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Determination of tryptophan metabolites in porcine adipose tissue: a preliminary report
2006
Zamaratskaia, G. | Jastrebova, J.
Tryptophan is a precursor for the production of skatole and indole in the large intestine of pigs. Accumulation of these compounds in adipose tissue causes on objectionable odour in meat from entire male pigs. Potential market impact illustrates the importance in developing an accurate method of determining skatole and indole content in adipose tissue. In the present study, a method is described for determination of skatole and indole concentrations by liquid chromatography combined with mass spectrometry (LC-MS) using atmospheric pressure chemical ionization (APCI). The optimization of chromatographic and APCI conditions is reported. This method showed excellent linearity over the concentration range tested (from 10 to 1000 ng mlE-1 of liquefied fat for both skatole and indole).
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