Utilization of various methods of cytotoxicity detection in aquatic toxicology

Kolarova, J. . Vyzkumny Ustav Rybarsky a Hydrobiologicky; Schmidtova, M.

Utilization of various methods of cytotoxicity detection in aquatic toxicology

2001

Kolarova, J. (Jihoceska Univ., Vodnany (Czech Republic). Vyzkumny Ustav Rybarsky a Hydrobiologicky) | Schmidtova, M.

Three assays were used for checking the cytotoxic effect of a model substance (K2Cr2O7) to the fish cell lines EPC, RTG and FHM. The assays were used as follows: NR assay based on the principle of accumulation of neutral red dye in lysozomes of animal cells, MTT assay detecting live cells by means of the activity of mitochondrial dehydrogenase; and Cell Death Detection ELISA for checking the apoptosis by determining the amount of DNA fragments in cell cytoplasm. Our results lead to the hypothesis that the first toxic effects of potassium dichromate affect the mitochodrial apparatus and trigger apoptotic changes.

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Mots clés AGROVOC

apoptose apoptosis cell culture cells cellule culture de cellule elisa ensayo epitelio epithelium in vitro experimentation inorganic acid salts methode methods potasio potassium test elisa testage testing toxicidad toxicity

Informations bibliographiques

Editeur

Jihoceska Univerzita

Pagination

p. 222-232

D'autres materias

Experimentation in vitro; Cultivo de celulas; Metodos; Celulas; Toxicite; Sel d'acide mineral; Experimentacion in vitro; Sales de acidos inorganicos

Langue

tchèque

Note

Summary (En)

7 graphs; 4 ref.

ISBN

80-85887-43-6

Titre traduit

Vyuziti ruznych metod detekce cytotoxicity ve vodni toxikologii

Type

Summary; Conference; Non-Conventional

Source

Toxicity and biodegradability of matters important in water management. Proceedings of the 10. international conference.- Vodnany (Czech Republic): Jihoceska Univerzita, 2001.- ISBN 80-85887-43-6. 239 p.

Conférence

Toxicity and biodegradability of matters important in water management, Ceske Budejovice (Czech Republic), 20-22 Aug 2001

Sur AGRIS depuis: 2003-08-15

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Utilization of various methods of cytotoxicity detection in aquatic toxicology

2001

Mots clés AGROVOC

Informations bibliographiques