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The Effect of Antioxidants Added to Culture Medium on Blastocyst Development Rates Texto completo
2022
Mehmet Burakalp Yusuflu | Sakine Ülküm Çizmeci
The Effect of Antioxidants Added to Culture Medium on Blastocyst Development Rates Texto completo
2022
Mehmet Burakalp Yusuflu | Sakine Ülküm Çizmeci
The present study, it was aimed to determine the effect of antioxidants added to culture media on blastocyst development rates in in vitro embryo production. The material of the study consisted of oocytes collected from the ovaries taken from the slaughterhouse. Cumulus oocyte complexes (COC) were collected and classified under a stereomicroscope. Oocytes included in the study were subjected to maturation and fertilization stages. Probable zygotes were transferred to the culture (IVC) containing antioxidants (L-ergothionine 100 μM (n: 163), Vitamin E 100 μM (n: 151) Cysteamine 50 μM (n: 154) and were cultured in a tri gas incubator (Hera Cell- 6% O2%, 6%CO2, 88%N). Blastocyst rates and embryo quality were determined on the 6th and 7th days in culture medium. Differences in IVMFC stages were evaluated by chi-square test. 966 oocytes were collected from 162 ovaries collected from the slaughterhouse. It was determined that the number of oocytes per ovary was 5,96, and the number of A and B quality oocytes was 4.26. It was determined that 655 (94.93%) of a total of 690 oocytes undergoing in vitro maturation were mature. The cleavage rates of the groups were 83.44%; 80.79%; 79.87%, and 83.96%, respectively. 140 (21.37%) blastocysts were obtained from 655 oocytes taken into the culture stage and the blastocyst rates in the groups were 33.13%; 8.61%; 7.79%, and 32.62%, respectively. As a result of the study, it was determined that the rates of blastocysts in the L-ergothioneine added the group was similar to the control group, but the blastocyst rates decreased significantly in the cysteamine and Vitamin E added groups. It was thought that this decrease might have been affected by the dose of antioxidants or the adequacy of oocyte development
Mostrar más [+] Menos [-]Correlações fenotípicas em características fisicoquímicas do maracujazeiro-azedo Texto completo
2010
Morgado, Marcos Antônio Dell'Orto | Santos, Carlos Eduardo Magalhães dos | Linhales, Heloisa | Bruckne, Claudio Horst
A Digital Object Identifier (DOI) não foi disponibilizada pela revista. | O objetivo deste trabalho foi avaliar o grau de associação entre caracteres fisicoquímicas do maracujazeiro-azedo (Passiflora edulis), auxiliando assim os programas de melhoramento que utilizam a seleção indireta. Avaliou-se 1120 frutos colhidos em ensaio com 26 famílias de irmãos completos de maracujazeiro-azedo, nos quais mensuraram-se as seguintes características: número de frutos por planta (NF), produção estimada (PE), massa média do fruto (MF), massa média da casca (MC), massa média da polpa (MP), comprimento médio do fruto (CF), diâmetro médio do fruto (DF), espessura média da casca (EC), teor médio de sólidos solúveis totais (SST), acidez total titulável média (ATT), e coloração da polpa (CP). A correlação entre número de frutos por planta com produção estimada por planta apresenta magnitude maior (rf = 0.92**) que a correlação massa do fruto com produção estimada (rf = 0.54**), indicando a maior contribuição do número de frutos por planta para obtenção de famílias mais produtivas. A correlação de maior magnitude foi comprimento do fruto com massa do fruto, indicando que a seleção de plantas com frutos pesados podem ser feitas diretamente no campo, a partir do comprimento do fruto. | The objective of this study was to evaluate the degree of association between characters of Passiflora edulis thus supporting the breeding programs that use indirect selection. Was evaluated in 1120 harvested in test 26 full-sib families of passion fruit, in which measured the following characteristics: number of fruits por plant (NF), estimated production (EP), fruit weight (FW) ; average mass of the shell (MC), average mass of pulp (MP), the fruit length (FL), average fruit diameter (FD), rind thickness (EC), the average content of soluble solids (TSS) , average total acidity (TTA), and flesh color (CP). The correlation between number of fruits por plant with an estimated production por plant has greater magnitude (rf = 0.92 **) that the correlation of fruit mass with an estimated production (rf = 0.54 **), indicating a greater contribution of the number of fruits por plant to obtain more productive families. The correlation of greater magnitude was the length of the fruit with fruit mass, indicating that the selection of plants with heavy fruit can be made directly in the field, from the length of the fruit.
