In this study, the changes in % yield, dry matter, ash, lipid, protein content, water activity, pH, total volatile basic nitrogen (TVB-N), total viable aerobic count (TVC), yeast and mold count, lactic acid bacteria (LAB), colour parameters and sensorial properties were analysed in rainbow trout (Oncorhynchus mykiss) exposed to either salt only or two different salt-sugar mixture treatments. For this purpose, three groups were formed. For the first, second and third group, fish samples were treated with only salt (S), salt and sugar blend (WS) and salt and brown sugar blend (BS), respectively. Then, the samples were vacuum packaged and stored at +4°C for 3 months. Overall, salt treatments, liquid smoking and cooking as well as storage generally caused remarkable changes in the parameters of interest. However, except for the sensory analysis, not a remarkable change was seen when the three groups were compared among themselves. The results of experienced panelists suggested that group BS samples had superior appearance, taste, odor and texture and therefore expected to be more preferred by the potential consumers.

Gelatin origin determination has been a crucial issue with respect to religion and health concerns. It is necessary to analyze the origin of gelatin with reliable methods to ensure not only consumer choices but also safety and legal requirements such as labeling. There are many analytical methods developed for detection and/or quantification of gelatin from different sources including bovine, porcine and piscine. These analytical methods can be divided into physicochemical, chromatographic, immunochemical, spectroscopic and molecular methods. Moreover, computational methods have been used in some cases consecutively to ensure sensitivity of the analytical methods. Every method has different advantages and limitations due to their own principles, applied food matrix and process conditions of material. The present review intends to give insight into novel analytical methods and perspectives that have been developed to differentiate porcine, bovine and piscine gelatins and to establish their authenticity. Almost every method can be succeeded in origin determination; however, it is a matter of sensitivity in that some researches fail to ensure sufficient differentiation.

Transposable elements (TEs) are the most abundant group of genomic elements in plants that can be found in genic or intergenic regions of their host genomes. Several stimuli such as biotic or abiotic stress have roles in either activating their transcription or transposition. Here the effect of the Panicum mosaic virus (PMV) and its satellite virus (SPMV) infection on the transposon transcription of the Brachypodium distachyon model plant was investigated. To evaluate the transcription activity of TEs, transcriptomic data of mock and virus inoculated plants were compared. Our results indicate that major components of TEs are retroelements in all RNA-seq libraries. The number of transcribed TEs detected in mock inoculated plants is higher than virus inoculated plants. In comparison with mock inoculated plants 13% of the TEs showed at least two folds alteration upon PMV infection and 21% upon PMV+SPMV infection. Rather than inoculation with PMV alone inoculation with PMV+SPMV together also increased various TE encoding transcripts expressions. MuDR-N78C_OS encoding transcript was strongly up-regulated against both PMV and PMV+SPMV infection. The synergism generated by PMV and SPMV together enhanced TE transcripts expressions than PMV alone. It was observed that viral infection induced the transcriptional activity of several transposons. The results suggest that increased expressions of TEs might have a role in response to biotic stress in B. distachyon. Identification of TEs which are taking part in stress can serve useful information for functional genomics and designing novel breeding strategies in developing stress resistance crops.

