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Toxicity and Reproductive Parameters Impairment of Cypermethrin in Male Guinea Pig (Cavia porcellus)
2018
Bertin Narcisse Vemo | Augustave Kenfack | Ferdinand Ngoula | Edouard Akono Nantia | Claude Cedric Njieudeu Ngaleu | Arthénice Jemima Nounamo Guiekep | Astride Martine Megnimeza Tsambou | Ferry Nana Yidjeu | Chancel Patrick Nelo | Alexis Teguia
Cypermethrin is a large spectrum action insecticide, globally employed to control pests in agriculture and some human and domestic animals ectoparasites. This study aimed to evaluate its toxicity and reproduction impairment in male guinea pig. Forty adult male guinea pigs were divided into 4 groups and orally submitted to 0, 92, 137.5 and 275 mg/kg body weight/day for 90 days. The weight of the liver increased significantly, while that of kidneys decreased significantly in treated animals compared to controls. Serum concentrations of creatinine, urea, ALAT, ASAT, total cholesterol, prostatic acid phosphatase increased significantly, while the testicular total protein level decreased significantly in groups given the insecticide relatively to the control. The testes weight, libido, serum level of testosterone, mobility, sperm count and the percentage of spermatozoa with entire plasma membrane decreased significantly in animals exposed to cypermethrin with reference to controls. The percentages of abnormal spermatozoa increased significantly in animals submitted to 137.5 or 275 mg/kg body weight (bw) of cypermethrin compared to control ones. On the testis histological sections of pesticide-treated animals, immature germinal cells were observed in the lumen of seminiferous tubules. Cypermethrin was toxic in male guinea pig and damaged reproductive parameters.
Mostrar más [+] Menos [-]Hatay İli Yonca Üretim Alanlarında Bulunan Böcek Faunasının Tespiti ve Bazı Türlerin Popülasyon Yoğunlukları
2018
Kamuran Kaya
Bu çalışma 2009-2010 yıllarında Hatay ilinin, Antakya merkez ilçeye bağlı, Narlıca beldesinde bulunan iki farklı yonca tarlasında yürütülmüş olup, yonca bitkisinde bulunan fitofag türler, doğal düşman türleri ve bunların popülasyon yoğunluklarının belirlenmesi amaçlanmıştır. İlk yıl iki haftada bir, ikinci yıl haftalık olarak yapılan örneklemelerde, köşegenler doğrultusunda yürüyerek 25 atraplık örnekleme yapılmış, ayrıca farklı doğrultularda yürürken 8-10 adımda bir durularak alınan toplam 30 yonca sapı üzerinde bulunan yaprakbitleri ve thrips bireyleri ile galeri sineklerine ait galeriler sayılarak kaydedilmiştir. Yapılan örnekleme ve sayımlar sonucunda beş takıma ait 14 familyaya bağlı 53 fitofag tür ve altı takıma ait dokuz familyaya bağlı 20 predatör ve 10 parazitoit olmak üzere 30 doğal düşman türü elde edilmiştir. Cicadellidae, 19 tür ile en fazla tür içeren familya olmuş, bu familya içerisindeki türlerden ise Asymmetrasca decedens ve Empoasca decipiens en yoğun bulunan türler olmuştur. Lygus rugulipennis ise tüm fitofag türler içerisinde en baskın bulunan tür olarak görülmüştür. Tüm zararlı tür popülasyonlarının her defasında biçimlerden etkilenerek düşüş gösterdiği saptanmıştır. Tür sayısı bakımından zengin olan doğal düşmanlara bakıldığında ise predatör türlerin ağırlıkta olduğu ve bölgede yılda yapılan yaklaşık 7-8 biçime rağmen tarlada popülasyonlarının süreklilik arz ettiği görülmüştür.
Mostrar más [+] Menos [-]Extension of the Postharvest Life of Nectarine Using Modified Atmosphere Packaging and Potassium Permanganate Treatment
2018
Erdinc Bal
The effects of combinations of modified atmosphere packaging (MAP) with potassium permanganate (KMnO4) based ethylene scrubbers on the storage life and fruit quality of nectarine (Prunus persica cv. Bayramiç Beyazı) were investigated. Three different types of ethylene sachets (contained 3, 7 and 10 g KMnO4) were used and placed beside fruits in polypropylene baskets then lined with MAP. Fruits were stored at 0-1°C and 90% relative humidity throughout 40 day. During the cooling storage period, O2 and CO2 percentage in MAP, fruit firmness, total soluble solids, titratable acidity, ascorbic acid, total flavonoid content, total phenolic content, total antioxidant content and chilling injury (CI) were determined at 10 day interval. KMnO4 treated fruits had shown delayed ripening, reduced respiration and retained of higher firmness. As the dose of KMnO4 treatment increased, it was determined more positive effect on fruit quality. 10 g KMnO4 treatment was most effective in the retention of higher biochemical compounds and inhibition of CI symptoms. The results indicate that KMnO4 treatment, as well as MAP application, should be highly recommended for retaining the fruit quality of cold-stored ‘Bayramiç Beyazı’ nectarines and fruits treated with 10 g doses of KMnO4 could be stored for 40 days with good quality.
