Phage PVP-SE1 as Tool Recognition in Polydiacetylene to Produce Intelligent Packaging
2017
Taila V., de Oliveira | Nilda de F. F., Soares | Carla M., Carvalho | Pablo, Fuciños | Jane S. dos R., Coimbra | Nélio J. de, Andrade | Eber A. A., Medeiros | Paulo P., Freitas
Polydiacetylene (PDA) compound has unique chromatic properties when embedded in methylcellulose base. Therefore, this could be used as intelligent packaging. PDA exhibits blue-to-red color transition under certain stimuli, which was caused by alterations in PDA conjugation length backbone. For a specific color modification, bio-recognition tools were covalently linked with PDA. In this work, a broad-spectrum virulent phage (PVP-SE1) was used as a biorecognition element to detect Salmonella cells due to PDA color change. A series of experiments were performed to monitor colorimetric response. Consequently, chromatic immunoassay could be significant in increasing potential applications of PDA intelligent packaging.
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