Mostrar más [+] Menos [-]Effect of Follicle Size and Follicle Stimulating Hormone Concentration on Nuclear Maturation of Bovine Oocytes In Vitro Texto completo
2015
Uğur Şen | Emre Şirin | Ercan Soyadan
The aim of the study was to investigate the effect of follicle size and follicle stimulating hormone (FSH) concentration on nuclear maturation of bovine oocytes in vitro. Follicles on bovine ovary were classified into 3 groups according to the diameter; small (
Mostrar más [+] Menos [-]Correlações fenotípicas em características fisicoquímicas do maracujazeiro-azedo Texto completo
2010
Dell'Orto Morgado, Marcos Antônio(Universidade Federal de Viçosa Departamento de Fitotecnia) | Magalhães dos Santos, Carlos Eduardo(Universidade Federal de Viçosa Departamento de Fitotecnia) | Linhales, Heloisa(Universidade Federal de Viçosa Departamento de Fitotecnia) | Horst Bruckner, Claudio(Universidade Federal de Viçosa Departamento de Fitotecnia)
The objective of this study was to evaluate the degree of association between characters of Passiflora edulis thus supporting the breeding programs that use indirect selection. Was evaluated in 1120 harvested in test 26 full-sib families of passion fruit, in which measured the following characteristics: number of fruits por plant (NF), estimated production (EP), fruit weight (FW) ; average mass of the shell (MC), average mass of pulp (MP), the fruit length (FL), average fruit diameter (FD), rind thickness (EC), the average content of soluble solids (TSS) , average total acidity (TTA), and flesh color (CP). The correlation between number of fruits por plant with an estimated production por plant has greater magnitude (rf = 0.92 **) that the correlation of fruit mass with an estimated production (rf = 0.54 **), indicating a greater contribution of the number of fruits por plant to obtain more productive families. The correlation of greater magnitude was the length of the fruit with fruit mass, indicating that the selection of plants with heavy fruit can be made directly in the field, from the length of the fruit. | O objetivo deste trabalho foi avaliar o grau de associação entre caracteres fisicoquímicas do maracujazeiro-azedo (Passiflora edulis), auxiliando assim os programas de melhoramento que utilizam a seleção indireta. Avaliou-se 1120 frutos colhidos em ensaio com 26 famílias de irmãos completos de maracujazeiro-azedo, nos quais mensuraram-se as seguintes características: número de frutos por planta (NF), produção estimada (PE), massa média do fruto (MF), massa média da casca (MC), massa média da polpa (MP), comprimento médio do fruto (CF), diâmetro médio do fruto (DF), espessura média da casca (EC), teor médio de sólidos solúveis totais (SST), acidez total titulável média (ATT), e coloração da polpa (CP). A correlação entre número de frutos por planta com produção estimada por planta apresenta magnitude maior (r f = 0.92**) que a correlação massa do fruto com produção estimada (r f = 0.54**), indicando a maior contribuição do número de frutos por planta para obtenção de famílias mais produtivas. A correlação de maior magnitude foi comprimento do fruto com massa do fruto, indicando que a seleção de plantas com frutos pesados podem ser feitas diretamente no campo, a partir do comprimento do fruto.
Mostrar más [+] Menos [-]The Effect of Antioxidants Added to Culture Medium on Blastocyst Development Rates Texto completo
2022
Mehmet Burakalp Yusuflu | Sakine Ülküm Çizmeci
The present study, it was aimed to determine the effect of antioxidants added to culture media on blastocyst development rates in in vitro embryo production. The material of the study consisted of oocytes collected from the ovaries taken from the slaughterhouse. Cumulus oocyte complexes (COC) were collected and classified under a stereomicroscope. Oocytes included in the study were subjected to maturation and fertilization stages. Probable zygotes were transferred to the culture (IVC) containing antioxidants (L-ergothionine 100 μM (n: 163), Vitamin E 100 μM (n: 151) Cysteamine 50 μM (n: 154) and were cultured in a tri gas incubator (Hera Cell- 6% O2%, 6%CO2, 88%N). Blastocyst rates and embryo quality were determined on the 6th and 7th days in culture medium. Differences in IVMFC stages were evaluated by chi-square test. 966 oocytes were collected from 162 ovaries collected from the slaughterhouse. It was determined that the number of oocytes per ovary was 5,96, and the number of A and B quality oocytes was 4.26. It was determined that 655 (94.93%) of a total of 690 oocytes undergoing in vitro maturation were mature. The cleavage rates of the groups were 83.44%; 80.79%; 79.87%, and 83.96%, respectively. 140 (21.37%) blastocysts were obtained from 655 oocytes taken into the culture stage and the blastocyst rates in the groups were 33.13%; 8.61%; 7.79%, and 32.62%, respectively. As a result of the study, it was determined that the rates of blastocysts in the L-ergothioneine added the group was similar to the control group, but the blastocyst rates decreased significantly in the cysteamine and Vitamin E added groups. It was thought that this decrease might have been affected by the dose of antioxidants or the adequacy of oocyte development
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