Mostrar más [+] Menos [-]Investigation of Oxidation Rate Relationship Between Fatty Acid Contents of St. John’s Wort (Hypericum perforatum) Oil and Nettle (Urtica dioica) Seed Oil
2018
Temel Kan Bakır
Vegetable extract oils are now widely used in health, cosmetics and food fields. The oxidation rates of these industrial oils are closely related to their chemical composition. In this study, fatty acid contents of St. John’s Wort (Hypericum perforatum) oil and Nettle seed (Urtica dioica) oil were investigated by gas chromatography (GS-MS) and the effects on lipid peroxidation time were tried to be related. The peroxidation of oil emulsions was carried out at in a ventilated incubation environment 37°C and pH 7. Pseudo first order constants of primer oxidation products (hydroperoxides) were calculated by Fe (III) SCN method and they found for St. John’s Wort oil and Nettle seed oil, (2.05±0.23)×10-1 and (6.64±0.54)×10-1 hour-1,respectively. This study was conducted by showed that the oxidation rates of St. John’s Wort and Nettle seed oils are closely related to the chemical composition as well as the saturation ratings of fatty acids.
Mostrar más [+] Menos [-]Gıda Kaynaklı Viral Gastroenteritler
2018
Duygu Alp | Hakan Kuleaşan
Virüsler, az sayıda gen taşıyan küçük bir genomdan ve bu genomu koruyup konak hücreye girişini sağlayan protein bir kılıftan oluşurlar. Diğer canlılardan farklı olarak aktif bir metabolizmaya sahip değillerdir. Çoğalmak için içine girdikleri hücrenin protein sentez ve enzim sistemlerinin kontrolünü ele geçirir, çok sayıda kopyalarını ürettikten sonra çoğunlukla hücrenin ölümüne yol açarak hücreden dışarı çıkarlar. Gıda içerisindeki sayıları değişmez ancak gıda ile iletilebilirler. Uygun işlemlerle gıdalarda etkisiz hale getirilebilirler. Hastalık etmeni bazı virüsler et, süt ve enfekte olmuş hayvanlardan gelen ürünler arasında bulunabilirler. Virüslere bağlı gastroenteritlerin sıklığının özellikle gelişmiş ülkeler başta olmak üzere giderek arttığı gözlenmektedir. Viral gastroenterit etkenlerinden Rotavirüs ve Enterik Adenovirüsler, çocuklarda akut gastroenteritlerin en sık görülen etkenleridir. Gıdalardaki virüsleri saptama yöntemleri yeteri kadar tatmin edici değildir ve gıda tedarikinin rutin olarak izlenmesinde tespiti çok zordur.
Mostrar más [+] Menos [-]Investigation of The Effectiveness of Teucrium Orientale L. Plant in Hemorrhoid Treatment
2018
Nuray Emin | Kıymet Nural | Ayşegül Güzel
Hemorrhoids are swollen vascular vein pads in the upper part of the anal canal. The deformations in these tissues cause serious disturbances, and most important one is hemorrhoid, which is a symptomatic degenerative disease. Due to various etiologic factors, weakening and disintegration of connective tissue cause this disease. Many methods have been developed in the treatment of hemorrhoids from the past to the present day, and drug therapy is used as primary care. However, surgical treatment methods are applied in advanced stages in which the disease can not be treated with medication and when there is a life-threatening risk. As alternative medicine applications, herbal remedies have been used in hemorrhoid treatment for centuries. Prepared herbal mixtures or extracts are still frequently preferred by the patients today. In this context, in the research we have carried out in Beyköy village of Havza district; Teucrium orientale L. are used by regional people in the treatment of hemorrhoids and it was detected that the awareness of this herbal treatment is limited. In this study, the efficacy of T. orientale L. plant in hemorrhoid treatment was evaluated by conducting a questionnaire survey on the patients using plant extract. Of the approximately 500 patients using plant extracts, 89 agreed to fill out the questionnaire. Due to the results of the survey, it was found that all of the patients using the extract of T. orientale L. plant are healed partly or completely, and the benefit from the extract changed according to the patient's age and the stage of the disease. There has been no study on the use of this plant in the treatment of hemorrhoids in literature, and it is the first study to be done in this respect.
Mostrar más [+] Menos [-]Dehydration Kinetics and Infusion Attributes of Microwave Dried Olive Leaves
2018
Ömer Faruk Gamlı | Özge Süfer | Tülin Eker
Olive leaves have been catching consumers’ and researchers’ great interest because of benefits on human health. In this study, the olive leaves were dried by microwave method at different powers (52, 90, 167, 290, 347 W real effective power levels) and drying kinetics of olive leaves were examined to find the best mathematical model. Page model was the most suitable model rather than the others. Diffusion coefficients were ranged between 2.65×10-10 to 6.87×10-10 m2/s and an increment in power level promoted moisture diffusivities. Dried olive leaves were used to get leave tea and different infusion temperatures were investigated to recover the total polyphenols (mg GAE/kg) and radical scavenging activities (%). Rising in infusion temperature, especially at 100°C enhanced the extraction levels of polyphenols from leave tea. Olive leaves dried at 167 W had higher phenolic contents (2282.9 mg GAE/kg) among all samples.
