A simple procedure for rehybridization of nuclei analyzed previously by fish
1999
Carvalho-Salles Andréa Borduchi | Barril Nilce | Tajara Eloiza Helena
We present a simple procedure for interphase fluorescence in situ rehybridization (FISH). This procedure was used to evaluate fresh prostate tumor from needle biopsy specimens. Digoxigenin-labeled DNA probes were hybridized onto nuclei which had been previously investigated by FISH using biotin-labeled DNA probes. This method makes it possible to reanalyze the same nuclei with different centromeric DNA probes and is useful in cases where a limited number of slides are available.
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