In this study, beta-lactam group antibiotic residues were investigated in raw cow's milk. For this purpose, a total of 86 raw milk samples were used as material. Samples were collected periodically from farms in various outlets and surrounding villages in the province of Sivas. Beta-lactam group antibiotic levels in raw cow's milk were determined by ELISA method with commercial test kit. According to the analysis results, beta-lactam levels were between 0.35-0.70 ng/mL in 64 (74.4%) and between 0.71-3.7 ng/mL in 22 samples (25.6%) determined of 86 raw cow milk samples. The residue levels detected in the samples are in accordance with the legal limits declared by the European Union (EU) commission and Turkish Food Codex Communique.

The objective of this study was to determine the prevalence of Listeria species, specifically Listeria monocytogenes, in raw milk, pasteurized milk, white cheese, and homemade cheese. A total of 200 food samples were collected and analyzed to examine the presence of Listeria spp. The EN ISO 11290-1 method was used for isolation of Listeria. API Listeria test kit was used for biochemically characterization. Listeria spp. were isolated in 25 of the 200 samples (12.5%). The largest number of Listeria spp. was detected in homemade cheese (24%), followed by raw milk (18%), and white cheese (8%). Listeria spp. were not isolated from the pasteurized milk. The most common species isolated were Listeria innocua (5.5%); the remaining Listeria isolates were Listeria ivanovi (3.5%), Listeria welshimeri (3%), and Listeria monocytogenes (0.5%). Listeria monocytogenes was detected in only raw milk.

The aim of this study was to determine the relationships of udder surface temperature (UST) with milk components, and somatic cell count (SCC) in dairy cows during the hot season. The study was carried out with 115 lactating dairy cows (Holstein, Simmental, and Holstein × Simmental) at a private dairy farm in Samsun, Turkey, with monthly visits. The UST was measured from the udder surface before cleaning and milking. At the same time, a portable cell counter and an automatic milk analyzer were used to determine the components and the SCC of the raw milk. The UST values of the cows changed significantly. Increased UST had an adverse effect on milk solids-non-fat (SNF), protein, lactose, and density levels. LogSCC values of cow groups with UST ≤35.0°C, 35.1-36.0°C, >36.0°C were found to be 4.475±0.0803, 4.774±0.1244, and 4.981±0.1491 respectively. The UST negatively correlated with SNF, protein, lactose, density, and freezing point, but positively correlated with LogSCC. As a result, performing UST measurements before milking may be beneficial to monitor udder health and to obtain high quality milk.

In this study, beta-lactam group antibiotic residues were investigated in raw cow's milk. For this purpose, a total of 86 raw milk samples were used as material. Samples were collected periodically from farms in various outlets and surrounding villages in the province of Sivas. Beta-lactam group antibiotic levels in raw cow's milk were determined by ELISA method with commercial test kit. According to the analysis results, beta-lactam levels were between 0.35-0.70 ng/mL in 64 (74.4%) and between 0.71-3.7 ng/mL in 22 samples (25.6%) determined of 86 raw cow milk samples. The residue levels detected in the samples are in accordance with the legal limits declared by the European Union (EU) commission and Turkish Food Codex Communique.

Escherichia coli is an emerging public health concern in most countries of the world. It is an important cause of food-borne human disease. The present study assessed the prevalence and determined the antibiotic resistance patterns of E. coli from raw milk marketed in Chittagong, Bangladesh. Of the raw milk marketed in Chittagong ~33(18%) of the 186 raw milk samples of it contains E. coli, indicator bacteria for any enteric pathogens. The mean viable count of total bacteria was 4.04×108 cfu/ml and the mean viable count of E. coli in the contaminated raw milk was 1.88×106 cfu/ml. E. coli from only six (18.2%) of the 33 positive samples yielded colourless colonies across the CT-SMAC, suggesting the probable presence of populations belonging to the serotype O157 and rest of the isolates 27 (81.82%) produced coloured colony on CT-SMAC considering the probable presence of populations belonging to the serotype non-O157. Growth of probable E. coli O157, as evidenced by the colourless colonies on CT-SMAC compared to coloured colonies from other bacteria. Confirmed isolates were further subjected to antimicrobial susceptibility test using the Agar disc diffusion technique. Antibiotics susceptibility profile showed that all the isolates in case of E. coli O157, penicillin (100%), tetracycline (100%), amoxicillin (83.33%) and erythromycin (83.33%) were the most resistant whereas ciprofloxacin (66.67%), gentamicin (50.0%), and streptomycin (50.0%) were the most sensitive antibiotics. In case of E.coli non-O157 susceptibility profile showed that chloramphenicol (40.74%), erythromycin (40.74%) and oxacillin (37.04%) were the most resistant whereas ciprofloxacin (70.37%), sulphamethoxazole/trimethoprim (S/T) (59.26%) and gentamycin (55.55%) were the most sensitive antibiotics. The antimicrobial resistance exhibited by E. coli O157and non-O157 strains in this study is an indication of possible antibiotic abuse.

