The structured food systems (i.e. cellular tissues) are dissipative structures whose functionality mainly concerns their properties (physico-chemical properties, chemical and biochemical reactions), external interactions with surroundings (interactions with micro-organisms, heat and mass transport pathway) and especially, their interactions with consumers (nutritional value, quality, taste and flavour, texture, appearance: size, shape, colour). Dehydration or rehydration processes concern heat and mass transport phenomena (water, solutes) coupled with micro and macrostructure changes both producing important effects on food functionality. Control of these changes is the major concern in food product development. This control must be applied not only to the changes in physico-chemical properties but also to those related with consumers' issues. Food matrixengineering is a branch of food engineering which aims to apply the knowledge of the food matrixcomposition, structure and properties to promote and control adequate changes which can improve some sensorial and/or functional properties in the food. These changes, which are caused by some basic operations, are related to the phenomena of heat and mass transfer, vaporization-condensation, internal gas or liquid release, structure deformation-relaxation and phase transitions in matrixcomponents, and are usually coupled throughout the operation's progress. The final product may be a new product with improved composition and sensorial properties and/or more stability. All these concepts are discussed in this paper using several examples related to the application of combined food dehydration techniques.

A simple and rapid method for determination of carbofuran was developed using the fluorescence polarisation immunoassay (FPIA). Three tracers with different lengths of bridge (0-, 2- and 6-carbon bridge) between the hapten molecule 4-[[(2,3-dihydro-2,2-dimethyl-7-benzofuranyloxy)carbonyl]-amino]-butanoic acid (BFNB) and 5-aminofluorescein (AF), fluoresceinthiocarbamyl ethylenediamine (EDF), fluoresceinthiocarbamyl hexylenediamine (HDF), were synthesised and their binding response with anti-carbofuran-specific antibody were evaluated. The physicochemical parameters were optimised for the FPIA. The AF-labelled BFNB conjugate (BFNB-AF) was found to be the optimal tracer for FPIA of carbofuran. The detection limit of carbofuran, IC ₅₀ value and the working range were 2.3, 48.8 and 7.4−202.2 µg/L, respectively; and the reaction time was only 10 min. The average recovery from spiked water and vegetable samples was 86.9−95.4% and the mean coefficient of variation was 6.2% for inter-assay and 8.7% for intra-assay, which showed good reproducibility for FPIA. Thus, the developed FPIA method exhibited the potential for the rapid and accurate determination of carbofuran in agricultural and environmental samples.

There is increasing interest in the development of Coenzyme Q10 (CoQ10) ingredients appropriate for functional food formulations, due to its potential health effects. The present work reported that food proteins including milk and soy proteins can effectively perform as vehicles to remarkably improve water dispersibility, stability and even bioaccessibility of CoQ10. The improvement was mainly due to the formation of protein-CoQ10 complexes, through hydrophobic interactions. The formation of complexes with CoQ10 distinctly changed the physicochemical properties of food protein particles that seemed to be more favorable for their colloidal stability. The complexation remarkably improved the stability against UV exposure or in vitro digestion, and bioaccessibility of CoQ10. In contrast, milk proteins (sodium caseinate and whey protein concentrate) were more appropriate to perform as vehicles for improved bioaccessibility of CoQ10 than soy protein isolate. The findings provide an effective strategy to improve the delivery and bioaccessibility of CoQ10 for the formulation of functional foods enriched with CoQ10.

Engineered water nanostructures (EWNS) synthesized utilizing electrospray and ionization of water, have been, recently, shown to be an effective, green, antimicrobial platform for surface and air disinfection, where reactive oxygen species (ROS), generated and encapsulated within the particles during synthesis, were found to be the main inactivation mechanism. Herein, the antimicrobial potency of the EWNS was further enhanced by integrating electrolysis, electrospray and ionization of de-ionized water in the EWNS synthesis process. Detailed physicochemical characterization of these enhanced EWNS (eEWNS) was performed using state-of-the-art analytical methods and has shown that, while both size and charge remain similar to the EWNS (mean diameter of 13 nm and charge of 13 electrons), they possess a three times higher ROS content. The increase of the ROS content as a result of the addition of the electrolysis step before electrospray and ionization led to an increased antimicrobial ability as verified by E. coli inactivation studies using stainless steel coupons. It was shown that a 45-min exposure to eEWNS resulted in a 4-log reduction as opposed to a 1.9-log reduction when exposed to EWNS. In addition, the eEWNS were assessed for their potency to inactivate natural microbiota (total viable and yeast and mold counts), as well as, inoculated E. coli on the surface of fresh organic blackberries. The results showed a 97% (1.5-log) inactivation of the total viable count, a 99% (2-log) reduction in the yeast and mold count and a 2.5-log reduction of the inoculated E. coli after 45 min of exposure, without any visual changes to the fruit. This enhanced antimicrobial activity further underpins the EWNS platform as an effective, dry and chemical free approach suitable for a variety of food safety applications and could be ideal for delicate fresh produce that cannot withstand the classical, wet disinfection treatments.