Mostrar más [+] Menos [-]The Antibacterial Activities of Lavandula stoechas and Crepis sancta Leaf and Flower Against Mastitis Pathogens and Enzymatic and Non-Enzymatic Antioxidant Activities of The Extracts
2018
Nuray Ergün | Gülten Ökmen | Pınar Erdal | Pınar Erdal | Zafer Cantekin | Yaşar Ergün
The antibacterial and antioxidant activities of Lavandula stoechas and Crepis sancta extracts on subclinical mastitis bacteria in cows have not been reported to the present day. The aim of this work was to investigate of the antibacterial and antioxidant potential of methanol extract from L. stoechas and C. sancta. Methanol extracts were screened for antibacterial activity against mastitis pathogens. The antibacterial activity was tested using disc diffusion and broth dilution method. In addition, the plant extracts were tested against the stable DPPH (1,1-Diphenyl-2-picrylhydrazyl) free-radical. Additionally methanol extracts were screened for enzymatic antioxidant activity. The methanol extract of C. sancta showed maximum inhibition zone against S. aureus-18 (16 mm). MIC (minimum inhibition concentration) value was determined as 3250 µg/mL for test bacteria. The highest radical scavenging capacity (62 %) was obtained from the flower extracts of L. stoechas and the leaf extracts of C. sancta. The methanol extract of C. sancta displayed a strong antioxidant activity (trolox equivalent = 1.6 mM/g). It was determined that the activities of catalase (CAT) and ascorbate peroxidase (AP) enzymes were higher at the flowers of L. stoechas compared to the leaves. The extracts of L. stoechas and C. sancta leaf and flower have antibacterial and antioxidant potential against subclinic mastitis causative agent Staphylococci species.
Mostrar más [+] Menos [-]Effect of Pulsed Ultraviolet Light on Natural Microbial Load and Antioxidant Properties of Fresh Blueberries
2018
Senem Guner | Zeynal Topalcengiz
Blueberries have been drawn more attention in recent years for their antioxidant capacities and proposed health benefits. Pulsed ultraviolet (PUV) light is known for its disinfection effects on the surface of fresh fruits. This study’s aim was to examine the effects of PUV light on naturally present microbial load, antioxidant capacity, and antioxidant enzyme activity of fresh blueberries. Fresh blueberry samples were treated for 30, 60, 90, and 120 s. Samples were stabilized at 13 cm away from the PUV lamp before treatment. Total Aerobic Plate Count (TAPC) and yeast and mold count reduction were observed the highest in samples treated for 90 s, 1.97 and 1.27 log CFU/g, respectively. Antioxidant capacity levels were significantly different for treated samples for 60 and 90 s in FRAP analysis, whereas no differences were observed in results of DPPH analysis. Superoxide dismutase (SOD) activity as antioxidant enzyme had no difference, however, glutathione peroxidase (GPx) increased by 50% with PUV treatment for 120 s. PUV light treatment of fresh blueberries for 90 s was determined as ideal exposure time among tested time intervals of this study. PUV light illumination can generally enhance antioxidant capacity and antioxidant enzyme activity of fresh blueberries and decrease the population of natural microorganisms of fresh blueberries and similar small fruits.
Mostrar más [+] Menos [-]Effect of Storage Duration and Processing Parameters on Some Cooking Properties of Ofada Rice
2018
Oludare Olumuyiwa Adekoyeni | Adedola Sulaiman Adeboye
Effects of storage duration, soaking time and parboiling temperature on cooking properties (cooking time, water uptake ratio, solid loss, cooked kernel length, and amylose) of Ofada rice was determined and optimised using response surface methodology. Storage duration, soaking time and parboiling temperature were 1, 5, and 9 months; 1, 3, and 5 days; and 80, 100, and 120°C. Data were analysed by ANOVA and regression analysis. The cooking time ranged between 14-38 min, water uptake ratio (WUR) 2.51-4.61, solid loss 1.47-4.78%, cooked kernel length 6.32-11.90 and amylose 17.34-26.28%. There exist significant differences in the cooking properties. The coefficient R2 ranged between 0.97-0.75 which is a positive indicator of the model fitness. Storage duration and parboiling temperature influenced cooking except in solid loss and cooked kernel length respectively. Effect of soaking time was found prominent in WUR and solid loss. Optimum treatment for quality cooking properties are storage of paddy for 5 months, soaking for 18h and parboiling at 80°C to yield 20 min cooking time, 4.22 water uptake ratio, 4.11% solid loss, 10.58 mm cooked kernel length and 25.08% amylose. The validated experiment yielded 21.41 min, 3.99, 2.73%, 8.20 mm and 26.39% for cooking time, water uptake ratio, solid loss, cooked kernel length and amylose respectively.
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