Effects of mixed seed soaking on germination and seedling growth of foxtail millet (Setaria italica) under drought stress | 复配浸种对干旱胁迫下谷子萌发及幼苗生长的影响

chinois. 以晋谷21号为试材, 以含水量为9%的土壤模拟干旱胁迫, 通过对谷子发芽率、发芽势、株高及渗透调节物质、膜脂过氧化、活性氧和保护酶活性等指标进行测定分析, 研究GA3、稀土和脯氨酸复配浸种后在干旱胁迫下谷子萌发及幼苗生理特性的影响。结果表明, 混合药剂浸种处理能够缓解干旱胁迫对种子萌发的抑制, 提高种子发芽率和发芽势; 同时提高谷子脯氨酸和可溶性蛋白含量, 降低渗透势, 促进种子吸水; 诱导抗氧化系统相关酶活性上升; 抑制干旱胁迫下谷子幼苗叶片O2-.的产生速率和H2O2的积累, 降低MDA含量。说明GA3、稀土以及脯氨酸复配浸种能够提高谷子叶片抗氧化能力,减轻干旱胁迫诱导的膜脂过氧化损伤并改善干旱胁迫下谷子幼苗的渗透调节, 从而提高谷子幼苗对干旱胁迫的适应性。

anglais. In the study, the foxtail millet (Setaria italica) (Jingu 21) seeds were treated with the mixed medicament soaking of gibberellin, rare earth and proline for 10 min. The treated seeds were sowed in the soil of 9% water content and the germination rate, germination potential, plant height and osmotic regulation substances, membrane lipid peroxidation, reactive oxygen species and protective enzyme activity were determined. The results showed that seed germination rate and germination potential could be increased and seed germination inhibition were alleviated under drought stress. The contents of proline and soluble protein in the seeds were increased, the osmotic potential was reduced, while the water absorption in seed was promoted. In the millet seedling, the activities of anti-oxidant enzymes were increased and production rate of O-2_and H2O2 decreased. The degree of membrane lipid peroxidation was reduced by the soaking treatment under drought stress. In conclusion, after the millet seeds were treated with the mixed soaking, drought stress adaptability were improved by the increase of antioxidant capacity and osmotic adjustment of leaves and reduction of membrane lipid peroxidation injury.