A simple protocol for rapid and efficient isolation of protoplast from callus cultures of mulberrry (Morus indica L.) cv. S13 | Un protocole simple pour l'isolement de protoplastes rapide et efficace à partir de cultures de cals de mûrier (Morus indica L.) du cv. S13
2000
Pavan, U. ((Kakatiya University, Warangal (Inde). Department of Botany, Plant Biotechnology Resarch Unit)) | Rao, A.V. | Yashodhara, V. | Rama Swamy, N. | Sadanandam, A.
Callus cultures were initiated from mature nodal segments of mulberry (Morus indica L.) cultivar S13 on Murashige and Skoog (MS) medium supplemented with 1 mg/l 2,4,5-trichlorophenoxy acetic acid (2,4,5-T). Protoplasts were isolated from 20 days old callus cultures incubated in digestion solution containing 0.25 / of macerozyme R-10 and 1 / of cellulase "Onozuka" R-10 after 4h of digestion. Furthermore, a new procedure is developed for isolation and purification of mulberry callus protoplasts
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