The identification of plants in which the bees forage is key in establishing bee farms and increasing honey production. In this study pollen analysis of honey samples from the southeastern part of Nigeria was carried out to ascertain their floral sources and ecological origin. The honey samples were acetolyzed and microscopically studied to determine the pollen types. A total of seventy-one pollen types belonging to forty-one families of plants were identified. The honey samples were dominated by pollen grains from the families of Arecaceae, Euphorbiaceae, Myrtaceae, Irvigiaceae, Fabaceae, Combretaceae/Melastomataceae, and phyllanthaceae. Some of the dominant pollen grain identified include Elaeis guineensis, Alchornea cordifolia, Hymenocardia acida, Ocimum gratissimum, Syzygium guineense, Nauclea latifolia and Afzelia africana. Out of the six samples studied Njikoka sample was monofloral having Mimosa pigra as predominant pollen while Ayamelum, Ekwusigo, Nsukka, Ezeagu, and Udenu samples are multifloral containing Elaeis guineensis, Phyllanthus sp., Piliostigma reticulatum, Irvingia sp., Alchornea cordifolia, and Lannea sp. as major secondary pollen. All the samples analyzed have Elaeis guineensis in common except Ezeagu, indicating that these plants are present in all five locations. These results can also be used as a tool in the geographical identification of Southeastern Nigeria honey from other geopolitical zones.