Effects of Ethanol on Secretion of Insulin-like Growth Factor-I (IGF-I) and Insulin-like Growth factor Binding Protein-1 (IGFBP-1) in primary cultured rat Hepatocytes: Involvement of Lipid Peroxides (LPO) Activity
2004
Lee, S.M. (Chonbuk National University, Jeonju, Republic of Korea) | Kang, C.W. (Chonbuk National University, Jeonju, Republic of Korea), E-mail: [email protected]
In the present study, we evaluated the effects of ethanol on insulin like growth factor (IGF)-I and insulin like growth factor binding protein (IGFBP)-1 secretion using RT-PCR, western immunoblotting and radio-immuno assay (RIA) in primary cultured rat hepatocytes. IGF-I secretion and gene expression with different dosages of ethanol (1, 5, 10, 50 and 100 mM) were suppressed in dose-dependent manner.
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