This study aimed to establish an adequate tetrazolium test procedure to estimate okra seed viability. Initially, seeds from four okra lots were immersed in alcohol (98%) for 60 minutes, followed by an 18-h soak in distilled water at 25 °C. Then, the seeds were longitudinally sectioned, immersed in 0.025, 0.050 and 0.075% tetrazolium solutions for 60, 90 and 120 minutes and maintained in an oven at 35 and 40 °C. Subsequently, the seeds were evaluated for viability, based on tissue color (bright red, pink or dark Carmine red). Simultaneously, a germination test was conducted, and its results were used as a reference for the tetrazolium test. The experiment was carried out in a completely randomized design, in a 3 x 3 + 1 factorial scheme (three concentrations of tetrazolium salt x three immersion periods, plus the control) in four replicates of 50 seeds for each treatment. The tetrazolium test to estimate okra seed viability should be conducted in 0.075% tetrazolium solution, for 90 minutes, at 35 °C.

RESUMO Objetivou-se estabelecer o procedimento adequado do teste de tetrazólio para estimar a viabilidade em sementes de quiabo. Inicialmente, as sementes de quatro lotes de quiabo foram imersas em álcool (98%) por 60 minutos e seguida de 18 horas de embebição em água destilada a 25 ºC. Após este período, as sementes foram seccionadas longitudinalmente, imersas em soluções de tetrazólio de 0,025; 0,050 e 0,075% por 60, 90 e 120 minutos e mantidas em estufa a 35 e 40 °C. Posteriormente, as sementes foram avaliadas quanto à viabilidade, tendo como indicação a coloração dos tecidos (vermelho brilhante rosa ou vermelho carmim forte). Paralelamente, foi instalado o teste de germinação, cujos resultados serviram como referência para o teste de tetrazólio. O experimento foi conduzido em delineamento inteiramente casualisado, em esquema fatorial 3 x 3 + 1, (três concentrações do sal de tetrazólio x três períodos de imersão e a testemunha) em quatro repetições de 50 sementes para cada tratamento. O teste de tetrazólio para estimar a viabilidade em sementes de quiabo deve ser realizado em solução de tetrazólio de 0,075%, por 90 minutos, a 35 °C. | ABSTRACT This study aimed to establish an adequate tetrazolium test procedure to estimate okra seed viability. Initially, seeds from four okra lots were immersed in alcohol (98%) for 60 minutes, followed by an 18-h soak in distilled water at 25 °C. Then, the seeds were longitudinally sectioned, immersed in 0.025, 0.050 and 0.075% tetrazolium solutions for 60, 90 and 120 minutes and maintained in an oven at 35 and 40 °C. Subsequently, the seeds were evaluated for viability, based on tissue color (bright red, pink or dark Carmine red). Simultaneously, a germination test was conducted, and its results were used as a reference for the tetrazolium test. The experiment was carried out in a completely randomized design, in a 3 x 3 + 1 factorial scheme (three concentrations of tetrazolium salt x three immersion periods, plus the control) in four replicates of 50 seeds for each treatment. The tetrazolium test to estimate okra seed viability should be conducted in 0.075% tetrazolium solution, for 90 minutes, at 35 °C.

Genome-wide selection (GWS) uses simultaneously the effect of the thousands markers covering the entire genome to predict genomic breeding values for individuals under selection. The possible benefits of GWS are the reduction of the breeding cycle, increase in gains per unit of time, and decrease of costs. However, the success of the GWS is dependent on the choice of the method to predict the effects of markers. Thus, the objective of this work was to predict genomic breeding values (GEBV) through artificial neural networks (ANN), based on the estimation of the effect of the markers, compared to the Ridge Regression-Best Linear Unbiased Predictor/Genome Wide Selection (RR-BLUP/GWS). Simulations were performed by software R to provide correlations concerning ANN and RR-BLUP/GWS. The prediction methods were evaluated using correlations between phenotypic and genotypic values and predicted GEBV. The results showed the superiority of the ANN in predicting GEBV in simulations with higher and lower marker densities, with higher levels of linkage disequilibrium and heritability.

