Characterization and translation studies of potato virus S RNA.
1990
Monis J. | De Zoeten G.A.
The Mr of the Andean (An) strain of potato virus S (PVS) RNA was determined to be 2.4 X 10(6) (7.5 kilobases [kb]) when assayed by electrophoresis under denaturing conditions. Oligo d(T) cellulose chromatography and direct RNA sequencing demonstrated that viral RNA is polyadenylated. In vitro translation in rabbit reticulocyte lysates indicates that four major protein products of 140K, 123K, 104K, and 34K are produced by PVS-An RNA. Antisera specific to the Andean and type strains of PVS immunoprecipitated a major protein product of 34K. Infection of potato protoplasts indicated the production of a polypeptide of 34K, which was not detected in mock inoculated protoplasts and reacted specifically with PVS-AN antisera in immunoprecipitation experiments.
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