Preparation of maize cDNA
1992
Cernakova, M. (Slovenska Technicka Univ., Bratislava (Slovak Republic). Chemickotechnologicka Fakulta)
This work deals with the preparation of the gene bank for the cDNA maize. At first mtRNA, a matrix for cDNA synthesis, was isolated. For cDNA synthesis four dNTP, oligonucleotide primer were necessary. Reverse transcriptase is a key-enzyme that mediates transcription from mtRNA to ss-DNA. The gene bank was prepared by the cDNA synthesis. The maize genome transferred into a different gene bank using a suitable vector, may be the subject of studying the control system of zein proteins. It can also be used for cloning (multiplication) of the gene for lysine production.
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