RESUMO A seleção genômica ampla (Genome Wide Selection - GWS) utiliza simultaneamente o efeito de milhares de marcadores cobrindo todo o genoma para predizer o valor genético genômico dos indivíduos no processo de seleção. Os possíveis benefícios de seu uso são a redução do ciclo de melhoramento, propiciando maior ganho por unidade de tempo e diminuição de custos. O sucesso da GWS está atrelado a escolha do método de predição dos efeitos dos marcadores. Assim, neste trabalho, visou-se aplicar as redes neurais artificiais (Artificial Neural Networks - ANNs), com a finalidade de predizer os valores genéticos genômicos (Genomic Breeding Values - GEBVs) baseado na estimação dos efeitos dos marcadores comparados a regressão de cumeeira - melhor preditor não viesado/seleção genômica ampla (Ridge Regression - Best Linear Unbiased Predictor/Genome Wide Selection - RR-BLUP/GWS). Foram efetuadas simulações por meio do software R, fornecendo as correlações referentes às ANNs e a RR-BLUP/GWS. Os métodos de predição foram avaliados utilizando correlações entre o valor fenotípico e valor genotípico com o valor genético genômico predito. Os resultados demonstraram superioridade das ANNs na predição dos GEBVs nos cenários com maior e menor densidade de marcadores, paralelo a níveis mais altos de desequilíbrio de ligação e maior herdabilidade. | ABSTRACT Genome-wide selection (GWS) uses simultaneously the effect of the thousands markers covering the entire genome to predict genomic breeding values for individuals under selection. The possible benefits of GWS are the reduction of the breeding cycle, increase in gains per unit of time, and decrease of costs. However, the success of the GWS is dependent on the choice of the method to predict the effects of markers. Thus, the objective of this work was to predict genomic breeding values (GEBV) through artificial neural networks (ANN), based on the estimation of the effect of the markers, compared to the Ridge Regression-Best Linear Unbiased Predictor/Genome Wide Selection (RR-BLUP/GWS). Simulations were performed by software R to provide correlations concerning ANN and RR-BLUP/GWS. The prediction methods were evaluated using correlations between phenotypic and genotypic values and predicted GEBV. The results showed the superiority of the ANN in predicting GEBV in simulations with higher and lower marker densities, with higher levels of linkage disequilibrium and heritability.

Reliable information on vegetation cover and its dynamics over time is essential for managing life, resources, and supporting policies. Although many related initiatives have been undertaken in Brazil since the 1980s, knowledge about its dry forests is still limited. In this study, we address the drivers of deforestation and their threat to the Brazilian dry forest called Caatinga. We evaluated the land cover dynamics and landscape metrics in the watershed of the Pontal River, as it has become one of the most socially and environmentally important areas in Brazil. The overall process consists of a systematic sampling grid of hydrological samples, where Landsat images (1975 to 2013) combined with the FRAGSTATS package were used to evaluate landscape metrics indices for the Caatinga. Data showed that the relative area (RA) decreased from 90.25% to 60.98% over the 38-year period, while fragmentation (PD) presented an increasing bias. In addition, the spatial distribution of both indices became more heterogeneous and clustered in the north. We suggest that appropriateness of land for farming was the leading cause of deforestation; rainfall is 112% more intense in the north of the watershed than in the south. Therefore, the northern fauna and flora have been significantly altered and reduced. The relationship between deforestation and time shows an increasing threat. Further studies evaluating these causes are needed to improve our understanding of the vegetation dynamics in this region.

Reliable information on vegetation cover and its dynamics over time is essential for managing life, resources, and supporting policies. Although many related initiatives have been undertaken in Brazil since the 1980s, knowledge about its dry forests is still limited. In this study, we address the drivers of deforestation and their threat to the Brazilian dry forest called Caatinga. We evaluated the land cover dynamics and landscape metrics in the watershed of the Pontal River, as it has become one of the most socially and environmentally important areas in Brazil. The overall process consists of a systematic sampling grid of hydrological samples, where Landsat images (1975 to 2013) combined with the FRAGSTATS package were used to evaluate landscape metrics indices for the Caatinga. Data showed that the relative area (RA) decreased from 90.25% to 60.98% over the 38-year period, while fragmentation (PD) presented an increasing bias. In addition, the spatial distribution of both indices became more heterogeneous and clustered in the north. We suggest that appropriateness of land for farming was the leading cause of deforestation; rainfall is 112% more intense in the north of the watershed than in the south. Therefore, the northern fauna and flora have been significantly altered and reduced. The relationship between deforestation and time shows an increasing threat. Further studies evaluating these causes are needed to improve our understanding of the vegetation dynamics in this region.

Soil-water characteristic curve (SWCC) is an important tool for water management in irrigated agriculture. However, factors such as texture and structure of soils influence SWCC behavior. According to the literature, wetting and drying cycles alter SWCC. A similar process of re-saturation and drying occurs during SWCC obtainment under laboratory conditions. Based on the hypothesis that re-saturation process alters SWCC due to clay loss in the sample, this study aimed to obtain the SWCC, S index, and pore size distribution from samples submitted to re-saturation cycles, as well as from not re-saturated samples but under higher matric potentials (-2, -4, -6, -8, and -10 kPa). For this, disturbed and undisturbed soil samples, collected from the A (sandy texture) and Btg (sandy clay loam texture) horizons of a Argissolo Acizentado, were used. After obtaining SWCC, each air-dried soil sample was submitted to particle size and clay dispersed in water analyses to verify whether the soil lost clay. The experimental design was a completely randomized design with two methods of SWCC constructing (with and without re-saturation) and eight replications. The re-saturation process generates a loss of clay in the sample, not causing significant changes in SWCC considering the assessed textural soil classes. In addition, sandy soil samples are more sensitive to changes in pore size distribution when submitted to re-saturation.

