This study aimed to establish an adequate tetrazolium test procedure to estimate okra seed viability. Initially, seeds from four okra lots were immersed in alcohol (98%) for 60 minutes, followed by an 18-h soak in distilled water at 25 °C. Then, the seeds were longitudinally sectioned, immersed in 0.025, 0.050 and 0.075% tetrazolium solutions for 60, 90 and 120 minutes and maintained in an oven at 35 and 40 °C. Subsequently, the seeds were evaluated for viability, based on tissue color (bright red, pink or dark Carmine red). Simultaneously, a germination test was conducted, and its results were used as a reference for the tetrazolium test. The experiment was carried out in a completely randomized design, in a 3 x 3 + 1 factorial scheme (three concentrations of tetrazolium salt x three immersion periods, plus the control) in four replicates of 50 seeds for each treatment. The tetrazolium test to estimate okra seed viability should be conducted in 0.075% tetrazolium solution, for 90 minutes, at 35 °C.

Low grain yield of cowpea is influenced by several production factors, especially inadequate soil fertilization, in particular, phosphorus application. Tropical soils have low phosphorus and zinc levels due to either the source material or the absence of fertilization, which restricts cowpea grain yield; besides of that, zinc deficiency is accentuated with phosphor application. This study aimed to evaluate the effect of fertilization with phosphorus and zinc on cowpea yield. Two experiments were conducted, one with ‘BRS Guariba’ cultivar and another with ‘BRS Aracê’ cultivar. Both of them were performed on a medium-texture dystrophic Yellow Latosol (Oxisol), in the city of Magalhães de Almeida, Maranhão state (Brazil), in 2015. The experimental design was a randomized complete block in a 4 x 4 factorial scheme, using four doses of phosphorus (0, 40, 80, and 120 kg P2O5 ha-1), and four of zinc (0, 2, 4, and 6 kg of Zn ha-1). There was an interaction between these minerals (p<0.01). Maximum grain yields of 1,376 kg ha-1 and 2,165 kg ha-1 were obtained at doses of 118 kg P2O5 ha-1 and 0.9 kg zinc ha-1 for ‘BRS Guariba’, and 120 kg P2O5 ha-1 and 3.1 kg zinc ha-1 for ‘BRS Aracê’, respectively. The production component of greatest influence on grain yield was the number of pods per plant, with average values of 6 and 10 for ‘BRS Guariba’ and ‘BRS Aracê’ cultivars, respectively. The mean correlation coefficient was 0.82 (p < 0.01).

Water deficit is a limiting factor for the soybean yield; it triggers different physiological and anatomical adaptations that have deleterious effects on the plants and can affect the selectivity of herbicides, causing production losses. In this context, the objective of this work was to evaluate the action of the chlorimuron-ethyl herbicide when applied at different stages of soybean plants, using conventional and transgenic cultivars, and different soil water potentials. A rate of 20 g ha-1 of the chlorimuron-ethyl herbicide was applied to two soybean cultivars (MG/BR46-Conquista - conventional, and BRS-Valiosa-RR - transgenic) at two phenological stages (V2 - first fully expanded trifoliate leaves, and V4 - third fully expanded trifoliate leaves), using three soil water potentials (-0.03 MPa, -0.07 MPa, and -0.5 MPa). Phytotoxicity, and plant height were evaluated at 3, 7, 14, and 21 days after the herbicide application. The shoot dry weight, root dry weight, and root system nodulation were evaluated. The soybean plants had lower phytotoxicity when subjected to application of chlorimuron-ethyl under water deficit conditions. The use of chlorimuron-ethyl reduced the growth and biomass of soybean plants and affected the plants' root system nodulation. The transgenic cultivar (BRS-Valiosa-RR) presented better performance when subjected to a moderate water deficit (-0.07 MPa), which contributes to biological nitrogen fixation.

