Raw milk cheeses are known to have more intense and strong flavour and different texture due to natural microbiota and enzymes. Nevertheless, there are concerns about safety of these products. For microbial inactivation heat treatment of milk is used, but it can adversely affect the flavour, taste and texture of the product. Therefore, applying non-thermal technology such as high pressure processing is attracting alternative. The aim of this study was to examine the effects of high pressure treatment of cow`s milk at a wide range of pressures (400–600 MPa) on milk rennet coagulation time, curd firmness and curd yield. Processed milk samples were subjected to enzymatic coagulation using commercial rennet to determine rennet coagulation time, yield of coagulum and curd firmness. High pressure processing insignificantly influences coagulation properties of whole milk. However, the magnitude of changes depended on applied pressure. Rennet coagulation time and curd yield were significantly different (p is less than 0.05) among the pressure treated milk samples. The higher firmness of the curd form pressurized milk than that of raw or pasteurized milk, evaluated positively. The main effects of high pressure treatment in milk appeared to involve dissociation of casein micelles from the colloidal to the soluble phase. This study suggests that high pressure treatments of milk at 500 MPa or 550 MPa for 15 min may be beneficial for improving the coagulation properties of milk. These positive effects indicated that high pressure processing may have potential for new cheese varieties development.

Fresh meat is a highly perishable product due to its biological composition as it serves as an ideal environment for the growth and propagation of microorganisms and common food-borne pathogens. High pressure processing (HPP) is a cold pasteurization treatment to extend shelf-life while preserving the sensory and nutritional characteristics of the product. The aim of this research was to evaluate the effects of HPP on the fresh porcine Musculus longissimus lumborum microbial load and related physical properties (pH, water activity aw, and moisture content). Vacuum packed meat samples were treated at 50, 100, 200, 300, 400, and 500 MPa for 1, 5, and 15 min in a high-pressure processor ISO-Lab S-FL-100-250-09-W (Stansted Fluid Power Ltd., UK). Pressure treatment above 300 MPa resulted in a significant (p is less than 0.05) decrease of total plate count. However, the studied pressurizing time had no significant effect on microbial lethality at the same pressure applied. Other important parameters such as water activity, moisture, and pH were determined as they directly affect microorganism growth and resistance to pressure. A slight increase in pork pH was observed with increased pressure. No significant changes in water activity and moisture content were observed as a result of high pressure treatment. For future researches it would be important to evaluate the dynamics of microbial growth during storing as part of cells after pressure treatment are injured and not eliminated immediately; therefore, microbial count may further decrease during cold storage.

High-pressure processing (HPP) is typically used for the microorganism inactivation, which provides safety and prolonged shelf life of meat and meat products. However, for consumers along with safety, it is important to have good sensory properties, which is a combination of tender and juicy meat with an intense meat flavour. These attributes may change because of the high pressure processing; therefore, the aim of the study was to evaluate the effect of HPP on sensory and physical attributes of pork upon processing at 300 and 600 MPa at room temperature for 1 and 15 min. After HPP the processed pork samples were cooked within the package in a water bath. Colour of cooked pork did not differ among samples. Moisture content of samples decreased with the increased processing time. Sensory evaluation revealed that HPP treatment did not influence the colour and flavour of cooked pork irrespective of treatment parameters applied in the current study. The panellists indicated that increased pressure made pork samples drier and tougher, thus changing such sensory attributes as juiciness and chewiness, which are important for meat palatability. The correlation found between chewiness determined by sensory analysis and toughness determined by Warner-Bratzler shear device suggested this instrumental method as a better tool when compared to the instrumental texture profile analysis (TPA).

The aim of the study was to assess the ability of pathogens metabolic repair from injury within 10 days of refrigerated storage of milk after high pressure treatment. Two pathogenic strains – Listeria monocytogenes ATCC 7644 (LM) and Escherichia coli ATCC 25922 (EC) were inoculated in ultrahigh-temperature treated (UHT) milk at concentration of about 107 CFU mLE-1 and treated at 400, 500, 550, and 600 MPa for 15 min with inlet temperatures 20 °C, and then stored at 4 ± 2 °C to evaluate survival and growth of pathogens. By increasing the applied pressure, an increased rate of the pathogens’ inactivation was achieved. After 10 days of storage, milk treated at 400 MPa showed growth over 3.5 log CFU mLE-1 of L. monocytogenes and 1.7 log CFU mLE-1 of E. coli. In 550 MPa and 600 MPa treated milk samples after 8 and 10 days of storage colony formation occurred (3 CFU mLE-1 (550 LM) and 2 CFU mLE-1 (550 EC, 600 LM and 600 EC)). Although high pressure treatment is effective method for reducing of pathogenic bacteria, the metabolic repair from injury of bacterial cells in milk during storage should be considered.

The aim of this review was to summarize available bibliography on the possible applications of high pressure processing in dairy industry, the effect of this non-thermal treatment on bacterial microflora and milk constituents. Traditional thermal treatments applied to milk processing lower nutritional quality because many nutrients are heat labile. To overcome this problem, several non-thermal processing technologies including high hydrostatic pressure (HHP) processing have been developed. Pressures between 400 and 600 MPa inactivate microorganisms including food-borne pathogens; however, high pressure (HP) injured bacteria in milk during storage can recover. All enzymes are inactivated only at pressures of 800 MPa. During HHP the casein micelle size decreases, whey proteins are denaturated, the level of free fatty acids increases. These characteristics indicate that for better understanding and application of HPP in dairy industry research should be done to offer the numerous practical applications to produce microbially safe, minimally processed dairy products with improved performances, and to develop novel dairy products of high nutritional and sensory quality and increased shelf life.