RESUMO A curva característica de água no solo (CCAS) constitui importante ferramenta no manejo da água na agricultura irrigada. Entretanto, vale ressaltar que fatores como textura e estrutura do solo influenciam seu comportamento.Há informações na literatura que ciclos de umedecimento e secagem alteram a CCAS. Saliente-se que processo similar, de ressaturação e secagem, ocorre durante a obtenção da CCAS em laboratório. Tendo como hipótese que o processo de ressaturação, por provocar perda de argila na amostra, altera a CCAS, objetivou-se obter a CCAS, o índice S e a distribuição dos poros por tamanho a partir de amostras submetidas a ciclos de ressaturação em oposição àquelas não sujeitas a este processo em maiores potenciais mátricos (-2, -4, -6, -8 e -10 kPa). Para tanto, utilizaram-se amostras com estrutura deformada e indeformada, coletadas do horizonte A (textura arenosa) e Btg (textura franco argilo-arenosa) de um Argissolo Acinzentado. Após a obtenção da CCAS, cada amostra foi levada à condição de terra fina seca ao ar e submetida à análise granulométrica e de argila dispersa em água para verificar se houve perda de argila. O delineamento estatístico foi o inteiramente casualizado, com dois métodos de construção da CCAS - com e sem ressaturação - e oito repetições. Concluiu-se que o processo de ressaturação gera perda de argila na amostra, mas não provoca alterações significativas na CCAS nas classes texturais avaliadas; e que as amostras de solo de textura arenosa são mais sensíveis a alterações na distribuição de poros por tamanho quando submetidas à ressaturação. | ABSTRACT Soil-water characteristic curve (SWCC) is an important tool for water management in irrigated agriculture. However, factors such as texture and structure of soils influence SWCC behavior. According to the literature, wetting and drying cycles alter SWCC. A similar process of re-saturation and drying occurs during SWCC obtainment under laboratory conditions. Based on the hypothesis that re-saturation process alters SWCC due to clay loss in the sample, this study aimed to obtain the SWCC, S index, and pore size distribution from samples submitted to re-saturation cycles, as well as from not re-saturated samples but under higher matric potentials (-2, -4, -6, -8, and -10 kPa). For this, disturbed and undisturbed soil samples, collected from the A (sandy texture) and Btg (sandy clay loam texture) horizons of a Argissolo Acizentado, were used. After obtaining SWCC, each air-dried soil sample was submitted to particle size and clay dispersed in water analyses to verify whether the soil lost clay. The experimental design was a completely randomized design with two methods of SWCC constructing (with and without re-saturation) and eight replications. The re-saturation process generates a loss of clay in the sample, not causing significant changes in SWCC considering the assessed textural soil classes. In addition, sandy soil samples are more sensitive to changes in pore size distribution when submitted to re-saturation.

Sesame is a plant with high morphological and physiological complexity, with great variability in growth habit. Quantitative growth analysis is an accessible and accurate tool for evaluating plant development and the contribution of different physiological processes to plant performance. In view of this, the purpose of this study was to evaluate the growth of sesame cultivars in two cropping seasons in the conditions of Mossoró-RN. Two experiments were conducted in Horta Didactics of UFERSA. The experimental delineation in each time was a randomized complete block design with four replications. The treatments were arranged in split plots where each experimental plot contained different sesame cultivars, CNPA G2, CNPA G3 and CNPA G4, and the subplots represented seven collection times, 21, 35, 49, 63, 77, 91 and 105 days after sowing (DAS). The growth of the sesame cultivars was slow at the beginning of the crop cycle, intensifying at the beginning of flowering (after 35 DAS). Among the physiological indexes studied, CNPA G4 cultivar was more efficient in relation to growth and varied depending on the cropping season.