Irrigation with saline water is a worldwide necessity an excess of salts in water or in soil causes growth inhibition, and negatively affects the productivity of many crops. Application of nitrogen fertilizers may be a way of mitigating the effects of salts on plants. The aim of this study was to evaluate the accumulation of biomass and the chlorophyll pigment content in jackfruit seedlings irrigated with water of increasing salinity level in soil with nitrogen sources. The treatments were distributed in randomized blocks with four replicates and three plants per plot, arranged in a 5 × 3 factorial scheme, related to electrical conductivity levels of the irrigation water of 0.3, 1.0, 2.0, 3.0 and 4.0 dS m-1, in soil without nitrogen, with ammonium sulfate and with urea, in a split application 60 and 75 days after sowing. An increase in the salinity of the water increased the salinity levels of soil, which was intensified by a dose of 150 mg of N, mainly when applied in the form of ammonium sulfate, inhibiting dry matter production and chlorophyll content in jackfruit seedlings. The greatest reductions in chlorophyll a and b content occurred in jackfruit seedlings irrigated with water of 4.0 dS m-1 conductivity in the soil without nitrogen fertilization. Urea is the most suitable nitrogen source for the production of seedlings under conditions of high salinity.

Vetiver grass is a member of the grass family Poaceae. Its fast development is probably due to the interaction with native microbiota, whose influence has not been studied yet. The objective of this work was to evaluate the colonization and development of the vetiver grass (Chrysopogon zizanioides (L.) Roberty) inoculated with arbuscular mycorrhizal fungi and dark septate endophytic fungi. The experimental design was a completely randomized design with six treatments (control, without mycorrhizal fungi, native inoculants, UFLA05 - Gigaspora albida, UFLA351 - Rhizoglomus clarum, UFLA372 - Claroideoglomus etunicatum, and UFLA401 - Acaulospora morrowiae), with three replicates each. Vetiver grass tillers as well as the native microbial inoculum were obtained from the Lower São Francisco river experimental area, located in Sergipe state, Northeastern Brazil. There was a negative interaction between all tested UFLAs mycorrhizal isolates and the native microbiota (mycorrhizal and endophytic fungi) in the treatments, especially when taking into consideration plant height and volume of roots. The effects of inoculation with UFLA isolates may have been influenced by the presence of the native mycorrhizal fungi and the dark septate endophytic fungi. Vetiver grass was responsive to the native inoculant. The mycorrhizal colonization of the vetiver grass was vesicular, but the formation of the arbuscules can be influenced by the interaction between the fungus, plant, and the environment.

This study aimed to determine the doses of maximum agronomic and economic efficiency as a function of different amounts of roostertree Calotropis procera (Ait.) R.Br. biomass added to the soil, that results in the maximum yield of green grains of cowpea in the semi-arid of Rio Grande do Norte state, Brazil. The experiment was conducted at the “Rafael Fernandes” Experimental Farm of the Universidade Federal Rural do Semi-Árido (UFERSA), Alagoinha, RN, from August to November 2013. In the experiment was used a randomized completely block design with 5 replicates. The treatments consisted of 20, 35, 50, and 65 t ha-1 (on a dry matter basis) of roostertree biomass added to the soil. The evaluated characteristics were: number of green pods per m2, productivity and dry mass of green pods, number of green grains per pod, weight of 100 green grains, and dry mass of green grains. The following economic indicators were determined: gross and net incomes, production operational costs, rate of return, and profit margin. The maximum agronomic efficiency of the yield of cowpea green grain was reached at the yield of 3.05 t ha-1, using 61.0 t ha-1 of roostertree biomass in the soil. The maximum economic efficiency yielded a net income of R$ 8,701.42, at the production of 3.02 t ha-1 green grains with 53.57 t ha-1 of roostertree biomass added to the soil. The use of roostertree as a green manure presents technical-economic feasibility in cowpea cultivation for green grains in the semi-arid conditions of Rio Grande do Norte.

