Numerous studies have shown that intestinal and urinary tract flora are closely related to the formation of kidney stones. The removal of probiotics represented by lactic acid bacteria and the colonization of pathogenic bacteria can directly or indirectly promote the occurrence of kidney stones. However, currently existing natural probiotics have limitations. Synthetic biology is an emerging discipline in which cells or living organisms are genetically designed and modified to have biological functions that meet human needs, or even create new biological systems, and has now become a research hotspot in various fields. Using synthetic biology approaches of microbial engineering and biological redesign to enable probiotic bacteria to acquire new phenotypes or heterologous protein expression capabilities is an important part of synthetic biology research. Synthetic biology modification of microorganisms in the gut and urinary tract can effectively inhibit the development of kidney stones by a range of means, including direct degradation of metabolites that promote stone production or indirect regulation of flora homeostasis. This article reviews the research status of engineered microorganisms in the prevention and treatment of kidney stones, to provide a new and effective idea for the prevention and treatment of kidney stones.

The inactivation of microorganisms is critical for ensuring the safety of food products. Currently, emerging electrotechnologies have attracted considerable attention. These non-conventional technologies for food processing rely on thermal effects, non-thermal effects, or a combination of them to achieve this goal. Conventional pasteurization requires a heat transfer medium. It is often performed at high temperatures, leadinging to food quality degradation and flavour distortion. Electrotechnologies contain ohmic heating (OH), pulsed electric field (PEF), and induced electric field (IEF) treatment, which utilize both electric potential difference and current. Compared to conventional heating, they can reduce inactivation temperature thresholds and duration while retaining nutritional value, having a minimal negative impact on food quality, flavor, and color. Since the main purpose of food processing is based on microorganism inactivation to ensure food safety and storability, it is worthwhile to pay attention to the current application of electric field technologies. This paper reviews the research progress on the inactivation of microorganisms by OH, PEF, and IEF treatment. Additionally, it presents the generation characteristics of electrotechnologies and an outlook on future development.

Probiotics are live microorganisms that, when consumed in adequate amounts, exert health benefits on the host by regulating intestinal and extraintestinal homeostasis. Common probiotic microorganisms include lactic acid bacteria (LAB), yeasts, and Bacillus species. Here, we present Probio-ichnos, the first manually curated, literature-based database that collects and comprehensively presents information on the microbial strains exhibiting in vitro probiotic characteristics (i.e., resistance to acid and bile, attachment to host epithelia, as well as antimicrobial, immunomodulatory, antiproliferative, and antioxidant activity), derived from human, animal or plant microbiota, fermented dairy or non-dairy food products, and environmental sources. Employing a rigorous methodology, we conducted a systematic search of the PubMed database utilizing the keyword ‘probiotic’ within the abstracts or titles, resulting in a total of 27,715 studies. Upon further manual filtering, 2207 studies presenting in vitro experiments and elucidating strain-specific probiotic attributes were collected and used for data extraction. The Probio-ichnos database consists of 12,993 entries on the in vitro probiotic characteristics of 11,202 distinct strains belonging to 470 species and 143 genera. Data are presented using a binary categorization approach for the presence of probiotic attributes according to the authors’ conclusions. Additionally, information about the availability of the whole-genome sequence (WGS) of strains is included in the database. Overall, the Probio-ichnos database aims to streamline the navigation of the available literature to facilitate targeted validation and comparative investigation of the probiotic properties of the microbial strains.

Selenium (Se) is a vital trace element integral to numerous biological processes in both plants and animals, with significant impacts on soil health and ecosystem stability. This review explores how soil microorganisms facilitate Se transformations through reduction, oxidation, methylation, and demethylation processes, thereby influencing the bioavailability and ecological functions of Se. The microbial reduction of Se compounds, particularly the conversion of selenate and selenite to elemental Se nanoparticles (SeNPs), enhances Se assimilation by plants and impacts soil productivity. Key microbial taxa, including bacteria such as Pseudomonas and Bacillus, exhibit diverse mechanisms for Se reduction and play a substantial role in the global Se cycle. Understanding these microbial processes is essential for advancing soil management practices and improving ecosystem health. This review underscores the intricate interactions between Se and soil microorganisms, emphasizing their significance in maintaining ecological balance and promoting sustainable agricultural practices.

