Phage PVP-SE1 as Tool Recognition in Polydiacetylene to Produce Intelligent Packaging

Taila V., de Oliveira; Nilda de F. F., Soares; Carla M., Carvalho; Pablo, Fuciños; Jane S. dos R., Coimbra; Nélio J. de, Andrade; Eber A. A., Medeiros; Paulo P., Freitas

Phage PVP-SE1 as Tool Recognition in Polydiacetylene to Produce Intelligent Packaging

2017

Polydiacetylene (PDA) compound has unique chromatic properties when embedded in methylcellulose base. Therefore, this could be used as intelligent packaging. PDA exhibits blue-to-red color transition under certain stimuli, which was caused by alterations in PDA conjugation length backbone. For a specific color modification, bio-recognition tools were covalently linked with PDA. In this work, a broad-spectrum virulent phage (PVP-SE1) was used as a biorecognition element to detect Salmonella cells due to PDA color change. A series of experiments were performed to monitor colorimetric response. Consequently, chromatic immunoassay could be significant in increasing potential applications of PDA intelligent packaging.

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