The aim of this study was to determine the relationships of udder surface temperature (UST) with milk components, and somatic cell count (SCC) in dairy cows during the hot season. The study was carried out with 115 lactating dairy cows (Holstein, Simmental, and Holstein × Simmental) at a private dairy farm in Samsun, Turkey, with monthly visits. The UST was measured from the udder surface before cleaning and milking. At the same time, a portable cell counter and an automatic milk analyzer were used to determine the components and the SCC of the raw milk. The UST values of the cows changed significantly. Increased UST had an adverse effect on milk solids-non-fat (SNF), protein, lactose, and density levels. LogSCC values of cow groups with UST ≤35.0°C, 35.1-36.0°C, >36.0°C were found to be 4.475±0.0803, 4.774±0.1244, and 4.981±0.1491 respectively. The UST negatively correlated with SNF, protein, lactose, density, and freezing point, but positively correlated with LogSCC. As a result, performing UST measurements before milking may be beneficial to monitor udder health and to obtain high quality milk.

This study aimed to investigate the effect of pulsed UV light on the the preservation of the Şavak cheese. In this study, the samples of Savak cheese (average 2 cm) that were produced from raw milk were exposed to two-sided pulsed UV light. The microbial analysis (enterobacteria, psychrophilic aerob, lactic acid, sulphate reducing bacteria, S.aureus and yeast-fungus) and chemical analysis (acidity, pH and the number of thiobarbituric acid) of şavak cheese were performed during storage time (25 days) at 4°C. According to the results, spoilage was observed in the control group after ten days, Group 1 was the best in terms of microbiological quality. When experimental samples were compared to the control group, no statistically differences were observed in terms of TBA, acidity, and pH value.

Escherichia coli is an emerging public health concern in most countries of the world. It is an important cause of food-borne human disease. The present study assessed the prevalence and determined the antibiotic resistance patterns of E. coli from raw milk marketed in Chittagong, Bangladesh. Of the raw milk marketed in Chittagong ~33(18%) of the 186 raw milk samples of it contains E. coli, indicator bacteria for any enteric pathogens. The mean viable count of total bacteria was 4.04×108 cfu/ml and the mean viable count of E. coli in the contaminated raw milk was 1.88×106 cfu/ml. E. coli from only six (18.2%) of the 33 positive samples yielded colourless colonies across the CT-SMAC, suggesting the probable presence of populations belonging to the serotype O157 and rest of the isolates 27 (81.82%) produced coloured colony on CT-SMAC considering the probable presence of populations belonging to the serotype non-O157. Growth of probable E. coli O157, as evidenced by the colourless colonies on CT-SMAC compared to coloured colonies from other bacteria. Confirmed isolates were further subjected to antimicrobial susceptibility test using the Agar disc diffusion technique. Antibiotics susceptibility profile showed that all the isolates in case of E. coli O157, penicillin (100%), tetracycline (100%), amoxicillin (83.33%) and erythromycin (83.33%) were the most resistant whereas ciprofloxacin (66.67%), gentamicin (50.0%), and streptomycin (50.0%) were the most sensitive antibiotics. In case of E.coli non-O157 susceptibility profile showed that chloramphenicol (40.74%), erythromycin (40.74%) and oxacillin (37.04%) were the most resistant whereas ciprofloxacin (70.37%), sulphamethoxazole/trimethoprim (S/T) (59.26%) and gentamycin (55.55%) were the most sensitive antibiotics. The antimicrobial resistance exhibited by E. coli O157and non-O157 strains in this study is an indication of possible antibiotic abuse.