Application of immunoblotting for chromosomal localization of non-enzymic protein structural genes: alpha-amylase inhibitor gene of rye
1988
Sadowski, J. (Polish Academy of Sciences, Poznan (Poland). Inst. of Plant Genetics) | Daussant, J. (Centre National de Recherche Scientifique, Meudon (France). Lab. de Physiologie des Organes Vegetaux)
This paper describes a study on the chromosomal localization of structural gene (s) coding for alpha-amylase inhibitor in rye with the use of a direct and very sensitive technique - immunoblotting carried out after isoelectric focusing. Wheat-rye addition lines were used for the gene chromosomal localization. Structural gene (s) coding for alpha-amylase inhibitor were localized on chromosome 2, confirming earlier results obtained by indirect techniques. The present results illustrate the usefulness of immunoblotting in genetic studies dealing with non-enzymic proteins
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