An interlaboratory comparison of physiological and genetic properties of four Saccharomyces cerevisiae strains
2000
Dijken, J.P. van | Bauer, J. | Brambilla, L. | Duboc, P. | Francois, J.M. | Gancedo, C. | Giuseppin, M.L.F. | Heijnen, J.J. | Hoare, M. | Lange, H.C. | Madden, E.A. | Niederberger, P. | Nielsen, J. | Parrou, Jean-Luc | Petit, T. | Porro, D. | Reuss, M. | Van Riel, N | Rizzi, M. | Steensma, H.Y. | Verrips, C.T. | Vindeløv, J. | Pronk, J.T.
To select a Saccharomyces cerevisiae reference strain amenable to experimental techniques used in (molecular) genetic, physiological and biochemical engineering research, a variety of properties were studied in four diploid, prototrophic laboratory strains. The following parameters were investigated: 1) maximum specific growth rate in shake-flask cultures; 2) biomass yields on glucose during growth on defined media in batch cultures and steady-state chemostat cultures under controlled conditions with respect to pH and dissolved oxygen concentration; 3) the critical specific growth rate above which aerobic fermentation becomes apparent in glucose-limited accelerostat cultures; 4) sporulation and mating efficiency; and 5) transformation efficiency via the lithium-acetate, bicine, and electroporation methods. On the basis of physiological as well as genetic properties, strains from the CEN.PK family were selected as a platform for cell-factory research on the stoichiometry and kinetics of growth and product formation.
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