Screening of lipase-producing bacteria from rotten oil palm fruits
1998
Jantana Jinda, University of the Thai Chamber of Commerce, Bangkok (Thailand). Faculty of Science
56 isolated bacteria during growth on rotten oil palm fruits were studied on olive oil emulsion agar (olive oil 2 percent) and crude palm oil emulsion agar (crude palm oil 2 percent). 21 lipase-producing bacteria isolates can hydrolise olive oil in olive oil emulsion agar, only. Crude palm oil in crude palm oil emulsion agar was by 5 isolates. And both olive oil and crude palm oil were hydrolised by 17 isolates of lipase-producing bacteria. Three groups of selected bacterial were inoculated on Trybutyrin emulsion agar for 24 hours to clear zone around its colony testing and 7 isolates that showed 1.8-2.0 cm width of clear zone were choosen. Only 3 isolates, UTCC-B20, UTCC-B55 and UTCC-B56 produced extracellular lipase activity of 2,093.8, 1,666.7 and 2,708.3 U/L, respectively after culture at 37 deg C for 24 hour in a media containing 10 percent (v/v) base mixture, 0.01 percent yeast extract, and 1 percent olive oil in 0.2 M phosphate buffer pH 7.0.
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