Bu çalışma, Trakya Bölgesinde iyi tarım uygulaması yapan ve yapmayan armut üreten tarım işletmelerini kapsamaktadır. Çalışmanın temel amacı, iyi tarım uygulaması yapan ve yapmayan işletmelerin girdi kullanım miktarlarının ve enerji kullanım etkinliğinin belirlenmesi ve ekonomik olarak karşılaştırılmasıdır. Kırklareli, Edirne ve Tekirdağ illerinde iyi tarım uygulaması yapan 16 armut üreticisinin tamamıyla, aynı sayıda iyi tarım uygulaması yapmayan armut üreticisi ile anket çalışması yapılmıştır. Değerlendirme sonuçlarına göre iyi tarım uygulayan işletmelerde toplam enerji girdisi 30.046,64 MJ, enerji çıktısı 36.000 MJ, enerji çıktı/girdi oranı 1,20, enerji verimliliği 0,50 kg/MJ, spesifik enerji 2,00 MJ/kg, enerji yoğunluğu 1,80 MJ/TL ve net enerji 5.953,36 MJ olarak hesaplanmıştır. İyi tarım uygulamayan işletmelerde toplam enerji girdisi 32.111,92 MJ, enerji çıktısı 33.600 MJ, enerji çıktı/girdi oranı 1,05, enerji verimliliği 0,44 kg/MJ, spesifik enerji 2,29 MJ/kg, enerji yoğunluğu 2,05 MJ/TL ve net enerji 1.488,08 MJ olarak hesaplanmıştır. Trakya Bölgesinde iyi tarım uygulaması yapan işletmelerde bir kg armut yetiştirmenin maliyeti 1,11 TL, iyi tarım uygulaması yapmayan işletmelerde 1,12 TL olarak belirlenmiştir. İyi tarım uygulaması yapan işletmelerde toplam masraflar 16.682,80 TL, gayri safi üretim değeri 24.250 TL, brüt kâr 14.938,10 TL, mutlak kâr 7.567,20 TL, nispi kâr 1,45 olarak hesaplanmıştır. İyi tarım uygulaması yapmayan işletmelerde toplam masraflar 15.652 TL, gayri safi üretim değeri 21.000 TL, brüt kâr 11.511,80 TL, mutlak kâr 5.348 TL, nispi kâr ise 1,34 olarak belirlenmiştir. Enerji kullanım etkinliği ve ekonomik analiz sonuçlarına göre iyi tarım uygulaması yapan işletmelerde armut yetiştiriciliğinin daha avantajlı olduğu belirlenmiştir.

This study was conducted to determine the water-sediment quality and trophic status changes of Cernek Lake located in the Kızılırmak Delta (one of the most important wetlands in Turkey) and protected as a Ramsar site. The main objective, was evaluated and examined the effects on trophic level of surface water that the layers of lake sediments can create. In the study, the periodic exchange on trophic level have been evaluated with Geographic Information Systems (GIS) by identifying existing water of lake and sediment quality of lake. Spatial analysis was realized for water and sediment quality parameters (pH, salinity, Secchi disc depth and chlorophyll-a, total phosphate and total organic carbon (TOC) concentrations). The results of field studies conducted at Cernek Lake for three seasons (2010-2011) showed that the sediments contain high phosphate (annual average 541 mg / kg PO4-P, dry weight) and high organic carbon content (annual average 22.4 G / kg TOC, Dry weight). During the summer, Carlson Index values relatively declined during this period compared to autumn (81 to 79), but the eutrophic structure of the lake is still found to be high character. Findings, showed that the contaminated lake sediment layer would be highly effective in trophic level of the lake therefore it has revealed the necessity of taking measures for eutrophication. According to the results of study, taking the medium and long term measures to eutrophication and implementation of the strategic action plan is required.

Collected blue-green algae (cyanobacteria) from freshwater sites throughout Tokat province and its outlying areas were isolated in laboratory environment and their morphological systematics were determined and also their species identifications were studied by molecular methods. Seven different species of blue-green algae collected from seven different sites were isolated by purifying in cultures in laboratory environment. DNA extractions were made from isolated cells and extracted DNAs were amplified by using PCR. Cyanobacteria specific primers were used to amplify 16S rRNA and phycocyanine gene regions using PCR. Phylogenetic identification of species were conducted by evaluation of obtained sequence analysis data by using computer software. According to species identification by sequence analysis, it was seen that molecular data supports morphological systematics.

This study was conducted to determine best evaluation method for hunted roe deer. For the research purposes, trophy papers of 192 roe deer hunted in hunting ground Srpska Crnja in 2009 and 2010 were used. A comparison of of trophy mass and CIC trophy value was carried out, as well as a comparison of mass and the trophies volume and a comparison of these two variables with an average length of the antlers and values of the aesthetic elements in order to determine whether the weight of antlers is good indicator of the value of the trophy. Where it was possible, a comparison of the commercial trophy value under the old and new system was carried out, in order to determine whether the same trophy costs the same in both systems, or which system is economically advantageous for the hunter. The results showed that the mass of trophies in most cases is a good indicator of the trophy value in CIC points. Thus, during hunting, focus should be on assessing the volume of antlers, since between volume and other indicators of the value of antlers there are stronger and more pronounced correlation than between the mass and these indicators.