RESUMO O gergelim é uma cultura de elevada complexidade morfofisiológica, com grande variabilidade no hábito de crescimento. A análise quantitativa do crescimento é uma ferramenta acessível e precisa para avaliar o desenvolvimento vegetal e a contribuição de diferentes processos fisiológicos sobre o seu desempenho. Nesse sentido, objetivou-se avaliar o crescimento de cultivares de gergelim em duas épocas de cultivo nas condições de Mossoró-RN. Foram instalados dois experimentos na Horta Didática da UFERSA. O delineamento experimental em cada época foi em blocos casualizados completos, com quatro repetições. Os tratamentos foram dispostos em parcelas subdivididas, onde as parcelas foram representadas pelas cultivares de gergelim: CNPA G2, CNPA G3 e CNPA G4 e as subparcelas consistiram das épocas de coletas (21; 35; 49; 63; 77; 91 e 105 DAS). O crescimento das cultivares de gergelim é lento no início do ciclo vegetativo da cultura, se intensificando no início do florescimento (após os 35 DAS). Entre os índices fisiológicos estudados, a cultivar CNPA G4 apresenta ser mais eficiente em relação ao crescimento, podendo ser variado dependendo da época de cultivo. | ABSTRACT Sesame is a plant with high morphological and physiological complexity, with great variability in growth habit. Quantitative growth analysis is an accessible and accurate tool for evaluating plant development and the contribution of different physiological processes to plant performance. In view of this, the purpose of this study was to evaluate the growth of sesame cultivars in two cropping seasons in the conditions of Mossoró-RN. Two experiments were conducted in Horta Didactics of UFERSA. The experimental delineation in each time was a randomized complete block design with four replications. The treatments were arranged in split plots where each experimental plot contained different sesame cultivars, CNPA G2, CNPA G3 and CNPA G4, and the subplots represented seven collection times, 21, 35, 49, 63, 77, 91 and 105 days after sowing (DAS). The growth of the sesame cultivars was slow at the beginning of the crop cycle, intensifying at the beginning of flowering (after 35 DAS). Among the physiological indexes studied, CNPA G4 cultivar was more efficient in relation to growth and varied depending on the cropping season.

The low use of technologies by farmers and the occurrence of biotic and abiotic stresses are limiting factors for cowpea production in the Brazilian Northeast region. The tolerance of genotypes to drought is an alternative to decrease the negative effects of stresses on cowpea production. Thus, the objective of this work was to identify parents and combinations of cowpea genotypes with high probability of generating segregating populations with tolerance to drought. Six cowpea genotypes were crossed in a complete diallel cross design, totaling 30 F2 populations, which were evaluated together with their parents in an experiment under water deficit at the experimental field of the Embrapa Mid-North, Teresina, State of Piauí, Brazil. A triple lattice incomplete block experiment design was used, with three replications, with experimental plot consisting of six 2-m plant rows. Fifteen plants per plot were sampled to evaluate their agronomic characteristics, whose results were subjected to analysis of variance and means were used to estimate the general and specific combining abilities. The genotypes showed significant differences in all characteristics evaluated, denoting the genetic variability of the population. The additive effects were more important than the non-additive effects, and maternal inheritance was detected. The genotypes BRS Xiquexique, Pingo-de-Ouro-1-2 and MNC99-510F-16-1 were the most promising for use in recurrent selection programs for tolerance to water deficit. The hybrid combinations (1) BRS Paraguaçu X (4) CNCx-698-128G, (2) Pingo-de-Ouro-1-2 X (3) BRS Xiquexique, (3) BRS Xiquexique X (5) Santo-Inácio, (4) CNCx-698-128G X (6) MNC99-510F-16-1 and (5) Santo-Inácio X (4) CNCx-698-128G showed potential for generating superior lineages regarding bean production and tolerance to water deficit.

