In this study, the milk quality characteristics of Simmental (SIM) and Red-Holstein (RH) breeds raised in a private farm in Aydın were determined. As milk quality properties, the percentages of protein (MPC), lactose (MLC), non-fat dry matter (NFDMC), total dry matter (TDMC), casein (MCC) contents and also milk urea nitrogen (MUN), oleic acid (OA), beta-hydroxybutyric acid (BHBA) and somatic cell count (SCC) were determined. The averages of MPC, MLC, NFDMC, TDMC, MCC, MUN, OA, BHBA and Log10SCC belonging to RH and SIM breeds are 3.38 ± 0.021% and 3.40 ± 0.015%, 4.86 ± 0.028% and 4.81 ± 0.019%, 9.09 ± 0.037 and 9.09 ± 0.025, 11.18 ± 0.069 and 11.23 ± 0.048, 2.50 ± 0.020 and 2.44 ± 0.014, 12.07 ± 0.200 mg / dl and 12.28 ± 0.138 mg / dl, 0.258 ± 0.0095 g / 100 g and 0.255 ± 0.0065 g / 100 g, 0.284 ± 0.138 mmol / L and 0.269 ± 0.0093 mmol / L, and 5.417 ± 0.0173 (261216 cells / ml) and 5.401 ± 0.0118 (251768 cells / ml) were found, respectively. The breed did not differ significantly in milk quality characteristics, except for MCC. The lower SCC level in milk and the suitable level of MUN for both breeds shows that the factors such as maintenance-feeding-housing-herd management in the farm were optimal in this herd.