of multinucleate giant cell infiltration and localization in the lymph node. Only in lymph nodes of PCV2 seropositive pigs multinucleate giant cells were detected, which is an important additional parameter for serological test and is suggestive of PCV2 infection. As a result, an interrelation between the amount of histiocytic cells and the follicle structure was detected in pigs' lymph nodes: the more histiocytic cells in the lymph node, the greater the loss of the lymphoid follicle structure.

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