Understanding the efficacy of organic trace minerals has been increasingly important over the past few years as a result of increased customer awareness of their benefits and the increase in the number of commercial products available to the customer. Organically bound trace minerals of interest in animal nutrition specifically include iron, zinc, manganese, copper, cobalt, and selenium. These elements have been shown to improve reproductive efficiency as measured by conception rate, alleviated calving, and reduced placenta expulsion rate. Additional benefits include improved growth performance of calves. The field trials in the period of 2005 – 2007 with Charolais breed beef cattle cows and calves verified efficacy of the premix PROTRACE G containing Fe, Zn, Cu, Mn, and Co chelates, Se-amino acid (selenomethionine), potassium iodide, and vitamins A, D3 and E. Average calving rate of experimental group cows fed diet with added premix was 20 – 30 min, placenta expulsion time - 28 – 31 min, but in control group fed only basal diet - 2 h 21 min and 1 h 30 min – 2 h 09 min (p is less than 0.05) respectively. Live weight gain of calves and heifers of the experimental group was on 35 – 39% higher (p is less than 0.05) than that in the control group.

The high toxicity, bioaccumulative and increased distribution of cadmium (Cd) in the environment, makes it the most dangerous to any biological system, including immune system in human and animals. The effect of dietary intake of Cd (8.25 mg per kg) on accumulation and distribution of this heavy metal in various tissues, and functional changes in organs of immunity (thymus, bursa of Fabricius, spleen) in 35-day-old broiler cockerels were investigated, using biochemical and immunological methods. Significant increases in the Cd concentration both in central immunocompetent organs (thymus, bursa of Fabricius) and peripheral (spleen) were established. Excessive tissue level of Cd induced the prooxidative effect of this heavy metal in the organs. It was manifested in an increase of cell membrane lipid peroxidation (the enhanced malondialdehyde (MDA) concentration) in immune system organs. The oxidative stress resulted in immunocompetent cell damage. The fall of vital dye absorptive ability of immunocyte indicated the increase in the injured cell number. This harmful effect is in accordance with T- and B (C3) – population prominent depletion, organ relative mass reduction, and growth retardation in chicks, and was established as a result of dietary Cd loading for 5 weeks of the experiment.

Ruminants produce a large amount of methane (CH4 ) and carbon dioxide (CO2 ) in their foregut. These gases cause greenhouse effect. There are a lot of studies about different feed additives which can reduce the production of greenhouse gases in ruminants. Prebiotics can also change the amount of bacteria in animal gastrointestinal tract and reduce the occurrence of diarrhoea. The aim of this study was to test whether the prebiotic inulin affects the production of CH4 and CO2 in calves’ rumen and whether it affects the bacteria count in the rumen fluid and bacterial overgrowth in intestines. We used the flour of Jerusalem artichoke (Helianthus tuberosus L.) containing 50% of inulin. Approximately fifty days old, Holstein Friesian crossbreed calves were used in this study. Eight were in the control group, 8 received 12 g of flour and 8 received 24 g per day. On the 28th and 56th day of the research, we measured the amount of CH4 and CO2 in calves’ rumen took rumen fluid samples for bacterial analysis and urine to measure the level of phenol and indican. We concluded that adding the flour of Jerusalem artichoke at doses 12 g and 24 g did not significantly impact the production of CH4 and CO2 in calves’ rumen, the prebiotic inulin may suppress the growth of anaerobic microorganisms in the rumen at concentration 12 g of inulin reaching 56th day of experiment. The amount of phenol and indican in calves’ morning urine did not correlate with the faecal consistency of calves.

A study was conducted to determine efficiency of a phytoadditive on pig growth processes and digestive tract microflora. The pigs of control group were fed without the phytoadditive. The feed of the trial group piglets contained 0.5% of the phytoadditive per tonne feed, for starter pigs and finished pigs - 0.2% per tonne feed. The study indicated that at the age of 170 days, pig mass in the trial group was 111.67+-1.22 kg on average, but in the control group - 101.79+-0.81 kg, which showed that pigs from the trial group had by 9.7% higher average mass than in the control group (p is less than 0.05). Average daily gain for the trial group was 0.777+-0.009 kg, which was by 12 % more than for the control group pigs (p is less than 0.05). Feed conversion in the trial group was 2.928 kg, but in the control group - 3.129 kg, which was by 6.4% higher than in the trial group. Gastric microflora analyses showed that use of phytoadditive reduced mould colony forming units (CFU) amount in the trial group decreased by 24 times. Duodenum microflora analyses showed that use of phytoadditive reduced mould CFU amount by 25%, yeast CFU amount by 34%, Escherichia coli mesophilic and termophilic forms CFU by 16.3% compared to the control. A lactic acid bacterium CFU in the trial group was 2.5 times higher compared to the control. Rectum microflora analyses showed that use of phytoadditive reduced mould CFU amount by 31.6%, yeast CFU amount - by 62%, Escherichia coli mesophilic and termophilic forms by 57 % and 15.6 % respectively. Lactic acid bacteria CFU amount in 1 g of sample in the trial group increased by 5.1 times.