Accurate measurement of water activity (aw) is an important goal for the food industry because aw is a key parameter in microbial growth, biological reaction rates and physical properties. An experimental device was setup using air-product water balance to non-destructively estimate the time-course of mean aw at the food product surface under well-controlled airflow conditions. The device is especially suited for studying the ripening of cheeses and fermented meat products, where water fluxes exchanged between products and air are very low. The validation tests performed with aw-known model products showed that water fluxes of 10(−7) kg s−1 can be estimated with an accuracy better than 2% over very short periods of time, and that surface aw can be estimated with an absolute uncertainty of less than 0.01 aw units. A handful of cheese ripening trials illustrate the potential of the method, highlighting the effects of a low air velocity and high air RH on the water exchanges occurring at a cheese surface, thus demonstrating the strong surface sensitivity to external air conditions.

In the meat industry there are some processes like drying or storage of salted meat products in which the knowledge of water sorption phenomena in salted proteins could be very useful. The sorption and desorption of most salted products is a singular process with three differentiated steps: a(w) < 0.75, a(w) = 0.75 and a(w) > 0.75. SAFES methodology allows the analysis of different elements in a system: the components, phases and states of aggregation in the food during the process to understand the process stages with a suitable level of complexity. It also analyzes the transport functions, chemical reactions and the phenomena occurring during the processing of the product. The aim of this paper is to analyze the sorption phenomena of water in salted proteins using the SAFES methodology for the three different steps of the water desorption process. Salted pork meat isotherms at different three different salt concentrations and three various temperatures were analyzed in order to observe differences between them, in terms of mass transport, reactions, etc. With SAFES methodology, differences in the behaviour of the system, depending on the amount of NaCl added to the pork meat were observed. Differences in mass fluxes were found in relation to temperature and NaCl concentration.

Despite the progress in the area of food safety, foodborne diseases still represent a massive challenge to the public health systems worldwide, mainly due to the substantial inefficiencies across the farm-to-fork continuum. Here, we report the development of a nano-carrier platform, for the targeted and precise delivery of antimicrobials for the inactivation of microorganisms on surfaces using Engineered Water Nanostructures (EWNS). An aqueous suspension of an active ingredient (AI) was used to synthesize iEWNS, with the ‘i’ denoting the AI used in their synthesis, using a combined electrospray and ionization process. The iEWNS possess unique, active-ingredient-dependent physicochemical properties: i) they are engineered to have a tunable size in the nanoscale; ii) they have excessive electric surface charge, and iii) they contain both the reactive oxygen species (ROS) formed due to the ionization of deionized (DI) water, and the AI used in their synthesis. Their charge can be used in combination with an electric field to target them onto a surface of interest. In this approach, a number of nature-inspired antimicrobials, such as H₂O₂, lysozyme, citric acid, and their combination, were used to synthesize a variety of iEWNS-based nano-sanitizers. It was demonstrated through foodborne-pathogen-inactivation experiments that due to the targeted and precise delivery, and synergistic effects of AI and ROS incorporated in the iEWNS structure, a pico-to nanogram-level dose of the AI delivered to the surface using this nano-carrier platform is capable of achieving 5-log reductions in minutes of exposure time. This aerosol-based, yet ‘dry’ intervention approach using iEWNS nano-carrier platform offers advantages over current ‘wet’ techniques that are prevalent commercially, which require grams of the AI to achieve similar inactivation, leading to increased chemical risks and chemical waste byproducts. Such a targeted nano-carrier approach has the potential to revolutionize the delivery of antimicrobials for sterilization in the food industry.