ABSTRACT The low use of technologies by farmers and the occurrence of biotic and abiotic stresses are limiting factors for cowpea production in the Brazilian Northeast region. The tolerance of genotypes to drought is an alternative to decrease the negative effects of stresses on cowpea production. Thus, the objective of this work was to identify parents and combinations of cowpea genotypes with high probability of generating segregating populations with tolerance to drought. Six cowpea genotypes were crossed in a complete diallel cross design, totaling 30 F2 populations, which were evaluated together with their parents in an experiment under water deficit at the experimental field of the Embrapa Mid-North, Teresina, State of Piauí, Brazil. A triple lattice incomplete block experiment design was used, with three replications, with experimental plot consisting of six 2-m plant rows. Fifteen plants per plot were sampled to evaluate their agronomic characteristics, whose results were subjected to analysis of variance and means were used to estimate the general and specific combining abilities. The genotypes showed significant differences in all characteristics evaluated, denoting the genetic variability of the population. The additive effects were more important than the non-additive effects, and maternal inheritance was detected. The genotypes BRS Xiquexique, Pingo-de-Ouro-1-2 and MNC99-510F-16-1 were the most promising for use in recurrent selection programs for tolerance to water deficit. The hybrid combinations (1) BRS Paraguaçu X (4) CNCx-698-128G, (2) Pingo-de-Ouro-1-2 X (3) BRS Xiquexique, (3) BRS Xiquexique X (5) Santo-Inácio, (4) CNCx-698-128G X (6) MNC99-510F-16-1 and (5) Santo-Inácio X (4) CNCx-698-128G showed potential for generating superior lineages regarding bean production and tolerance to water deficit. | RESUMO A baixa adoção de tecnologias pelos agricultores e a ocorrência de estresses bióticos e abióticos são fatores que limitam a produção do feijão-caupi na região Nordeste do Brasil. A tolerância de genótipos à seca é uma das formas para diminuir os efeitos negativos dos estresses sobre a produtividade. O objetivo deste trabalho foi identificar genitores e combinações promissoras com maior probabilidade de gerarem populações segregantes de feijão-caupi tolerantes à seca. Foi realizado um dialelo completo envolvendo seis genótipos de feijão-caupi, totalizando 30 populações F2, que foram avaliadas, juntamente com seus genitores, em um ensaio sob déficit hídrico, no campo experimental da Embrapa Meio-Norte, em Teresina-PI. Utilizou-se o delineamento experimental látice triplo, com parcela de 6 linhas de 2 m, sendo amostradas 15 plantas por parcela. Foram avaliados caracteres agronômicos e submetidos à análise de variância e, utilizando-se das médias, obtiveram-se as estimativas de capacidade geral e específica de combinação. Observou-se diferenças significativas para todos os caracteres, evidenciando a existência de variabilidade genética na população. Os efeitos aditivos foram mais importantes que os efeitos não aditivos, sendo também constatada a presença de herança materna. Os genótipos BRS Xiquexique, Pingo de Ouro-1-2 e MNC99- 510F-16-1 foram os mais promissores para utilização em programa de seleção recorrente visando tolerância ao déficit hídrico. As combinações híbridas (1) BRS Paraguaçu X (4) CNCx 698-128G, (2) Pingo de Ouro-1-2 X (3) BRS Xiquexique, (3) BRS Xiquexique X (5) Santo Inácio, (4) CNCx 698-128G X (6) MNC99-510F-16-1 e (5) Santo Inácio X (4) CNCx 698-128G, possuem potencial simultâneos para extração de linhagens superiores quanto à produtividade de grãos e tolerantes ao déficit hídrico.

The northeastern semiarid region faces some of the droughts of the last centuries, resulting in serious reductions of agriculture yields. The water scarcity for agricultural requires rational use practices and reuse of water, such as use of domestic effluent sewage. Alternatively, the use of treated domestic sewage effluent utilizing for irrigation of forage species, such as the forage cactus, can be highlighted. In order to evaluate the effects of fertirrigation with treated sewage effluent on mineral nutrient composition of forage cactus (Opuntia Tuna L. Mill, Mexican elephant ear), an experiment was carried out utilizing a split plot 3 x (4 + 1) with a randomized complete block design randomized block and four replications, being three planting density (66,667.00, 52,282.00 and 33,333.00 plants ha-1) and two water sources associated with irrigation frequency of plates fixed on 3.5 mm per irrigation (Frequencies of 2.3 days with water tap and 7, 14 and 21 days with Sewage Effluent) and cactus cultivated in Rainfed agriculturerainfed (control). The analyze of the nutritional contents of cladodes indicated that there was a significant effect of the intervals from irrigation events only for Ca and Mg concentrations. The highest levels of Ca and Mg were found with the irrigation frequency of 2.3 using tap water. The macronutrients concentration in greater quantity by the forage cactus were N e, K followed by, Ca, Mg and P. The nutritional balance indicated excess of K and P and deficiency of N, Ca and Mg, in this order.

RESUMO O semiárido nordestino enfrenta umas das mais severas secas dos últimos séculos, resultando em reduções severas na produção agrícola. A escassez hídrica na agricultura exige práticas de uso racional e reaproveitamento da água como, por exemplo, a utilização de efluentes de esgoto doméstico . Alternativamente pode-se destacar o uso de esgoto doméstico tratado para irrigação de espécies forrageiras, como a palma. Objetivando avaliar os efeitos da fertirrigação com efluente de esgoto doméstico tratado sob a composição mineral de nutriente em palma forrageira (Opuntia Tuna L. Mill, Orelha-de-elefante Mexicana), foi conduzido um experimento em blocos casualizados em esquema de parcelas subdivididas 3 x (4 + 1), com 4 repetições, sendo três densidades de plantio (66.667,00; 52.282,00 e 33.333,00 plantas ha-1) e duas fontes hídrica associadas a frequência de irrigação com lâmina fixa de 3,5 mm por rega (Frequências de 2,3 dias com água de abastecimento e de 7, 14 e 21 dias com Efluente de Esgoto) e, palma cultivada em sequeiro (testemunha). As análises dos teores nutricionais dos cladódios indicam que houve efeito significativo dos intervalos entre eventos de irrigação apenas para as concentrações de Ca e Mg. Os maiores teores de Ca e Mg foram encontrados com a frequência de irrigação de 2,3 utilizando água de abastecimento. Os macronutrientes em maior quantidade pela palma forrageira foram N e K seguido de Ca, Mg e P. O balanço nutricional indicou excesso de K e P e déficit de N, Ca e Mg, nesta ordem. | ABSTRACT The northeastern semiarid region faces some of the droughts of the last centuries, resulting in serious reductions of agriculture yields. The water scarcity for agricultural requires rational use practices and reuse of water, such as use of domestic effluent sewage. Alternatively, the use of treated domestic sewage effluent utilizing for irrigation of forage species, such as the forage cactus, can be highlighted. In order to evaluate the effects of fertirrigation with treated sewage effluent on mineral nutrient composition of forage cactus (Opuntia Tuna L. Mill, Mexican elephant ear), an experiment was carried out utilizing a split plot 3 x (4 + 1) with a randomized complete block design randomized block and four replications, being three planting density (66,667.00, 52,282.00 and 33,333.00 plants ha-1) and two water sources associated with irrigation frequency of plates fixed on 3.5 mm per irrigation (Frequencies of 2.3 days with water tap and 7, 14 and 21 days with Sewage Effluent) and cactus cultivated in Rainfed agriculturerainfed (control). The analyze of the nutritional contents of cladodes indicated that there was a significant effect of the intervals from irrigation events only for Ca and Mg concentrations. The highest levels of Ca and Mg were found with the irrigation frequency of 2.3 using tap water. The macronutrients concentration in greater quantity by the forage cactus were N e, K followed by, Ca, Mg and P. The nutritional balance indicated excess of K and P and deficiency of N, Ca and Mg, in this order.

