Modeling the activity of selected lysosomal enzymes in the liver and kidney of laboratory mice supplementation of vitamin E and different levels of dietary protein in diets
2010
Kumanski, K. | Jozwiak, J. | Jedras, M.
For the purpose of our experiment a model of reaction to stress on the level of cellular organelle was implemented. In this model the activity of chosen enzymes was measured in lysosomal, microsomal and cytosolic fraction of centrifuged homogenate of liver and kidney of Swiss laboratory mice. The results were assessed by means of three-way analysis of variance by SAS system and proved to be of high statistical significance. Yet at this point, though alpha tocopherol created spectacular multidirectional changes in enzyme activity, further focused studies are required to establish the extent to which antioxidative, non-antioxidative or perhaps membrane destabilizing mechanism are responsible. The results of our experiment suggest certain practical implication and new proposition of interpretation of enzymatic model of cellular reaction against oxidative stress
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