Phthalate esters (PAEs) are synthetic compounds, commonly used as plasticizers and softeners in plastic material production, and they are recognized as endocrine-disrupting chemicals. This study was focused on monitoring the extent of PAEs migration in vacuum-packaged Kashar cheeses and plastic materials used in their packaging. A total of fifteen cheese samples were tested for PAEs, including benzyl butyl phthalate (BBP), dibutyl phthalate (DBP), diisononyl phthalate (DiNP), di-2-ethylhexyl phthalate (DEHP), and diisodecyl phthalate (DiDP). The quantification (LOQ) and detection (LOD) limits varied between 0.197 to 0.619 µg/mL and 0.059 to 0.185 µg/mL for all analytes, respectively. All phthalate ester concentrations in both of the cheese samples and their packaging materials were below the detectable level LOQ of the analytical method. FTIR spectra also confirmed that the packaging materials which consisted of polypropylene and polyethylene.