A simple procedure for rehybridization of nuclei analyzed previously by fish

Carvalho-Salles Andréa Borduchi; Barril Nilce; Tajara Eloiza Helena

A simple procedure for rehybridization of nuclei analyzed previously by fish

1999

Carvalho-Salles Andréa Borduchi | Barril Nilce | Tajara Eloiza Helena

We present a simple procedure for interphase fluorescence in situ rehybridization (FISH). This procedure was used to evaluate fresh prostate tumor from needle biopsy specimens. Digoxigenin-labeled DNA probes were hybridized onto nuclei which had been previously investigated by FISH using biotin-labeled DNA probes. This method makes it possible to reanalyze the same nuclei with different centromeric DNA probes and is useful in cases where a limited number of slides are available.

显示更多 [+]