The U.S. Food and Drug Administration (FDA) has defined standards for the microbial quality of agricultural surface water used for irrigation. According to the FDA produce safety rule (PSR), a microbial water quality profile requires analysis of a minimum of 20 samples for Escherichia coli over 2 to 4 years. The geometric mean (GM) level of E. coli should not exceed 126 CFU/100 mL, and the statistical threshold value (STV) should not exceed 410 CFU/100 mL. The water quality profile should be updated by analysis of a minimum of five samples per year. We used an extensive set of data on levels of E. coli and other fecal indicator organisms, the presence or absence of Salmonella, and physicochemical parameters in six agricultural irrigation ponds in West Central Florida to evaluate the empirical and theoretical basis of this PSR. We found highly variable log-transformed E. coli levels, with standard deviations exceeding those assumed in the PSR by up to threefold. Lognormal distributions provided an acceptable fit to the data in most cases but may underestimate extreme levels. Replacing censored data with the detection limit of the microbial tests underestimated the true variability, leading to biased estimates of GM and STV. Maximum likelihood estimation using truncated lognormal distributions is recommended. Twenty samples are not sufficient to characterize the bacteriological quality of irrigation ponds, and a rolling data set of five samples per year used to update GM and STV values results in highly uncertain results and delays in detecting a shift in water quality. In these ponds, E. coli was an adequate predictor of the presence of Salmonella in 150-mL samples, and turbidity was a second significant variable. The variability in levels of E. coli in agricultural water was higher than that anticipated when the PSR was finalized, and more detailed information based on mechanistic modeling is necessary to develop targeted risk management strategies.

Fracture stress of soybean curd was measured at temperatures between -20 degrees C and -196 degrees C by compression tests. Fracture stress increased as the temperature decreased until it reached a characteristic temperature; below this temperature the fracture stress was constant. Fracture stress varied with the moisture content of the sample measured before freezing; the lower the moisture content, the higher the fracture stress. We attempted to analyze the fracture stress of the frozen soybean curd using a mathematical model in which frozen soybean curd was regarded as a two-component system consisting of pure water ice and concentrated amorphous solution (CAS). Since the volumetric fraction of pure water ice in the system is required for the analysis, the degree of freezing of soybean curd with varied moisture content was estimated as a function of temperature using a hypothetical phase diagram for soybean curd. Based on these data, fracture stress of CAS was calculated using a series model and a parallel model. The calculated fracture stress of CAS was found to be a unique function of temperature and independent of moisture content before freezing.

Food processing waste waters at two irrigated land disposal sites and subsurface waters (perched and ground waters) were monitored at daily to monthly intervals over one annual cycle of production. Soil profiles were sampled to depths up to 6.6 m in the early fall. Yearly inputs were calculated at 487 kg/ha total N (Kjeldahl plus NO³-N) and 101 kg/ha soluble PO₄-P (orthophosphate) from cannery wastes at site 1. Estimates for milk wastes at site 2 were 562 kg/ha total N and 522 kg/ha PO₄-P. The range for NO₃-N in subsurface waters was 7 to 16 ppm at site 1 (perched water at 1.5 m) and 2 to 41 ppm at site 2 (ground water at 0.9 m). Maximum concentrations, found in summer, were essentially the same as the average for total N in the input wastes (16 ppm at site 1 and 38 ppm at site 2). Nitrate was stable in the percolation stream below the root zone. Annual additions to subsurface waters were estimated at 76% of input N at site 1 and 65% at site 2. The range of PO₄-P in subsurface waters was 0.5 to 1.5 ppm at site 1 and 0.04 to 1.8 ppm at site 2; average waste water concentrations were 3 and 35 ppm. The highest concentrations in subsurface water were found in spring. Annual subsurface discharge was estimated at 27% of input P at site 1 and 2% at site 2. The extensive removals of PO₄ and the similar concentrations encountered in subsurface waters are of theoretical and practical interest since PO₄-P had already accumulated in soil profiles at both sites in quantities which exceed the Langmuir maxima for nonirrigated control soils. During seasons of major irrigation input, NO₃ appeared in subsurface waters in concentrations exceeding public health standards; PO₄ concentrations exceeded environmental guidelines at all times except where irrigation was discontinued during the winter at site 2. Soil systems appeared poised to discharge at the observed rates because of the large quantities of organic N and fixed P which had accumulated in the profiles over 20 years operation at site 1, and 10 years at site 2. The rate of residual accumulation in soil could have been reduced by harvest, to extend system life materially. The harvest potential of three grass clippings per season removed for silage, was estimated experimentally at 31% of input N at both sites and 80% of input PO₄ at site 1; 27% at site 2.