Rhododendron spp. plants were surveyed for Phytophthora infection in Lithuania during 2010 – 2016. This study aims to identify Phytophthora genus pathogen which infects rhododendrons in Lithuania. Samples were taken from young sick plants with visible infection symptoms. Soil sampling was performed from the rhizosphere of sick plants. DNA from soil and plant was tested for the presence of Phytophthora genus pathogens. Data showed positive results of Phytophthora genus specific probe during real-time PCR. All tested diseased leaves and soil samples have indicated Phytophthora sp. infection during Alert-LF® Phytophthora spp. analysis. The extracted DNA concentrations were not very high for Phytophthora species identification, but in most cases, it was high enough for further researches.

In dairy cows shedding of Coxiella burnetii in milk can be persistent, sporadic to absent. Persistent heavy shedder cows are mostly highly-seropositive. Serological response due to C. burnetii antigenic phase demonstrates acute or chronic infection (serological response to phase II (PhII) or phase I (PhI) antigen, respectively). The aim of this study was to detect dynamics of C. burnetii DNA in milk and phase-specific serological response. In this study, the sera samples from 46 animals and milk samples from 34 were collected initially. Milk and sera samples from 36 animals were collected repeatedly. Samples were collected in five herds with previous history of C. burnetii infection from different parishes in Latvia – in 2017 and 2018. Milk samples were tested by detection of C. burnetii DNA by realtime PCR amplification using ‘ADIAVET™ COX REALTIME’ (ADIAGENE). Sera were tested by ‘VetLine Coxiella Phase1 and Phase2 ELISA’ (NOVATEC). Three cows (9%) in the first sampling and five (14%) in second sampling demonstrated shedding of C. burnetii DNA in milk . Six cows (13%) in the first sampling and eight (22%) in second sampling demonstrated positive serological response to PhI. Three cows (7%) in the first sampling and one (3%) in second sampling demonstrated a questionable serological response to PhI. Two cows (6%) in the second sampling demonstrated a questionable serological response to PhII . In herds with previous history of C. burnetii infection the number of animals demonstrating PhI positve serological response increases significantly (p0.05) during six months. In cows demonstrating positive serological response to PhI it continues and mostly remains unchanged during six months.

Avian mycoplasmosis have been considered a severe problem in poultry diseases; Mycoplasma gallisepticum being one of the most important. This study was conducted in Joint-Stock Company Balticovo, Latvia, to determine the prevalence of M. gallisepticum infection in hen’s flocks in the farm. A total of 904 serum and 335 swab samples from non-vaccinated birds against M. gallisepticum from 65 chicken flocks of different age, from day 1 to 75 weeks old, were all tested. The commercially available enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) tests were used. Results revealed that the seropositive flock rate, based on ELISA tests and, according to ratio that represents the extent to which a light source effectively stimulates the rods: S/P ratios and antibodies titer higher 1.076 were 22/904 (2.43%) and 4/904 (0.44%), respectively, while PCR-positive flock rates were not confirmed. Seroprevalence of M. gallisepticum in commercial layer flock in Latvia was more common seen in birds from 17 to 30 weeks of age.

Indexing, the ELISA test, Spiroplasma citri culturing, nucellar selection and shoot tip grafting are procedures used in Corsica to maintain virus-free budwood. Corsica has managed to remain free of the major diseases of citrus: Tristeza, Phoma tracheiphila (mal secco), Spiroplasma citri (stubborn) greening and Xanthomonas citri (citrus canker)