Copper pollutions are typical heavy metal contaminations, and their ability to move up food chains urges comprehensive studies on their effects through various pathways. Currently, four exposure pathways were prescribed as food-borne (FB), water-borne plus clean food (WCB), water–food-borne (WFB) and water-borne (WB). Caenorhabditiselegans was chosen as the model organism, and growth statuses, feeding abilities, the amounts of four antioxidant enzymes, and corresponding recovery effects under non-toxic conditions with food and without food were investigated. Based on analysis results, copper concentrations in exposure were significantly influenced by the presence of food and its uptake by C.elegans. Both exposure and recovery effects depended on exposure concentrations and food conditions. For exposure pathways with food, feeding abilities and growth statuses were generally WFB<WCB⩽FB (p<0.05). The antioxidant activities were up-regulated in the same order. Meanwhile, the exposure pathway without food (WB) caused non-up-regulated antioxidant activities, and had the best growth statuses. For recoveries with food, growth statuses, feeding abilities and the inductions of the antioxidant enzymes were all WB≈WFB<WCB<FB (p<0.05). For recoveries without food, the order of growth statuses remained WB>FB>WCB>WFB (p<0.05), while the antioxidant activities were all inhibited in a concentration–dependent fashion. In conclusion, contaminated food was the primary exposure pathway, and various pathways caused different responses of C.elegans.

The identification of CN– in water, seeds, and biological systems has, because of its high toxicity, attracted the increasing attention of many chemical industry researchers. In the work, a novel highly selective and reversible sensor, MMY, was shown to recognize CN– effectively. The color and fluorescent changes verified the interaction of MMY with CN–, and the fluorescence lifetime of MMY was also changed upon addition of CN–. A mode of interaction of MMY with CN– based on the results of various experiments was speculated. The LOD of MMY toward CN– was 9.4 × 10–¹⁰ M, lower than the concentration of CN– deemed acceptable by the WHO (World Health Organization) and the U.S. EPA (United States Environmental Protection Agency). MMY showed good reversibility and reusability for detecting CN–. In addition, test slips and silica plates were both earned by ourselves, which were able to recognize CN– qualitatively. Additionally, MMY could recognize CN– in tap water quantitatively with the use of a smartphone APP. Interestingly, MMY was also used to detect CN– in seeds. It was valid to image CN– in Caenorhabditis elegans and mice with a vivid “turn-on” fluorescence. MMY thus can circulate in the bloodstream.