Maize is an important cereal and it is widely consumed in the world, both as food for humans and animals. Nitrogen (N) is a nutrient required in large quantities by maize, but unfortunately, soils are limited in meeting this need. Nodulating legumes can serve as a source of Nitrogen, because they are symbiotically associated with bacteria capable of fixing atmospheric N. Another important source of this nutrient is cattle manure, which is widely used in agriculture. The objective of the present study was to evaluate the effect of using legumes and cattle manure on the production as well as the microbial and biochemical quality of the soil used for maize cultivation. The experiment was conducted using a randomized complete block design, in a sub-subdivided plot scheme (2x4) +2, two leguminous plants (pigeon pea and macassar bean), four doses of cattle manure (0, 20, 40 and 60 t ha-1) and two controls; one with mineral fertilization and the other cultivated without the use of fertilizers. Treatment using a manure dose of 60t ha-1 and pigeon pea legume, recorded higher dry mass production per plant. The increase in manure doses was directly proportional to the length and weight of the cobs for the two legumes. The enzymatic activities were affected by the different doses and legumes, with greater results being recorded at the 60 t ha-1 dose with the macassar legume. The use of legumes with manure improved maize production as well as the microbiological and biochemical quality of soils.

ABSTRACT Maize is an important cereal and it is widely consumed in the world, both as food for humans and animals. Nitrogen (N) is a nutrient required in large quantities by maize, but unfortunately, soils are limited in meeting this need. Nodulating legumes can serve as a source of Nitrogen, because they are symbiotically associated with bacteria capable of fixing atmospheric N. Another important source of this nutrient is cattle manure, which is widely used in agriculture. The objective of the present study was to evaluate the effect of using legumes and cattle manure on the production as well as the microbial and biochemical quality of the soil used for maize cultivation. The experiment was conducted using a randomized complete block design, in a sub-subdivided plot scheme [(2x4) +2], two leguminous plants (pigeon pea and macassar bean), four doses of cattle manure (0, 20, 40 and 60 t ha-1) and two controls; one with mineral fertilization and the other cultivated without the use of fertilizers. Treatment using a manure dose of 60t ha-1 and pigeon pea legume, recorded higher dry mass production per plant. The increase in manure doses was directly proportional to the length and weight of the cobs for the two legumes. The enzymatic activities were affected by the different doses and legumes, with greater results being recorded at the 60 t ha-1 dose with the macassar legume. The use of legumes with manure improved maize production as well as the microbiological and biochemical quality of soils. | RESUMO O milho é um importante cereal muito utilizado no mundo, tanto como alimentação para humanos como para animais. O nitrogênio (N) é um nutriente requerido em grande quantidade pelo milho, e os solos são limitantes em atender essa necessidade. As leguminosas nodulantes podem ser utilizadas como fonte de Nitrogênio, por formarem associações simbióticas com bactérias capazes de fixar N atmosférico, outra importante fonte desse nutriente é o esterco bovino, utilizado largamente na agricultura. O objetivo do presente estudo foi avaliar o efeito do uso de leguminosas e esterco bovino na produção e qualidade microbiana e bioquímica do solo cultivado com milho. O experimento foi conduzido em delineamento de blocos ao acaso, em esquema de parcela sub-subdividida [(2x4) +2], sendo duas leguminosas (feijão guandu anão e feijão macassar), quatro doses de esterco bovino (0, 20, 40 e 60 t ha-1), mais dois tratamentos controle um com adubação mineral e outro sem o emprego de adubos. O tratamento utilizando a dose 60 t ha-1 de esterco com a leguminosa Guandu anão apresentou maior produção de massa seca por planta. O aumento das doses de esterco foi diretamente proporcional ao comprimento e peso das espigas para as duas leguminosas. As atividades enzimáticas responderam às diferentes doses e leguminosas, com maiores resultados na dose de 60 t ha-1 com a leguminosa macassar. O uso de leguminosa com esterco melhorou a produção de milho e a qualidade microbiológica e bioquímica dos solos.