Reliable information on vegetation cover and its dynamics over time is essential for managing life, resources, and supporting policies. Although many related initiatives have been undertaken in Brazil since the 1980s, knowledge about its dry forests is still limited. In this study, we address the drivers of deforestation and their threat to the Brazilian dry forest called Caatinga. We evaluated the land cover dynamics and landscape metrics in the watershed of the Pontal River, as it has become one of the most socially and environmentally important areas in Brazil. The overall process consists of a systematic sampling grid of hydrological samples, where Landsat images (1975 to 2013) combined with the FRAGSTATS package were used to evaluate landscape metrics indices for the Caatinga. Data showed that the relative area (RA) decreased from 90.25% to 60.98% over the 38-year period, while fragmentation (PD) presented an increasing bias. In addition, the spatial distribution of both indices became more heterogeneous and clustered in the north. We suggest that appropriateness of land for farming was the leading cause of deforestation; rainfall is 112% more intense in the north of the watershed than in the south. Therefore, the northern fauna and flora have been significantly altered and reduced. The relationship between deforestation and time shows an increasing threat. Further studies evaluating these causes are needed to improve our understanding of the vegetation dynamics in this region.

The low use of technologies by farmers and the occurrence of biotic and abiotic stresses are limiting factors for cowpea production in the Brazilian Northeast region. The tolerance of genotypes to drought is an alternative to decrease the negative effects of stresses on cowpea production. Thus, the objective of this work was to identify parents and combinations of cowpea genotypes with high probability of generating segregating populations with tolerance to drought. Six cowpea genotypes were crossed in a complete diallel cross design, totaling 30 F2 populations, which were evaluated together with their parents in an experiment under water deficit at the experimental field of the Embrapa Mid-North, Teresina, State of Piauí, Brazil. A triple lattice incomplete block experiment design was used, with three replications, with experimental plot consisting of six 2-m plant rows. Fifteen plants per plot were sampled to evaluate their agronomic characteristics, whose results were subjected to analysis of variance and means were used to estimate the general and specific combining abilities. The genotypes showed significant differences in all characteristics evaluated, denoting the genetic variability of the population. The additive effects were more important than the non-additive effects, and maternal inheritance was detected. The genotypes BRS Xiquexique, Pingo-de-Ouro-1-2 and MNC99-510F-16-1 were the most promising for use in recurrent selection programs for tolerance to water deficit. The hybrid combinations (1) BRS Paraguaçu X (4) CNCx-698-128G, (2) Pingo-de-Ouro-1-2 X (3) BRS Xiquexique, (3) BRS Xiquexique X (5) Santo-Inácio, (4) CNCx-698-128G X (6) MNC99-510F-16-1 and (5) Santo-Inácio X (4) CNCx-698-128G showed potential for generating superior lineages regarding bean production and tolerance to water deficit.

The aim of this study was to apply optical microscopy and scanning electron microscopy (SEM) techniques to identify and assess dripper clogging of an irrigation system. Three dripper models (Taldrip, Streamline, and Tiran) underwent 1200 hours of irrigation with three types of effluents: treated domestic wastewater, water with a high calcium content, and urban water supply. Samples of the material adhered to the inside of drippers were analyzed by means of the smear method aiming at their biological identification. Other samples were taken to obtain SEM images. The presence of algae, bacteria, and biological components of biofilm was observed in drippers operating with wastewater and water with calcium, this latter in a low number. The formation of biofilm and chemical precipitates of calcium were observed in SEM images, leading to emitter clogging. Optical microscopy and SEM analyses allowed identifying the degree of fouling by biofilm and chemical precipitates in dripper clogging.

This study aimed to evaluate the effects of chemical treatment of seeds with fungicide and insecticide on the seed physiological quality of Brachiaria brizantha cultivar MG5. Two experiments with four replicates were carried out in a completely randomized design. In the first experiment, the seeds were treated with the fungicide pyraclostrobin + fipronil + thiophanate-methyl and in the second, with the insecticide thiamethoxam, both at doses of 0, 150, 300, 450, and 600 mL of commercial product (CP)/100 kg of seeds. Physiological characterization was done on the basis of the first germination count, germination (%), emergence (%), emergence speed index, and length and dry mass of shoot and root. The treatment of B. brizantha seeds with the fungicide pyraclostrobin + fipronil + thiophanate-methyl benefits the physiological quality of seeds, improving germination and root development, with no phytotoxic effect up to the dose of 600 mL of CP/100 kg of seeds. The insecticide thiamethoxam has a biostimulating effect on B. brizantha cultivar MG5 up to the dose of 270 mL of CP/100 kg of seeds, but is phytotoxic in larger doses.