The objective of this study was to determine the effects of topical 1% atropine sulphate and systemic atropine sulphate on intraocular pressure (IOP) and horizontal pupil diameter (HPD) in dog’s eyes. Ten adult dogs for each treatment were used in this study. Dogs of different age, breed and sex were examined clinically and ophthalmologicaly. All animals were clinically and ophthalmologically healthy. One drop of topical 1% atropine sulphate was used in ten dogs unilaterally, with the contralateral eye acting as a control. IOP and HPD were measured every 5 minutes. In ten dogs systemic atropine sulphate were used intramuscularly (IM) with IOP and HPD measured every 5 minutes. In both study phases IOP and HPD were measured over a total duration of 60 minutes. After unilateral application of topical atropine, IOP increased significantly in the treated eye. A maximum average IOP of 20.3 mmHg in the treated eye was observed 20 minutes after treatment. Maximal pupil dilatation in the treated eye was observed 35 min after treatment. Measurements made after systemic atropine showed an IOP increase in both eyes, showing maximum average IOP increase 25 minutes post-treatment. Maximum average values of HPD were obtained 25 minutes after treatment. The HPD started to decrease 30 minutes after treatment but it was still significantly higher than before treatment (P is less than 0.05). Because of atropine sulphate’s ability to cause significant increase in IOP, it should not be used for diagnosis and treatment of glaucomatous eyes.

The influence of solvent, storage time and temperature on the stability of the mycotoxin sterigmatocystin (STC) was investigated. STC calibrants (1.0 μg mlE-1) in acetonitrile, methanol and mixture of acetonitrile and methanol (50:50, volume/volume) were stored in dark glass bottles at (– 25), 4 and 25 °C for up to 8 weeks. Samples were analysed by high performance liquid chromatography (HPLC) with ultraviolet (UV) detection. Obtained results indicate that more suitable solvents for storage of STC standard solutions at (–25 °C) are acetonitrile and mixture of acetonitrile and methanol (50:50, v/v), but not longer than for one week; for storage at 4 °C more suitable is mix of acetonitrile and methanol, but not longer than for one week.

Annual judging of dairy products including sensory evaluation of local cheese-maker' cheeses proved that the quality of Latvian classical cheeses is various. In fact, in last three competitions three different manufacturers gained the first place in category of Krievijas and Holandes types' cheeses. It indicates instability of Latvian cheese quality. Two types of semi-hard cheeses (Krievijas and Holandes) were selected for testing the quality of Latvian semi-hard cheeses in the beginning of 2008. Krievijas type cheese was selected from three different manufacturers, but Holandes type cheese - from five different manufacturers. Sensory properties such as flavour, structure and colour were measured using line scale method. Statistical analysis of obtained data showed differences between such assessed properties as colour (yellowness), texture (firmness), holes, flavour (acidity and bitterness) in Krievijas type cheese, and colour (yellowness), texture (firmness), flavour (saltiness) in Holandes type cheese.

Processing and cooking methods can greatly affect the nutritional value of fruits and vegetables including potatoes. In human nutrition potatoes before consumption are always prepared by several cooking methods and are one of the central components of warm meals. During treatment processes considerable changes in nutritional composition and nutritional quality of the product are induced. Hence the aim of the study was to determine the amount of basic nutrients (carbohydrate, fat and protein) as well as essential amino acids in five Latvian potato varieties prepared by the following heat treatment methods: baking in oven, shallow frying and deep fat frying and to compare the obtained results with recommended daily intake (RDI). Achieved results show significant differences in total carbohydrate amount within potato varieties (p=0.007) while within the type of heat treatments were no significance (p=0.065). Changes in fat content between heat treatment processes were considerable (p=0.000) whereas significance were discovered between control – pan fried, control – deep fat fried, baked in oven – pan fried, baked in oven – deep fat fried as well as pan fried – deep fat fried samples while no significance found among potato varieties (p=0.391). In protein amount neither within potato varieties nor between heat treatment processes significance was discovered, but the amount of essential amino acids significantly differs per each potato variety and type of heat treatment processes. Results on RDI per each nutrient group (fat, carbohydrates and protein) show significance on % RDI per each basic nutrient.

