Nonwoven materials as a supporting agent for in vitro culture of banana protocorm-like bodies
1989
Matsumoto, K. | Yamaguchi, H. (Tokyo Univ., Bunkyo-ku (Japan). Fac. of Agric. Dept. of Agrobio. Lab. of Radiation Genetics and Chemical Mutagenesis)
The use of synthetic unwoven materials as a support in culture technique to substitute for agar gelling media in plant tissue culture was studied. Banana protocorm-like bodies (PLBs) were cultured on the nonwoven materials: polyester, SS-400, AT-400, absorbent cotton and Toyobo-Cloud, which absorbed a liquid medium. AT-400 (acetate 50 % and polyester 50 %) was the best material tested in terms of PLB growth and reusability. This culture technique should be useful for the in vitro propagation and the selection of mutants
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