Herbal medicine also called botanical or phyto-medicine refers to using a plant's seeds, berries, roots, leaves, bark, or flowers for medicinal purposes. It is becoming more main stream as improvements in analysis and quality control along with advances in clinical research show the value of herbal medicine in the treating and preventing disease. This study was focused on a traditionally prepared and used a product consist of an oil complex (olive oil, Nigella sativa seed oil, rosemary oil); which were researched antimicrobial effect of the product alone, and each oil by disc diffusion methods. Gram-positive and -negative bacteria and yeasts were employed in the susceptibility tests. The antibiotics of imipenem and vancomycin were exploited as positive controls. While there wasn’t effect on Gram-negative bacteria, strong antimicrobial effects were observed on Gram-positive bacteria and yeasts. Nigella sativa seed oil was the most effective. There wasn’t antagonistic and/or synergetic effect for the oil complex product. Although essential oils may be assigned the same name, they may differ widely as antimicrobial agents due to the extraction method used, which may explain the distinctive antimicrobial results reported in different studies. This was the first study to investigate the antibacterial and antifungal effects of this herbal medicine.

The utilization of Nigella sativa L., commonly known as black cumin, in traditional and medicinal practices is well-documented, attributing to its wide-ranging biological activities. Given its significance, this study explores the efficacy of various seed priming treatments (control, distilled water, potassium humate, and gibberellic acid) under laboratory and field conditions to enhance the early growth stages of Nigella sativa L. Priming treatments aimed to improve germination rates, plant height, and other growth parameters, potentially translating into increased agricultural productivity. According to results plant height ranged from 52.38 to 58.91 cm, number of branches between 3.76 and 3.98 (branch plant-1). The number of capsules varied from 4.93 to 6.81 (capsule plant-1),  capsule seed weight was between 0.19 and 0.22 (gram capsule-1 ), and the thousand-seed weight ranged from 2.26 to 2.39 grams. The germination rate ranged from 78% to 93%, germination index 4.22-5.83 and mean germination time was observed from 4.27 to 4.82 days. Our findings reveal significant effects of priming on germination parameters and  plant height, this offering insights into the potential of these treatments to optimize crop growth. However, the transition of these benefits to field conditions, particularly yield-related parameters, appeared limited, suggesting the complex nature of growth enhancement strategies and their implications for agricultural practices.