Grape orchards are highly affected by oviposition of fruit flies on grape berries, which compromises the productivity and quality of the grapes. The goal of this study was to evaluate the susceptibility of American, European, and hybrid grape genotypes to Anastrepha fraterculus and Ceratitis capitata (Diptera: Tephritidae) under laboratory conditions (25 ± 2ºC, 60 ± 10% RH, and 14-hour photophase). The assays were conducted by evaluating oviposition preference through choice and no-choice (antibiosis) tests. The choice test was set up using circular arenas (diameter: 300 mm) with 10 grapes per genotype. The no-choice test was set up using 150 grapes per genotype which were placed inside plastic boxes (417 x 297 x 289 mm). After exposure of the grapes to one or two couples of fruit flies per genotype in choice and no-choice tests, respectively, the grapes were transferred to transparent plastic containers (750 mL). The number of eggs per grape and its viability were evaluated. The no-choice test also evaluated the biological cycle of the fruit flies. The assays were conducted in a completely randomized design with 15 replicates. The most preferred grapes for oviposition by A. fraterculus were Cabernet Sauvignon, Niagara Rosada, and BRS Cora, while C. capitata mainly preferred Isabel Precoce grapes. We observed the complete development of fruit flies in the Moscato Embrapa grapes, but this only occurred with C. capitata. All genotypes evaluated were considered susceptible to A. fraterculus and C. capitata. However, the fruit flies expressed differences in preference for oviposition and host quality.

ABSTRACT Grape orchards are highly affected by oviposition of fruit flies on grape berries, which compromises the productivity and quality of the grapes. The goal of this study was to evaluate the susceptibility of American, European, and hybrid grape genotypes to Anastrepha fraterculus and Ceratitis capitata (Diptera: Tephritidae) under laboratory conditions (25 ± 2ºC, 60 ± 10% RH, and 14-hour photophase). The assays were conducted by evaluating oviposition preference through choice and no-choice (antibiosis) tests. The choice test was set up using circular arenas (diameter: 300 mm) with 10 grapes per genotype. The no-choice test was set up using 150 grapes per genotype which were placed inside plastic boxes (417 x 297 x 289 mm). After exposure of the grapes to one or two couples of fruit flies per genotype in choice and no-choice tests, respectively, the grapes were transferred to transparent plastic containers (750 mL). The number of eggs per grape and its viability were evaluated. The no-choice test also evaluated the biological cycle of the fruit flies. The assays were conducted in a completely randomized design with 15 replicates. The most preferred grapes for oviposition by A. fraterculus were Cabernet Sauvignon, Niagara Rosada, and BRS Cora, while C. capitata mainly preferred Isabel Precoce grapes. We observed the complete development of fruit flies in the Moscato Embrapa grapes, but this only occurred with C. capitata. All genotypes evaluated were considered susceptible to A. fraterculus and C. capitata. However, the fruit flies expressed differences in preference for oviposition and host quality. | RESUMO No cultivo da videira a oviposição das moscas-das-frutas compromete a produtividade e a qualidade de bagas de uva. O objetivo desse trabalho foi avaliar a suscetibilidade de genótipos de uvas americanas, europeias e híbridas à Anastrepha fraterculus e Ceratitis capitata (Diptera: Tephritidae) em laboratório (25 ± 2ºC, 60±10% UR e fotofase de 14 horas) por meio de ensaios de preferência de oviposição com e sem chance de escolha (antibiose). No ensaio com chance de escolha foram utilizadas arenas circulares (300 mm de diâmetro) com 10 bagas por genótipo de uva. No teste sem chance de escolha foram utilizadas 150 bagas por genótipo de uva acondicionadas em caixa plástica (417 x 297 x 289 mm). Após a exposição a um ou a dois casais de moscas-das-frutas, por genótipo de uva nos testes com e sem chance de escolha, respectivamente, as bagas foram transferidas para potes plásticos transparentes (750 mL). Nos ensaios foram avaliados o número de ovos por baga e sua viabilidade. No ensaio sem chance de escolha também foi avaliado o ciclo biológico das moscas-das-frutas (teste de antibiose). Os experimentos foram conduzidos em delineamento inteiramente casualizado com 15 repetições. As bagas de uvas preferidas para oviposição por A. fraterculus foram Cabernet Sauvignon, Niágara Rosada e BRS Cora. Para C. capitata foi a Isabel Precoce. No genótipo Moscato Embrapa foi observado o desenvolvimento de ovo-adulto apenas de C. capitata. Assim, todos os genótipos foram considerados suscetíveis à A. fraterculus e C. capitata. No entanto, expressam diferenças na preferência para oviposição e na qualidade hospedeira para A. fraterculus e C. capitata.

