Seasonal water-level fluctuations may lead to changes in river nutrients, which causes corresponding changes in the trophic structure of an aquatic food web, and finally affects the whole ecosystem. In this study, we focused on the Ganjing River, a tributary of the Yangtze River, China. Common organisms were sampled and measured for carbon and nitrogen stable isotopes in the wet and dry seasons, respectively, and the relative contributions of different food sources were combined to construct the food web, so as to realize the influence of water-level fluctuation on aquatic food web. Our results showed that basal food sources for fish consumers were endogenous carbon sources such as POM, zooplankton and zoobenthos in the dry season, while high water level exposed fish to more diverse and abundant food sources, and the contribution proportions of exogenous carbon sources (e.g., terrestrial detritus) to consumers increased in the wet season. In parallel, the abundance and species diversity of fish were higher than those in the dry season. Most fish species had relatively higher trophic levels in the dry season compared to the wet season, because the increase in fish densities led to an increase in piscivores fish. The food web was composed of planktonic and benthic food chains in the dry season. During the wet season, the planktonic food chain was dominant, followed by the herbivorous food chain, and the benthic food chain was relatively less important. Therefore, water-level fluctuation may alter the trophic linkages within fish communities, which contributed to a more complex and interconnected food web. Moreover, as we expect, the stable isotope analysis food web was broadly in line with the gut content analysis food web.

1. Growth and reproduction of Daphnia fed lake seston were measured in two categories of meso- to eutrophic lakes differing with respect to terrestrial organic matter influence (humic and clear water lakes). The content of highly unsaturated fatty acids (HUFA), P and N, as well as the taxonomical composition of seston were analysed. 2. Seston HUFA and C : P ratios were similar between lake categories, whereas C : N ratios were lower in the clear water lakes in both spring and summer. Despite the similarity in HUFA and P content of seston, Daphnia growth rate, clutch size and the proportion of gravid females were, respectively, about 1.5, 3 and 6 times higher in the clear water lakes. 3. Differences in growth and reproduction were related to a combination of higher N content and good fatty acid quality of the seston in the clear water lakes. Relatively high biomass of edible algae, such as Rhodomonas sp. and Cryptomonas sp., in the clear water lakes, and differences in water pH likely contributed to the observed differences in Daphnia growth and reproduction between lake categories. Additionally, it is possible that Daphnia was energy limited in the humic lakes despite high particulate organic carbon (POC) concentrations, as the contribution of non-algal and detrital C to the POC pool was high. 4. Our results suggest that dietary HUFA content has the potential to improve herbivore growth and reproduction if N and P are not limiting. N merits more attention in studies of zooplankton nutrition.

Plant water stress can affect selectivity by insect herbivores. Numerous studies have shown greater insect preference for water-stressed plants, but others have reported the opposite response. We evaluated leaf consumption by adults of Nyctelia circumundata (a chewing insect) in leaves of Larrea divaricata and Prosopis alpataco. Three bioassays (two-way choice tests) were performed: two intra-specific comparisons between well-watered (+W) and water-stressed (−W) leaves of each species and one inter-specific comparison between leaves of the two species. Leaf biomass was reduced by water stress in both species. Nitrogen concentration in leaves (N) was reduced by drought in P. alpataco. In contrast, total phenolics and specific leaf area (SLA) did not differ between treatments within species. Nyctelia circumundata did not show preference by any water supply regimes in intra-specific comparisons. In contrast, in inter-specific choice tests, it showed a marked preference for P. alpataco, which is the species with the highest nitrogen concentration and lowest total phenolics concentration. In intra-specific comparisons, maximum leaf consumption was inversely related to SLA in both species. Furthermore, in P. alpataco, N concentration was positively related to maximum leaf consumption and negatively related to leaf water content (LWC). In contrast, in inter-specific comparisons, total phenolics was negatively related to maximum leaf consumption, while N concentration exhibited the opposite trend. These results suggest that food selection is a hierarchical process where chemical attributes (i.e., total phenolics and N) are taken into account for species selection, and physical attributes (i.e., SLA and LWC) for choosing individuals inside species.

Much of our current knowledge about non-limiting dietary carbon supply for herbivorous zooplankton is based on experimental evidence and typically conducted at ~1 mg C L–¹ and ~20°C. Here we ask how low supply of dietary carbon affects somatic growth, reproduction, and survival of Daphnia magna and test effects of higher water temperature (+3°C relative to ambient) and brownification (3X higher than natural water color; both predicted effects of climate change) during fall cooling. We predicted that even at very low carbon supply (~5µg C L–¹), higher water temperature and brownification will allow D. magna to increase its fitness. Neonates (<24 h old) were incubated with lake seston for 4 weeks (October-November 2013) in experimental bottles submerged in outdoor mesocosms to explore effects of warmer and darker water. Higher temperature and brownification did not significantly affect food quality, as assessed by its fatty acid composition. Daphnia exposed to both increased temperature and brownification had highest somatic growth and were the only that reproduced, and higher temperature caused the highest Daphnia survival success. These results suggest that even under low temperature and thus lower physiological activity, low food quantity is more important than its quality for D. magna fitness.

Vertical mixing and stratification are among the most important physical processes controlling nutrient dynamics, the dominant category of primary producers and consequently the dominant types of food web, and are therefore important for the assessment of the marine ecosystem's response to global climate change. This study showed consistent short-term cyclic successions of the plankton food web types, governed by the dynamics of water column stability changes, occurring in generally oligotrophic, phosphate deficient surface waters of the open middle Adriatic Sea. The biogeochemical nitrogen cycle appeared as a key driving force responsible for the food web structure changes. The ‘herbivorous food web’ dominated during the nitrate-rich mixed water column period (winter) and gradually changed to ‘multivorous food web’ where large phytoplankton still constitute a significant fraction of phytoplankton. This intermediate type of food web lasted for a short time and quickly changed to the typical ‘microbial food web’, which then dominated during the stratified water column period (summer) and was characterised by a large proportion of picoplankton size-fraction organisms in total plankton biomass and production. Furthermore, at the very end of summer, the high bacterial carbon flux through the ‘microbial loop’ was established. The succession of food web types affects the mechanisms of bacterial control in a way that ‘bottom up’ control dominated during the mixed water column period and ‘top-down’ control prevailed during the stratified period. Since the ongoing global warming is expected to change water column stability dynamics and thereby significantly affect the supply of nutrients in surface waters, this study helps to understand the possible direction of changes in the plankton food webs of the Adriatic Sea, and consequent changes in marine nitrogen and carbon biogeochemical cycles.