Mercimek, Güneydoğu Anadolu Bölgesinde kuru tarım yapılan alanlarda, buğday veya arpa ile münavebe yapılarak yetiştirilmektedir. Bu çalışma, mercimek üretim alanlarında bulunan yabancı ot türlerinin belirlenmesi amacıyla, Şanlıurfa ilinde yürütülmüştür. Yabancı ot türlerinin yaygınlık ve yoğunluklarının belirlenmesi amacıyla, 2014 yılında 70 mercimek tarlasında survey yapılmıştır. İncelenen tarlalardaki en yaygın türler %64,29, 55,71, 51,43, 50,00, 45,71 ve 44,29 oranlarıyla; Sinapis arvensis L. (yabani hardal), Avena sterilis L. (yabani yulaf), Cephalaria syriaca (L.) Schrader (pelemir), Triticum aestivum L. (kendi gelen buğday), Galium aparine L. (dilkanatan) ve Vaccaria hispanica (Mill.) Rauschert (arap baklası) olarak sıralanmıştır. Çalışma yapılan tarlalarda yaygın ve yoğun olmamasına rağmen bazı geofit ve endemik türlere de rastlanılmıştır. Bölgede daha önce yapılan çalışmalarla kıyaslandığında, yabancı ot türleri ile bu türlerin yaygınlık ve yoğunluklarında önemli düzeyde değişiklikler olduğu belirlenmiştir. Bu değişikliklerin bölgede sulanan alanların artmasından sonra bitki deseninin ve üretim şeklinin değişmesiyle birlikte, herbisit kullanımı başta olmak üzere bazı tarımsal uygulamaların değişmesi gibi nedenlerle gelecekte daha da artacağı tahmin edilmektedir.

Even though dairy products play an important role in infant and human nutrition, they may also cause food borne diseases. Milk toxin AFM1 is one of the most important public health hazards. This toxin is produced by animals after consuming contaminated feed with AFB1 which is known for its carsinogenic effects and then excreted in milk. Same as AFB1, AFM1 is also carsinogenic, neurotoxic, nephrotoxic, hepatotoxic and immunsupressive for humans and cannot be destroyed by sterilization or pasteurization. For that reason, studies on cost effective and rapid methods for detection and detoxification of AFM1 in milk are quite popular among researchers. In this review, the worldwide prevalence of AFM1 in milk and milk products has been presented as well as the developments in techniques to detect and detoxify.

Bu çalışma, İstanbul ili Adalar İlçesinde (Büyükada, Heybeliada, Kınalıada, Burgazada) hobi bahçeleri ve peyzaj alanlarında yetiştirilen süs bitkilerinde Tomato spotted wilt virus (TSWV), Iris yellow spot virus (IYSV) ve Impatients necrotic spot virus (INSV) varlığının saptanması amacıyla, 2015 ile 2016 yılları arasında yürütülmüştür. Bölgede yapılan survey çalışmalarında simptomolojik olarak şüpheli süs bitkileri ile simptom göstermeyen süs bitkilerinden doku örnekleri alınmıştır. Toplanan 150 adet süs bitkisi örneği öncelikle Double Antibody Sandwich (DAS) ELISA yöntemi ile testlenmiş ve örneklerin hiçbirinde TSWV ve INSV infeksiyonu saptanmamıştır. ELISA testlerinde IYSV ile bulaşık olduğu saptanan örnekler RT-PCR çalışmalarında kullanılmıştır. IYSV-465c; IYSV-239f primer çifti ile yapılan RT-PCR çalışmalarında Pittosporum tobira ve Hydrangea macrophylla için 240 bp büyüklüğe sahip band gözlenmiştir. Böylece, IYSV’nin Adalar ilçesinde varlığı moleküler olarak da ortaya konulmuştur.