Smallholder livestock farmers face challenges such as lack of appropriate fora and networks which can motivate and influence the adoption of breeding strategies in West Africa. Efforts to ensure participation of livestock owners in breeding programs such as performance recording, the use of village herd stocks for breeding programs have been documented as has the creation of livestock producer organizations to enhance effective participation of farmers in breeding programs. The study was conducted in southern Mali where livestock such as the N’dama cattle are endemic. We explored baseline characteristics from a household and community survey to determine the potential effects of individual, household and network characteristics on the knowledge and use of livestock breeding strategies. We assume that interaction amongst stakeholders results in transfer of knowledge between stakeholders. Results from the stakeholder analysis of the types of stakeholders present at the site level were used in the Probit and Seemingly Unrelated Regression (SUR) models. Farmers’ organisations were commonly found at the village level. However, despite the presence of these organizations across sites, they do not interact with the technical organisations as often as they should hence a lower incidence of use of livestock breeding technologies across sites. Results from the models confirm the importance of networks as key determinants to improve the adoption of breeding technologies.

Three spectrophotometric methods were compared for the assessment of milk fat oxidation: the AOCS (American Oil Chemists' Society) 2-thiobarbituric acid (TBA) value direct method, the TBA method of Aristova, and the modified method for the estimation of total carbonyl compounds in oils of Endo et al. Two cow milk fat samples – a fresh fat, obtained from fresh milk, and a rancid fat, obtained from a rancid commercial butter by means of high-speed centrifugation (15.871 × g, 30 minutes at 40 deg C) – were analyzed several times in a 22-day period after sample preparation. A positive linear correlation between absorbance and storage time was established in all used methods. The method of Aristova is appropriate for exact determination of oxidation products. This method had rather good R2 value (0.85) of the fresh fat sample results; however, the coefficient of variation (45.78%) showed rather large deviations between parallel analyses. The methods of AOCS and Endo et al. can be used for the assessment of the dynamics of fat oxidation by reporting absorbance values from the assay directly. By these methods rather high R2 values (0.86, 0.93) of the fresh fat sample results were obtained; the coefficients of variation also were satisfactory (10.13%, 11.12%). The last two methods are cheaper and require smaller sample amounts (less than 0.2 g), while the method of AOCS is the most time-consuming method.

The pears (Pyrus communis) are one of most popular fruits that consumers willingly use as fresh produce. On the market place, consumer mainly pays attention to per's externals, size, colour, as well as to fruit taste, substantially influenced by degree of readiness, which in its turn has affect on the chemical composition of pears. The physical, chemical and sensory indices are significant when fruits have been used for fresh-cut fruit salad preparation. All tests have been carried out at Latvia State Institute of Fruit Growing, Dobele in year 2009-2010. The objective of this study was to evaluate the physical, chemical and organoleptical properties of winter cultivars of pears grown in Latvia. The object of the research was 13 cultivars of pears: 'Delta', 'Latgale', 'Tayushchaya', 'Talgarskaya', 'BP 8965', 'Elektra', 'Janvarskaya', 'Beta', 'Bere Kiyevskaya', 'Eckehard', 'Belorusskaya Pozdnaya', 'Erika', and 'Conference' and as a control commercial cultivar. The pears were analysed and their parameters were measured: average mass and diameter of fruits, flesh firmness, colour of flesh, titratable acid (TA) and soluble solids content (SSC) were determined. Sensory analyses were carried out by 9-point hedonic line scale method. Soluble solids content (degree Brix) of pear fruits grown in Latvia is within 9.6 - 14.5 degree Brix. The titratable acidity (TA) is insignificant (0.05-0.26%). The average mean mass of one fruit depending on a cultivar, is 174 g, the flesh firmness at the maturity stage is on average 10.7 to 26.5N. The fruits of cultivars 'Tayushchaya', 'Elktra', and 'Bere kiyevskaya' by their sensory indices were evaluated as the best.

Researching new remote sensing and data processing methods is very important subject in forestry. The objectives of research are to explore methods to determine single tree characteristics using LIDAR and adapt them for Latvian forest conditions. Different algorithms and mathematical relations for automatic calculation of tree species, coordinates, height and diameter at breast height are described. Within the project four different clustering methods for tree identification were evaluated. The first method's construction is based on reflection point count in certain height range. The second and third methods are searching for global and local maximums on height axis of LIDAR data collection. In the fourth method segmentation of aerial photography is done by using the user selected sample data. Tree tops were s discovered by searching similarly coloured regions. Field measurements were used for the calibration of LIDAR data and analysis. Sample plots were fitted in the study area with different species composition, age and density. The total number of measured trees in sample plots is 1844. Results show that height can be found mainly for I, II, III craft class trees with average error 2.5%. Stem diameter estimation error of pine is 28%, spruce 17%, birch 4.2% and second storey trees 5.4% using linear equations D = 0.6616*H + 4.6969 (for coniferous trees) and D = 0.7756*H + 3.7132 (for deciduous trees). Dividing trees in classes of coniferous and deciduous can be done by using near infra red photography. The total number of first storey trees identified by LIDAR is 91%, by aerial photographic method 94%.