The objective of this study was to evaluate the productive and economic performance of a forage cactus and sorghum intercropping system in relation to a forage cactus single cropping system under different irrigation blades with saline water. The study was conducted in Serra Talhada, State of Pernambuco, in a randomized block design, consisting of a factorial arrangement with split-plot parcels (5×2) and four replications. The five plots were the irrigation depths based on the reference evapotranspiration (ETo) (0, 8.75, 17.5, 26.25, and 35% ETo) and the two cropping systems were single forage cactus cropping and cactus intercropped with sorghum. The experiment was conducted for one year, with one forage cactus cycle and two cycles of sorghum. Yields were obtained at the end of the cycles. Economic profitability was analyzed by means of net revenue (NR) and the benefit/cost ratio (B/C), with cactus forage sold as forage or as "seed" and sorghum as forage. Net revenue and B/C simulations were carried out over an eight-year useful life span of the irrigation system. Increasing irrigation depth increased the dry matter yield of the intercropping system because of higher sorghum yield. Based on the NR and B/C ratio values, forage cactus and sorghum produced as forage produced higher economic outcomes in the intercropping system and showed a higher profitability from the 2nd year of implementation of the irrigation system. Forage cactus and sorghum intercropping irrigated with saline water is therefore recommended for forage production in the Brazilian semi-arid region.

RESUMO Objetivou-se avaliar o desempenho produtivo-econômico da consorciação palma forrageira e sorgo em relação ao sistema de plantio exclusivo da palma forrageira sob diferentes lâminas de irrigação com água salina. A pesquisa foi conduzida em Serra Talhada, PE, com delineamento em blocos ao acaso, arranjo fatorial com parcelas subdivididas (5×2), quatro repetições, sendo considerada como parcelas as lâminas de irrigação complementar com base na evapotranspiração de referência (0; 8,75; 17,5; 26,25 e 35% ETo), e as subparcelas, os sistemas de plantio: palma exclusiva e consórcio palma-sorgo. O experimento foi conduzido por um ano, sendo um ciclo de palma e dois ciclos do sorgo. Ao final foram obtidas as produtividades de matéria fresca e seca. A rentabilidade econômica foi analisada por meio da receita líquida (RL) e da relação benefício/custo (B/C) da palma destinada à venda tanto para forragem como “semente” para o plantio, e do sorgo para produção de forragem. Simulações de RL e B/C foram feitas ao longo da vida útil de oito anos do sistema de irrigação. As lâminas de água aumentaram a produtividade de matéria seca do consórcio, devido ao benefício gerado ao sorgo. Com base na RL e B/C, verificou-se que a palma e o sorgo destinados à venda como forragem mostraram mais vantagem quando consorciadas e maior rentabilidade a partir do 2º ano de implantação do sistema de irrigação. Conclui-se que, o consórcio palma forrageira e sorgo irrigado com água salina deve ser recomendado para a produção de forragem no Semiárido brasileiro. | ABSTRACT The objective of this study was to evaluate the productive and economic performance of a forage cactus and sorghum intercropping system in relation to a forage cactus single cropping system under different irrigation blades with saline water. The study was conducted in Serra Talhada, State of Pernambuco, in a randomized block design, consisting of a factorial arrangement with split-plot parcels (5×2) and four replications. The five plots were the irrigation depths based on the reference evapotranspiration (ETo) (0, 8.75, 17.5, 26.25, and 35% ETo) and the two cropping systems were single forage cactus cropping and cactus intercropped with sorghum. The experiment was conducted for one year, with one forage cactus cycle and two cycles of sorghum. Yields were obtained at the end of the cycles. Economic profitability was analyzed by means of net revenue (NR) and the benefit/cost ratio (B/C), with cactus forage sold as forage or as "seed" and sorghum as forage. Net revenue and B/C simulations were carried out over an eight-year useful life span of the irrigation system. Increasing irrigation depth increased the dry matter yield of the intercropping system because of higher sorghum yield. Based on the NR and B/C ratio values, forage cactus and sorghum produced as forage produced higher economic outcomes in the intercropping system and showed a higher profitability from the 2nd year of implementation of the irrigation system. Forage cactus and sorghum intercropping irrigated with saline water is therefore recommended for forage production in the Brazilian semi-arid region.