Integrated strategy for the qualitative and quantitative analysis of residues of antimicrobial substances in food products
2001
Pieckova, E. (Ustav Preventivnej a Klinickej Mediciny, Bratislava (Slovak Republic)) | van Peteghem, C. H.
An efficient drug residue monitoring should involve screening as well as confirmatory methods. A demonstration of the feasability of an integrated strategy for the qualitative and quantitative analysis of residues of antibacterial substances in foodstuffs of animal origin (edible tissues) was aimed by the running project. Screening methods - high performance thin layer chromatography for sulfonamide residues and enzymatic immunoassays for tetracyclines and aminoglycosides (neomycin), followed by confirmation by means of high performance liquid chromatography with mass-spectrometric detection, were carried out. The screening methods used were all simple and relatively low-cost, and provided reliable results. Out of the samples tested, 16, 81, 13% of kidney and 31, 75, 9% of muscle samples were positive for tetracyclines, sulfonamides or neomycin
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