Characterization and translation studies of potato virus S RNA.

Monis J.; De Zoeten G.A.

Characterization and translation studies of potato virus S RNA.

1990

Monis J. | De Zoeten G.A.

The Mr of the Andean (An) strain of potato virus S (PVS) RNA was determined to be 2.4 X 10(6) (7.5 kilobases [kb]) when assayed by electrophoresis under denaturing conditions. Oligo d(T) cellulose chromatography and direct RNA sequencing demonstrated that viral RNA is polyadenylated. In vitro translation in rabbit reticulocyte lysates indicates that four major protein products of 140K, 123K, 104K, and 34K are produced by PVS-An RNA. Antisera specific to the Andean and type strains of PVS immunoprecipitated a major protein product of 34K. Infection of potato protoplasts indicated the production of a polypeptide of 34K, which was not detected in mock inoculated protoplasts and reacted specifically with PVS-AN antisera in immunoprecipitation experiments.

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Mots clés AGROVOC

arn electroforesis electrophoresis genetic transformation immunoprecipitation tests plant viruses purification rna solanum tuberosum virus de las plantas

Informations bibliographiques

Publié dans

Phytopathology

Volume 80 ISSN 0031-949X

Pagination

v.441-445(5)

D'autres materias

Transformation genetique; Epuration; Electrophorese; Purificacion; Reaccion de inmunoprecipitacion; Reaction d' immunoprecipitation; Virus des vegetaux; Transformacion genetica

Langue

anglais

Note

ill. references.

Type

Journal Article; Journal Part

Source

Phytopathology (USA). (May 1990). v. 80(5) p. 441-445.

Auteur institutionnelle

University of Texas, Dallas

Centre de Cooperation Internationale en Recherche Agronomique pour le Developpement, Reunion (France). IRAT. Institut de Recherches Agronomiques Tropicales et des Cultures Vivrieres, Laboratoire de Phytopathologie. Ecole Nationale Superieure Agronomique, Montpellier (France). Centre National d' Etudes Agronomiques des Regions Chaudes, Montpellier (France). ESAT. Ecole Superieure d' Agronomie Tropicale.

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