The variation in the population of pathogenic microorganisms in wastewater over time can be predicted by single-spectral analysis, which outperforms conventional multivariate methods. This type of information is important for the performance of sewage treatment plants. The objective of this study was to monitor the variability of the population of microorganisms and the removal of microbiological attributes of sanitary wastewater in a treatment plant equipped with a septic tank and solar reactor. Effluent samples were collected monthly upstream of the septic tank and inside the solar reactor, and the effluent was exposed to solar radiation. Total coliforms and <i>Escherichia coli</i> were analyzed by means of descriptive analysis and singular spectrum analysis. Solar disinfection obtained bacterial inactivation levels of 99.94%, equivalent to 4 log units for the <i>Escherichia coli</i> population, and 99.45%, equivalent to 3 log units for the total coliform population. For the inlet effluent, the prediction showed a trend of growth alternating with periods of stability. For the outlet effluent, the prediction was able to provide regular data up to the first six months, showing error and overestimation of the data for the final six months of the study.

In the European Union and worldwide there are a burgeoning markets for plant growth promoting microorganisms (PGPM) and other biological agents as soil improvers, bio-fertilizers, plant bio-stimulants, and biological control agents or bio-pesticides. Microbial agents have a major share in this development. The use of such products is often advertised with the promise of contributing to sustainable agricultural practices by increasing crop growth and yield and offering an alternative or substitute to decrease the dependency of agriculture on hazardeous agrochemicals. In contrast to registered microbial plant protection products, PGPM that are marketed in the EU as soil improvers or plant biostimulants, are not strictly required to have proven minimum efficacy levels under field conditions. Manufacturers only have to ensure that these products do not pose unacceptable risks to human, animal or plant health, safety or the environment. Uniform guidelines comparable to the EPPO - standards (European and Mediterranean Plant Protection Organisation) to test the efficacy in field trials are not available. This paper attempts to fill the gap. It proposes guidelines for PGPM field trial design and implementation, as well as recommendations for the type and scope of data collection and evaluation. Selected research papers from literature were evaluated to analyze, whether and to what extent the requirements are already met. The majority of the papers had a clear experimental design followed by proper data evaluation. Frequent deficiencies were the low number of tested environments and crop species, insufficient site and agronomic management description and missing data on soil humidity and temperature. Using the suggested standards is assumed to increase the expressive power of tested microbial products.

Pathogenic microorganisms can spread in the air as bioaerosols. When the human body is exposed to different bioaerosols, various infectious diseases may occur. As indoor diagnosis and treatment environments, hospitals are relatively closed and have a large flow rate of people. This indoor environment contains complex aerosol components; therefore, effective sampling and detection of microbial elements are essential in airborne pathogen monitoring. This article reviews the sampling and detection of different kinds of microorganisms in bioaerosols from indoor diagnostic and therapeutic settings, with a particular focus on microbial activity. This provides deeper insights into bioaerosols in diagnostic and therapeutic settings.

Methods of molecular genetic analysis are widely used to study the air microbiota. These methods make it possible to study all groups of microorganisms without the need for their cultivation. The purpose of the study was to analyze the microbiota of the poultry house air environment by the qPCR method and identify the presence of representatives of the normophlora, opportunistic and pathogenic microflora. The research plan included sampling of the air microbiota from a three-tier cage battery for poultry rearing for 42 days. The data obtained revealed an increase in the presence of microorganisms during the entire experiment, the largest number of them was detected on the 42nd day of cultivation on the lower tier. The results of the study emphasize the importance of timely detection and assessment of the microbiota of the air to prevent the spread of diseases and their impact on the health and productivity of birds.

Foodborne pathogen contamination of fermented food is an increasingly concerned problem in food industry. The types and sources of harmful microorganisms in food are discussed in this paper. The main techniques used to detect microorganisms are summarized. In addition, strategies for controlling these harmful microorganisms are discussed. By highlighting these important aspects, this paper aims to contribute to strengthening food quality assurance and promoting safer food practices.