Evidence that more than 90% of beta-glucuronidase-expressing cells after particle bombardment directly receive the foreign gene in their nucleus.
1991
Yamashita T. | Iida A. | Morikawa H.
Plasmid DNA harboring the beta-glucuronidase (GUS) gene, coated on gold particles, was delivered into cultured tobacco (Nicotiana tabacum L. cv Bright Yellow-2) cells using a pneumatic particle gun. Cytological analyses of intracellular location of the introduced gold particles before and after GUS-expression assay indicated that more than 90% of GUS-expressing cells after bombardment received a DNA-coated particle in their nucleus.
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