Evidence that more than 90% of beta-glucuronidase-expressing cells after particle bombardment directly receive the foreign gene in their nucleus.
1991
Yamashita T. | Iida A. | Morikawa H.
Plasmid DNA harboring the beta-glucuronidase (GUS) gene, coated on gold particles, was delivered into cultured tobacco (Nicotiana tabacum L. cv Bright Yellow-2) cells using a pneumatic particle gun. Cytological analyses of intracellular location of the introduced gold particles before and after GUS-expression assay indicated that more than 90% of GUS-expressing cells after bombardment received a DNA-coated particle in their nucleus.
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书目信息
页码
v.829-831(2)
其它主题
Expresion genica; Actividad enzimatica; Transfert de gene; Expression des genes; Activite enzymatique; Cultivo de celulas
语言
英语
注释
references. AVAILABILITY: US (DNAL 450 P692).
类型
Journal Article; Journal Part
来源
Plant-physiology (USA). (Oct 1991). v. 97(2) p. 829-831.
团体作者
Hiroshima University, Hiroshima, Japan
2012-11-15
2026-02-03
AGRIS AP